FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3157154698> ?p ?o ?g. }

• W3157154698 endingPage "1076" @default.
• W3157154698 startingPage "1076" @default.
• W3157154698 abstract "Fluorescent reporter mouse lines and Cre/Flp recombinase driver lines play essential roles in investigating various molecular functions in vivo. Now that applications of the CRISPR/Cas9 genome-editing system to mouse fertilized eggs have drastically accelerated these knock-in mouse generations, the next need is to establish easier, quicker, and cheaper methods for knock-in donor preparation. Here, we reverify and optimize the phospho-PCR method to obtain highly pure long single-stranded DNAs (ssDNAs) suitable for knock-in mouse generation via genome editing. The sophisticated sequential use of two exonucleases, in which double-stranded DNAs (dsDNAs) amplified by a pair of 5'-phosphorylated primer and normal primer are digested by Lambda exonuclease to yield ssDNA and the following Exonuclease III treatment degrades the remaining dsDNAs, enables much easier long ssDNA productions without laborious gel extraction steps. By microinjecting these donor DNAs along with CRISPR/Cas9 components into mouse zygotes, we have effectively generated fluorescent reporter lines and recombinase drivers. To further broaden the applicability, we have prepared long ssDNA donors in higher concentrations and electroporated them into mouse eggs to successfully obtain knock-in embryos. This classical yet improved method, which is regaining attention on the progress of CRISPR/Cas9 development, shall be the first choice for long donor DNA preparation, and the resulting knock-in lines could accelerate life science research." @default.
• W3157154698 created "2021-05-10" @default.
• W3157154698 creator A5010243611 @default.
• W3157154698 creator A5010711974 @default.
• W3157154698 creator A5017610382 @default.
• W3157154698 creator A5018232521 @default.
• W3157154698 creator A5018280195 @default.
• W3157154698 creator A5019716453 @default.
• W3157154698 creator A5024693827 @default.
• W3157154698 creator A5032709542 @default.
• W3157154698 creator A5033530353 @default.
• W3157154698 creator A5043840252 @default.
• W3157154698 creator A5044080699 @default.
• W3157154698 creator A5064145779 @default.
• W3157154698 creator A5080922686 @default.
• W3157154698 date "2021-04-30" @default.
• W3157154698 modified "2023-10-16" @default.
• W3157154698 title "An Optimized Preparation Method for Long ssDNA Donors to Facilitate Quick Knock-In Mouse Generation" @default.
• W3157154698 cites W1091016337 @default.
• W3157154698 cites W1658221551 @default.
• W3157154698 cites W1963911559 @default.
• W3157154698 cites W2016012222 @default.
• W3157154698 cites W2018340379 @default.
• W3157154698 cites W2019749991 @default.
• W3157154698 cites W2033680024 @default.
• W3157154698 cites W2040143158 @default.
• W3157154698 cites W2063511140 @default.
• W3157154698 cites W2088745158 @default.
• W3157154698 cites W2103794559 @default.
• W3157154698 cites W2114783261 @default.
• W3157154698 cites W2140754284 @default.
• W3157154698 cites W2283705032 @default.
• W3157154698 cites W2484900834 @default.
• W3157154698 cites W2604576157 @default.
• W3157154698 cites W2614850398 @default.
• W3157154698 cites W2777434339 @default.
• W3157154698 cites W2803479625 @default.
• W3157154698 cites W2804655893 @default.
• W3157154698 cites W2810570685 @default.
• W3157154698 cites W2818540548 @default.
• W3157154698 cites W2897950045 @default.
• W3157154698 cites W2952097794 @default.
• W3157154698 cites W2989635394 @default.
• W3157154698 cites W3081507281 @default.
• W3157154698 cites W3092693572 @default.
• W3157154698 doi "https://doi.org/10.3390/cells10051076" @default.
• W3157154698 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8147208" @default.
• W3157154698 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33946570" @default.
• W3157154698 hasPublicationYear "2021" @default.
• W3157154698 type Work @default.
• W3157154698 sameAs 3157154698 @default.
• W3157154698 citedByCount "10" @default.
• W3157154698 countsByYear W31571546982021 @default.
• W3157154698 countsByYear W31571546982022 @default.
• W3157154698 countsByYear W31571546982023 @default.
• W3157154698 crossrefType "journal-article" @default.
• W3157154698 hasAuthorship W3157154698A5010243611 @default.
• W3157154698 hasAuthorship W3157154698A5010711974 @default.
• W3157154698 hasAuthorship W3157154698A5017610382 @default.
• W3157154698 hasAuthorship W3157154698A5018232521 @default.
• W3157154698 hasAuthorship W3157154698A5018280195 @default.
• W3157154698 hasAuthorship W3157154698A5019716453 @default.
• W3157154698 hasAuthorship W3157154698A5024693827 @default.
• W3157154698 hasAuthorship W3157154698A5032709542 @default.
• W3157154698 hasAuthorship W3157154698A5033530353 @default.
• W3157154698 hasAuthorship W3157154698A5043840252 @default.
• W3157154698 hasAuthorship W3157154698A5044080699 @default.
• W3157154698 hasAuthorship W3157154698A5064145779 @default.
• W3157154698 hasAuthorship W3157154698A5080922686 @default.
• W3157154698 hasBestOaLocation W31571546981 @default.
• W3157154698 hasConcept C102230213 @default.
• W3157154698 hasConcept C102414288 @default.
• W3157154698 hasConcept C104317684 @default.
• W3157154698 hasConcept C125929170 @default.
• W3157154698 hasConcept C132455925 @default.
• W3157154698 hasConcept C141035611 @default.
• W3157154698 hasConcept C141231307 @default.
• W3157154698 hasConcept C144501496 @default.
• W3157154698 hasConcept C153911025 @default.
• W3157154698 hasConcept C156695909 @default.
• W3157154698 hasConcept C178790620 @default.
• W3157154698 hasConcept C185592680 @default.
• W3157154698 hasConcept C2777501222 @default.
• W3157154698 hasConcept C2777563447 @default.
• W3157154698 hasConcept C2781243718 @default.
• W3157154698 hasConcept C2796436 @default.
• W3157154698 hasConcept C4918238 @default.
• W3157154698 hasConcept C54355233 @default.
• W3157154698 hasConcept C547475151 @default.
• W3157154698 hasConcept C552990157 @default.
• W3157154698 hasConcept C7029365 @default.
• W3157154698 hasConcept C70721500 @default.
• W3157154698 hasConcept C82381507 @default.
• W3157154698 hasConcept C86803240 @default.
• W3157154698 hasConcept C95444343 @default.
• W3157154698 hasConcept C98108389 @default.
• W3157154698 hasConceptScore W3157154698C102230213 @default.
• W3157154698 hasConceptScore W3157154698C102414288 @default.

• next