FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3155776178> ?p ?o ?g. }

• W3155776178 endingPage "733" @default.
• W3155776178 startingPage "725" @default.
• W3155776178 abstract "The mammalian cell nucleus contains different types of membrane-less nuclear bodies (NBs) consisting of proteins and RNAs. Microscopic imaging has been widely applied to study the organization and structure of NBs. However, current fixation methods are not optimized for such imaging: When a fixation method is chosen to maximize the quality of the RNA fluorescence in situ hybridization (FISH), it often limits the labeling efficiency of proteins or affects the ultrastructure of NBs. Here, we report that addition of glyoxal (GO) into the classical paraformaldehyde (PFA) fixation step not only improves FISH signals for RNAs in NBs via augmented permeability of the fixed nucleus and enhanced accessibility of probes, but also largely preserves protein fluorescent signals during fixation and immunostaining. We also show that GO/PFA fixation enables the covisualization of different types of nuclear bodies with minimal impact on their ultrastructures under super-resolution microscopy." @default.
• W3155776178 created "2021-04-26" @default.
• W3155776178 creator A5064026516 @default.
• W3155776178 creator A5071369567 @default.
• W3155776178 creator A5079251794 @default.
• W3155776178 date "2021-04-12" @default.
• W3155776178 modified "2023-10-14" @default.
• W3155776178 title "An optimized fixation method containing glyoxal and paraformaldehyde for imaging nuclear bodies" @default.
• W3155776178 cites W16623706 @default.
• W3155776178 cites W1967291882 @default.
• W3155776178 cites W1973241154 @default.
• W3155776178 cites W1983340804 @default.
• W3155776178 cites W2037957091 @default.
• W3155776178 cites W2048213102 @default.
• W3155776178 cites W2054760402 @default.
• W3155776178 cites W2059200934 @default.
• W3155776178 cites W2075112713 @default.
• W3155776178 cites W2103937847 @default.
• W3155776178 cites W2108012214 @default.
• W3155776178 cites W2109961952 @default.
• W3155776178 cites W2113374624 @default.
• W3155776178 cites W2522982067 @default.
• W3155776178 cites W2540143287 @default.
• W3155776178 cites W2610181528 @default.
• W3155776178 cites W2621367368 @default.
• W3155776178 cites W2769039165 @default.
• W3155776178 cites W2770336503 @default.
• W3155776178 cites W2806524706 @default.
• W3155776178 cites W2891242171 @default.
• W3155776178 cites W2898560457 @default.
• W3155776178 cites W2943588752 @default.
• W3155776178 cites W2951430642 @default.
• W3155776178 cites W2973637866 @default.
• W3155776178 cites W3087868350 @default.
• W3155776178 cites W4246488417 @default.
• W3155776178 doi "https://doi.org/10.1261/rna.078671.120" @default.
• W3155776178 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8127994" @default.
• W3155776178 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33846273" @default.
• W3155776178 hasPublicationYear "2021" @default.
• W3155776178 type Work @default.
• W3155776178 sameAs 3155776178 @default.
• W3155776178 citedByCount "4" @default.
• W3155776178 countsByYear W31557761782021 @default.
• W3155776178 countsByYear W31557761782023 @default.
• W3155776178 crossrefType "journal-article" @default.
• W3155776178 hasAuthorship W3155776178A5064026516 @default.
• W3155776178 hasAuthorship W3155776178A5071369567 @default.
• W3155776178 hasAuthorship W3155776178A5079251794 @default.
• W3155776178 hasBestOaLocation W31557761781 @default.
• W3155776178 hasConcept C104317684 @default.
• W3155776178 hasConcept C121332964 @default.
• W3155776178 hasConcept C12554922 @default.
• W3155776178 hasConcept C142724271 @default.
• W3155776178 hasConcept C146249460 @default.
• W3155776178 hasConcept C150194340 @default.
• W3155776178 hasConcept C178790620 @default.
• W3155776178 hasConcept C185592680 @default.
• W3155776178 hasConcept C190062978 @default.
• W3155776178 hasConcept C203014093 @default.
• W3155776178 hasConcept C204232928 @default.
• W3155776178 hasConcept C2777603720 @default.
• W3155776178 hasConcept C2777822432 @default.
• W3155776178 hasConcept C2778723968 @default.
• W3155776178 hasConcept C2780723820 @default.
• W3155776178 hasConcept C2780848204 @default.
• W3155776178 hasConcept C4224716 @default.
• W3155776178 hasConcept C55493867 @default.
• W3155776178 hasConcept C62520636 @default.
• W3155776178 hasConcept C67705224 @default.
• W3155776178 hasConcept C71924100 @default.
• W3155776178 hasConcept C81439078 @default.
• W3155776178 hasConcept C86803240 @default.
• W3155776178 hasConcept C91881484 @default.
• W3155776178 hasConcept C95444343 @default.
• W3155776178 hasConceptScore W3155776178C104317684 @default.
• W3155776178 hasConceptScore W3155776178C121332964 @default.
• W3155776178 hasConceptScore W3155776178C12554922 @default.
• W3155776178 hasConceptScore W3155776178C142724271 @default.
• W3155776178 hasConceptScore W3155776178C146249460 @default.
• W3155776178 hasConceptScore W3155776178C150194340 @default.
• W3155776178 hasConceptScore W3155776178C178790620 @default.
• W3155776178 hasConceptScore W3155776178C185592680 @default.
• W3155776178 hasConceptScore W3155776178C190062978 @default.
• W3155776178 hasConceptScore W3155776178C203014093 @default.
• W3155776178 hasConceptScore W3155776178C204232928 @default.
• W3155776178 hasConceptScore W3155776178C2777603720 @default.
• W3155776178 hasConceptScore W3155776178C2777822432 @default.
• W3155776178 hasConceptScore W3155776178C2778723968 @default.
• W3155776178 hasConceptScore W3155776178C2780723820 @default.
• W3155776178 hasConceptScore W3155776178C2780848204 @default.
• W3155776178 hasConceptScore W3155776178C4224716 @default.
• W3155776178 hasConceptScore W3155776178C55493867 @default.
• W3155776178 hasConceptScore W3155776178C62520636 @default.
• W3155776178 hasConceptScore W3155776178C67705224 @default.
• W3155776178 hasConceptScore W3155776178C71924100 @default.
• W3155776178 hasConceptScore W3155776178C81439078 @default.
• W3155776178 hasConceptScore W3155776178C86803240 @default.
• W3155776178 hasConceptScore W3155776178C91881484 @default.

• next