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• W3156278519 abstract "Purpose: Lipids play vital roles in cellular function and signaling. The lipidome dynamically adapts to physiological and pathological conditions. In turn, lipidomics analysis has become a valuable technology for understanding patho-physiological mechanisms and the identification of candidate biomarkers. Variability in within-subject repeated measurements may lead to bias towards the null when estimating the association between biomarkers and a disease or treatment. To estimate the variability of the measurement, information regarding the stability of the metabolite levels over time is essential. Therefore, we aimed to assess the lipid composition and biological reproducibility of lipid measurements in plasma and erythrocytes. Methods: Blood samples were obtained from 42 placebo-treated hand osteoarthritis patients (77% women, mean age 65 years, mean BMI 27 kg/m2), included in the Hand Osteoarthritis Prednisolone Efficacy (HOPE) study. Samples were obtained non-fasted at baseline and six weeks. From each blood sample, plasma and erythrocytes were separated and used for the quantitative measurement of up to 1000 lipid species across 13 lipid classes with the LipidyzerTM platform in nmol/mL. Data was processed based on the relative standard deviation of quality controls, taking batch effects into account. Intraclass correlation coefficients (ICCs) and corresponding 95% confidence intervals (CI) were calculated to investigate the variability of the lipid concentrations between timepoints. The ICC distribution of lipid metabolites in plasma and erythrocytes were compared using two-sided paired Wilcoxon tests. Results: We measured 778 lipids in plasma, compared to 916 lipids in erythrocytes. After data processing, the analyses included 630 lipids in plasma, and 286 in erythrocytes. From these, 243 lipids overlapped between sample types. Major differences were observed between the sample types in the number of lipids per lipid class and the total concentration of the lipids within a class. Triacylglycerols (TAG) and cholesteryl esters (CE) were much more abundant in plasma. Conversely, phosphatidylethanolamines (PE), sphingomyelins (SM) and ceramides (CER) were less abundant in plasma compared to erythrocytes (Table 1). In plasma 78% of lipid measurements were good to excellently reproduced, with an overall median ICC of 0.69. Compared to plasma, a considerably lower amount (35%) of lipids were well reproducible in erythrocytes. Median reproducibility of lipids in erythrocytes was 0.51 (0.24-0.79). Figure 1 shows a comparison of the ICC score distribution in plasma with erythrocytes, with a significantly better reproducibility in plasma (p-value<0.001). However, while overall reproducibility was better in plasma, this was not observed for all lipid classes. At class-level, reproducibility in plasma was superior for TAGs and CEs, while CERs, DAGs, (L)PEs and SMs showed better reproducibility in erythrocytes.Table 1Number of individual lipids per class and class concentrations in plasma and erythrocytesPlasmaErythrocytesNumber of lipid speciesClass concentration (nmol/mL)Number of lipid speciesClass concentration (nmol/mL)Triacylglycerols4821579.4 (1064.9-3195.2)1346.5 (5.6-9.4)Diacylglycerols913.3 (8.4-22.2)105.8 (4.7-6.2)Free fatty acids20745.3 (552.0-1202.9)20486.9 (379.2-669.2)Cholesteryl esters244571.6 (4065.1-5521.3)51.2 (0.9-1.7)Phosphatidylcholines314013.7 (3203.1-4661.6)423899.2 (3723.0-4296.6)Phosphatidylethanolamines26156.2 (120.9-180.3)423954.6 (3721.9-4323.3)Lysophosphatidylcholines9385.9 (335.6-442.9)7119.8 (109.7-168.9)Lysophosphatidylethanolamines24.2 (3.5-4.9)48.6 (6.8-9.7)Sphingomyelins121204.6 (1037.0-1351.9)82695.8 (2434.8-2815.6)Ceramides614.1 (11.9-17.4)7163.0 (133.3-186.4)Dihydroceramides21.0 (0.8-1.3)11.8 (1.4-2.1)Hexosylceramides55.1 (4.7-5.9)45.6 (5.0-7.4)Lactosylceramides23.4 (2.7-3.8)223.8 (20.6-33.5)Numbers represent median (interquartile range) unless otherwise specified. Data represents baseline measurements. Open table in a new tab Numbers represent median (interquartile range) unless otherwise specified. Data represents baseline measurements. Conclusions: In plasma biological reproducibility was good for most lipid measurements. Although overall reproducibility was better in plasma compared to erythrocytes, notable differences were observed at individual- and lipid class-level that may favour the use of a particular sample type." @default.
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• W3156278519 date "2021-04-01" @default.
• W3156278519 modified "2023-10-16" @default.
• W3156278519 title "Biological reproducibility of targeted lipidome analyses (LipidyzerTM) in plasma and erythrocytes over a 6-week period" @default.
• W3156278519 doi "https://doi.org/10.1016/j.joca.2021.02.201" @default.
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