FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3156659732> ?p ?o ?g. }

• W3156659732 abstract "Abstract Encapsulins (Enc) are protein nanocompartments which house various cargo enzymes, including a family of decameric ferritin-like proteins. Previously, we elucidated the structure and activity of these ferritin-like proteins (EncFtn) and demonstrated that they must be encapsulated in a nanocompartment for iron storage. Here, we study a recombinant Haliangium ochraceum Enc:EncFtn complex using electron cryo-microscopy (Cryo-EM) and hydrogen/deuterium exchange mass spectrometry (HDX-MS) to gain insight into the structural relationship between Enc and EncFtn. An asymmetric single particle reconstruction reveals four EncFtn decamers in a tetrahedral arrangement within the encapsulin nanocompartment. This leads to a symmetry mismatch between the EncFtn cargo and the icosahedral encapsulin shell. The EncFtn decamers are offset from the interior face of the encapsulin shell and are resolved at a much lower overall resolution in the final reconstruction. This flexibility, and the fixed number of EncFtn decamers sequestered within, implies that the loading of the encapsulin nanocompartment is limited by the steric effect of both engaged and free encapsulin localization sequences. Using a combination of focused refinements and HDX-MS, we observed dynamic behavior of the major five-fold pore, and show the pore opening via the movement of the encapsulin A-domain. These data can accelerate efforts to engineer the sequestration of heterologous cargo proteins and to alter the permeability of the encapsulin shell via pore modifications." @default.
• W3156659732 created "2021-04-26" @default.
• W3156659732 creator A5010362694 @default.
• W3156659732 creator A5014447386 @default.
• W3156659732 creator A5019274470 @default.
• W3156659732 creator A5021020124 @default.
• W3156659732 creator A5027216964 @default.
• W3156659732 creator A5030526952 @default.
• W3156659732 creator A5037190623 @default.
• W3156659732 creator A5041492350 @default.
• W3156659732 creator A5052950019 @default.
• W3156659732 creator A5055262322 @default.
• W3156659732 creator A5060586573 @default.
• W3156659732 creator A5062324575 @default.
• W3156659732 creator A5066157232 @default.
• W3156659732 creator A5069823395 @default.
• W3156659732 creator A5082831672 @default.
• W3156659732 date "2021-04-15" @default.
• W3156659732 modified "2023-09-24" @default.
• W3156659732 title "Pore dynamics and asymmetric cargo loading in an encapsulin nanocompartment revealed by Cryo-EM and hydrogen/deuterium exchange mass spectrometry" @default.
• W3156659732 cites W1504760874 @default.
• W3156659732 cites W1769506315 @default.
• W3156659732 cites W1803102843 @default.
• W3156659732 cites W1838872057 @default.
• W3156659732 cites W1964537087 @default.
• W3156659732 cites W1970229578 @default.
• W3156659732 cites W1983390804 @default.
• W3156659732 cites W2000315054 @default.
• W3156659732 cites W2001715545 @default.
• W3156659732 cites W2011374678 @default.
• W3156659732 cites W2046855017 @default.
• W3156659732 cites W2052555699 @default.
• W3156659732 cites W2097545999 @default.
• W3156659732 cites W2106246537 @default.
• W3156659732 cites W2124026197 @default.
• W3156659732 cites W2142670773 @default.
• W3156659732 cites W2147537878 @default.
• W3156659732 cites W2154714625 @default.
• W3156659732 cites W2163341755 @default.
• W3156659732 cites W2167279371 @default.
• W3156659732 cites W2189955651 @default.
• W3156659732 cites W2466775628 @default.
• W3156659732 cites W2476390359 @default.
• W3156659732 cites W2587625522 @default.
• W3156659732 cites W2593304916 @default.
• W3156659732 cites W2593511418 @default.
• W3156659732 cites W2613173058 @default.
• W3156659732 cites W2734356283 @default.
• W3156659732 cites W2768468117 @default.
• W3156659732 cites W2786390877 @default.
• W3156659732 cites W2791670278 @default.
• W3156659732 cites W2810440784 @default.
• W3156659732 cites W2893225011 @default.
• W3156659732 cites W2899489314 @default.
• W3156659732 cites W2899714098 @default.
• W3156659732 cites W2950681580 @default.
• W3156659732 cites W2952923211 @default.
• W3156659732 cites W2952935831 @default.
• W3156659732 cites W2953320394 @default.
• W3156659732 cites W2955168413 @default.
• W3156659732 cites W2962608495 @default.
• W3156659732 cites W2973457155 @default.
• W3156659732 cites W3006486805 @default.
• W3156659732 cites W3026605719 @default.
• W3156659732 cites W3081726774 @default.
• W3156659732 cites W3083406432 @default.
• W3156659732 cites W3087271061 @default.
• W3156659732 cites W3093217454 @default.
• W3156659732 cites W3121755783 @default.
• W3156659732 cites W3131760520 @default.
• W3156659732 cites W3138711941 @default.
• W3156659732 cites W3140202536 @default.
• W3156659732 cites W3152553194 @default.
• W3156659732 cites W3158548760 @default.
• W3156659732 cites W3192984354 @default.
• W3156659732 hasPublicationYear "2021" @default.
• W3156659732 type Work @default.
• W3156659732 sameAs 3156659732 @default.
• W3156659732 citedByCount "0" @default.
• W3156659732 crossrefType "posted-content" @default.
• W3156659732 hasAuthorship W3156659732A5010362694 @default.
• W3156659732 hasAuthorship W3156659732A5014447386 @default.
• W3156659732 hasAuthorship W3156659732A5019274470 @default.
• W3156659732 hasAuthorship W3156659732A5021020124 @default.
• W3156659732 hasAuthorship W3156659732A5027216964 @default.
• W3156659732 hasAuthorship W3156659732A5030526952 @default.
• W3156659732 hasAuthorship W3156659732A5037190623 @default.
• W3156659732 hasAuthorship W3156659732A5041492350 @default.
• W3156659732 hasAuthorship W3156659732A5052950019 @default.
• W3156659732 hasAuthorship W3156659732A5055262322 @default.
• W3156659732 hasAuthorship W3156659732A5060586573 @default.
• W3156659732 hasAuthorship W3156659732A5062324575 @default.
• W3156659732 hasAuthorship W3156659732A5066157232 @default.
• W3156659732 hasAuthorship W3156659732A5069823395 @default.
• W3156659732 hasAuthorship W3156659732A5082831672 @default.
• W3156659732 hasBestOaLocation W31566597321 @default.
• W3156659732 hasConcept C121332964 @default.
• W3156659732 hasConcept C12554922 @default.
• W3156659732 hasConcept C162356407 @default.
• W3156659732 hasConcept C167569220 @default.

• next