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- W3157156634 endingPage "338567" @default.
- W3157156634 startingPage "338567" @default.
- W3157156634 abstract "Acute myocardial infarction (AMI) is the leading cause of morbidity and mortality globally. The serum levels of a group of cardiac biomarkers have been regarded as important indicators in the routine diagnosis of AMI. The development of rapid, sensitive, and accurate detection methods of AMI biomarkers is urgently needed for the early diagnosis of AMI. Here, a dynamic and pseudo-homogeneous enzyme-linked immunosorbent assay (ELISA) was reported based on the combined use of bioconjugated magnetic nanochains (MNCs) and gold nanoparticles (AuNPs) probes. The capture antibodies-conjugated MNCs served as dynamic nano-mixers to facilitate liquid mixing and as homogeneously dispersed capturing agents to capture and separate specific targets. The AuNPs probes were prepared by co-immobilization of detection antibodies and horseradish peroxidase (HRP) for signals amplification. The design of bioconjugated MNCs and AuNPs probes significantly increased the assay kinetics and improves the assay sensitivity. This novel ELISA strategy realized accurate detection of a panel of AMI biomarkers within 35 min, leading to considerably improved sensitivities compared to that of conventional ELISA method." @default.
- W3157156634 created "2021-05-10" @default.
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- W3157156634 date "2021-06-01" @default.
- W3157156634 modified "2023-09-23" @default.
- W3157156634 title "Magnetic nanochains-based dynamic ELISA for rapid and ultrasensitive detection of acute myocardial infarction biomarkers" @default.
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- W3157156634 doi "https://doi.org/10.1016/j.aca.2021.338567" @default.
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