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• W3156089994 abstract "Abstract Motivation Fusarium langsethiae is a T-2 and HT-2 mycotoxins producing Fusarium species firstly characterised in 2004. It is commonly isolated from oats in Northern Europe. T-2 and HT-2 mycotoxins exhibit immunological and haemotological effects in animal health mainly through inhibition of protein, RNA and DNA synthesis. The development of a high-quality and comprehensively annotated assembly for this species is therefore essential in providing the molecular understanding and the mechanism of T-2 and HT-2 biosynthesis in F. langsethiae to help develop effective control strategies. Results The F. langsethiae assembly was produced using PacBio long reads, which were then assembled independently using Canu, SMARTdenovo and Flye; producing a genome assembly total length of 59Mb and N50 of 3.51Mb. A total of 19,336 coding genes were identified using RNA-Seq informed ab-initio gene prediction. Finally, predicting genes were annotated using the basic local alignment search tool (BLAST) against the NCBI non-redundant (NR) genome database and protein hits were annotated using InterProScan. Genes with blast hits were functionally annotated with Gene Ontology. Contact f.mohareb@cranfield.ac.uk Data availability Raw sequence reads and assembled genome can be downloaded from: GenBank under the accession JAFFKB000000000" @default.
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• W3156089994 date "2021-04-14" @default.
• W3156089994 modified "2023-10-17" @default.
• W3156089994 title "De novo genome assembly and functional annotation forFusarium langsethiae" @default.
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• W3156089994 doi "https://doi.org/10.1101/2021.04.13.439621" @default.
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