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• W3156436437 startingPage "844" @default.
• W3156436437 abstract "Genome editing in bacteria encompasses a wide array of laborious and multi-step methods such as suicide plasmids. The discovery and applications of clustered regularly interspaced short palindromic repeats (CRISPR)-Cas based technologies have revolutionized genome editing in eukaryotic organisms due to its simplicity and programmability. Nevertheless, this system has not been as widely favored for bacterial genome editing. In this review, we summarize the main approaches and difficulties associated with CRISPR-Cas-mediated genome editing in bacteria and present some alternatives to circumvent these issues, including CRISPR nickases, Cas12a, base editors, CRISPR-associated transposases, prime-editing, endogenous CRISPR systems, and the use of pre-made ribonucleoprotein complexes of Cas proteins and guide RNAs. Finally, we also address fluorescent-protein-based methods to evaluate the efficacy of CRISPR-based systems for genome editing in bacteria. CRISPR-Cas still holds promise as a generalized genome-editing tool in bacteria and is developing further optimization for an expanded application in these organisms. This review provides a rarely offered comprehensive view of genome editing. It also aims to familiarize the microbiology community with an ever-growing genome-editing toolbox for bacteria." @default.
• W3156436437 created "2021-04-26" @default.
• W3156436437 creator A5022044970 @default.
• W3156436437 creator A5038400022 @default.
• W3156436437 creator A5055249312 @default.
• W3156436437 date "2021-04-15" @default.
• W3156436437 modified "2023-10-16" @default.
• W3156436437 title "Genome Editing in Bacteria: CRISPR-Cas and Beyond" @default.
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