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• W3156080874 endingPage "104100" @default.
• W3156080874 startingPage "104100" @default.
• W3156080874 abstract "Toll-like receptors (TLRs), as a family of pattern recognition receptors (PRRs), possess specific pathogen-related molecular pattern (PAMP) recognition spectrum in inducing immune responses. In this study, sixteen TLRs were identified and characterized in mandarin fish (Siniperca chuatsi). All these TLRs consist of leucine-rich repeats (LRRs), a transmembrane domain and a Toll/interleukin-I receptor (TIR) domain, with the exception of TLR5S which lacks TIR domain, and they can be clustered into five branches, i.e. TLR1 subfamily, TLR3 subfamily, TLR5 subfamily, TLR7 subfamily and TLR11 subfamily in phylogenetic tree. These TLR genes were expressed in all tested tissues and had high expression levels in immune-related tissues such as head-kidney and spleen or mucosa-related tissues such as intestine and pyloric caecum. The transcripts of TLR2a, TLR2b, TLR3, TLR13a, TLR14, TLR22 and TLR23 were all significantly up-regulated after stimulation with poly(I:C); TLR1, TLR2a, TLR2b, TLR3, TLR5M, TLR5S, TLR13a and TLR13b transcripts were all significantly up-regulated after stimulation with PGN; and TLR2a, TLR2b, TLR5M, TLR5S, TLR7, TLR8, TLR9, TLR13c, TLR14 and TLR22 transcripts were all significantly up-regulated after stimulation with LPS in isolated head kidney lymphocytes of mandarin fish. The findings in this study may provide a valuable basis for functional study on TLR genes in mandarin fish." @default.
• W3156080874 created "2021-04-26" @default.
• W3156080874 creator A5044023031 @default.
• W3156080874 creator A5051875515 @default.
• W3156080874 creator A5065772933 @default.
• W3156080874 creator A5068708693 @default.
• W3156080874 date "2021-08-01" @default.
• W3156080874 modified "2023-10-14" @default.
• W3156080874 title "Identification and expression analysis of sixteen Toll-like receptor genes, TLR1, TLR2a, TLR2b, TLR3, TLR5M, TLR5S, TLR7−9, TLR13a−c, TLR14, TLR21−23 in mandarin fish Siniperca chuatsi" @default.
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• W3156080874 doi "https://doi.org/10.1016/j.dci.2021.104100" @default.
• W3156080874 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33862097" @default.

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