Matches in SemOpenAlex for { <https://semopenalex.org/work/W3156134196> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W3156134196 abstract "Metabolic engineering frequently requires both gene knockouts and gene integration. CRISPR-Cas9 has been extensively used to create double-stranded DNA breaks that result in indel mutations; however, such mutations can revert or create toxic product. Gene integration can also be accomplished by CRISPR-Cas9 introduced double-stranded DNA breaks and a donor DNA cassette. Here we describe our protocol for combining an efficient gene knockout created by introducing DNA cuts with two guide RNAs with a gene to be integrated at the knockout site. Including guide RNA target sites flanking the homology regions around the gene to be integrated enables both homology-directed repair and homology-mediated end joining, resulting in few deletions and a significant proportion of correctly knocked out and integrated genes." @default.
- W3156134196 created "2021-04-26" @default.
- W3156134196 creator A5006285317 @default.
- W3156134196 creator A5084618207 @default.
- W3156134196 date "2021-01-01" @default.
- W3156134196 modified "2023-09-23" @default.
- W3156134196 title "Simultaneous Gene Excision and Integration by Dual-Guide CRISPR-Cas9" @default.
- W3156134196 cites W1967893478 @default.
- W3156134196 cites W2012746361 @default.
- W3156134196 cites W2020481873 @default.
- W3156134196 cites W2115420193 @default.
- W3156134196 cites W2217013086 @default.
- W3156134196 cites W2560066302 @default.
- W3156134196 cites W2563320377 @default.
- W3156134196 cites W2572443119 @default.
- W3156134196 cites W2616802735 @default.
- W3156134196 cites W2784084148 @default.
- W3156134196 cites W2791084848 @default.
- W3156134196 cites W2804203222 @default.
- W3156134196 cites W2807097276 @default.
- W3156134196 cites W2807190680 @default.
- W3156134196 cites W2920862685 @default.
- W3156134196 cites W2952993212 @default.
- W3156134196 doi "https://doi.org/10.1007/978-1-0716-1414-3_4" @default.
- W3156134196 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33847982" @default.
- W3156134196 hasPublicationYear "2021" @default.
- W3156134196 type Work @default.
- W3156134196 sameAs 3156134196 @default.
- W3156134196 citedByCount "0" @default.
- W3156134196 crossrefType "book-chapter" @default.
- W3156134196 hasAuthorship W3156134196A5006285317 @default.
- W3156134196 hasAuthorship W3156134196A5084618207 @default.
- W3156134196 hasConcept C104317684 @default.
- W3156134196 hasConcept C104451858 @default.
- W3156134196 hasConcept C111829913 @default.
- W3156134196 hasConcept C119054055 @default.
- W3156134196 hasConcept C132455925 @default.
- W3156134196 hasConcept C134935766 @default.
- W3156134196 hasConcept C135763542 @default.
- W3156134196 hasConcept C144501496 @default.
- W3156134196 hasConcept C145290725 @default.
- W3156134196 hasConcept C153209595 @default.
- W3156134196 hasConcept C165525559 @default.
- W3156134196 hasConcept C54355233 @default.
- W3156134196 hasConcept C552990157 @default.
- W3156134196 hasConcept C70721500 @default.
- W3156134196 hasConcept C74054111 @default.
- W3156134196 hasConcept C77957584 @default.
- W3156134196 hasConcept C86803240 @default.
- W3156134196 hasConcept C97702854 @default.
- W3156134196 hasConcept C98108389 @default.
- W3156134196 hasConceptScore W3156134196C104317684 @default.
- W3156134196 hasConceptScore W3156134196C104451858 @default.
- W3156134196 hasConceptScore W3156134196C111829913 @default.
- W3156134196 hasConceptScore W3156134196C119054055 @default.
- W3156134196 hasConceptScore W3156134196C132455925 @default.
- W3156134196 hasConceptScore W3156134196C134935766 @default.
- W3156134196 hasConceptScore W3156134196C135763542 @default.
- W3156134196 hasConceptScore W3156134196C144501496 @default.
- W3156134196 hasConceptScore W3156134196C145290725 @default.
- W3156134196 hasConceptScore W3156134196C153209595 @default.
- W3156134196 hasConceptScore W3156134196C165525559 @default.
- W3156134196 hasConceptScore W3156134196C54355233 @default.
- W3156134196 hasConceptScore W3156134196C552990157 @default.
- W3156134196 hasConceptScore W3156134196C70721500 @default.
- W3156134196 hasConceptScore W3156134196C74054111 @default.
- W3156134196 hasConceptScore W3156134196C77957584 @default.
- W3156134196 hasConceptScore W3156134196C86803240 @default.
- W3156134196 hasConceptScore W3156134196C97702854 @default.
- W3156134196 hasConceptScore W3156134196C98108389 @default.
- W3156134196 hasLocation W31561341961 @default.
- W3156134196 hasOpenAccess W3156134196 @default.
- W3156134196 hasPrimaryLocation W31561341961 @default.
- W3156134196 hasRelatedWork W10791717 @default.
- W3156134196 hasRelatedWork W14581506 @default.
- W3156134196 hasRelatedWork W19048415 @default.
- W3156134196 hasRelatedWork W19386517 @default.
- W3156134196 hasRelatedWork W28934323 @default.
- W3156134196 hasRelatedWork W30659687 @default.
- W3156134196 hasRelatedWork W3116027 @default.
- W3156134196 hasRelatedWork W38248087 @default.
- W3156134196 hasRelatedWork W39309114 @default.
- W3156134196 hasRelatedWork W800577 @default.
- W3156134196 isParatext "false" @default.
- W3156134196 isRetracted "false" @default.
- W3156134196 magId "3156134196" @default.
- W3156134196 workType "book-chapter" @default.