FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3157196511> ?p ?o ?g. }

• W3157196511 endingPage "e0245407" @default.
• W3157196511 startingPage "e0245407" @default.
• W3157196511 abstract "Within the field of bioproduction, non-model organisms offer promise as bio-platform candidates. Non-model organisms can possess natural abilities to consume complex feedstocks, produce industrially useful chemicals, and withstand extreme environments that can be ideal for product extraction. However, non-model organisms also come with unique challenges due to lack of characterization. As a consequence, developing synthetic biology tools, predicting growth behavior, and building computational models can be difficult. There have been many advancements that have improved work with non-model organisms to address broad limitations, however each organism can come with unique surprises. Here we share our work in the non-model bacterium Actinobacillus succinognes 130Z, which includes both advancements in synthetic biology toolkit development and pitfalls in unpredictable fermentation behaviors. To develop a synthetic biology “tool kit” for A . succinogenes , information gleaned from a growth study and antibiotic screening was used to characterize 22 promoters which demonstrated a 260-fold range of fluorescence protein expression. The strongest of the promoters was incorporated into an inducible system for tunable gene control in A . succinogenes using the promoter for the lac operon as a template. This system flaunted a 481-fold range of expression and no significant basal expression. These findings were accompanied by unexpected changes in fermentation products characterized by a loss of succinic acid and increase in lactic acid after approximately 10 months in the lab. During evaluation of the fermentation shifts, new tests of the synthetic biology tools in a succinic acid producing strain revealed a significant loss in their functionality. Contamination and mutation were ruled out as causes and further testing is needed to elucidate the driving factors. The significance of this work is to share a successful tool development strategy that could be employed in other non-model species, report on an unfortunate phenomenon that needs addressed for further development of A . succinogenes , and provide a cautionary tale for those undertaking non-model research. In sharing our findings, we seek to provide tools and necessary information for further development of A . succinogenes as a platform for bioproduction of succinic acid and to illustrate the importance of diligent and long-term observation when working with non-model bacteria." @default.
• W3157196511 created "2021-05-10" @default.
• W3157196511 creator A5000324196 @default.
• W3157196511 creator A5001131365 @default.
• W3157196511 creator A5036731214 @default.
• W3157196511 creator A5053005768 @default.
• W3157196511 creator A5089064325 @default.
• W3157196511 date "2021-05-03" @default.
• W3157196511 modified "2023-10-17" @default.
• W3157196511 title "One step forward, two steps back: Transcriptional advancements and fermentation phenomena in Actinobacillus succinogenes 130Z" @default.
• W3157196511 cites W1446530232 @default.
• W3157196511 cites W1499482268 @default.
• W3157196511 cites W1505688690 @default.
• W3157196511 cites W1508088443 @default.
• W3157196511 cites W1943504827 @default.
• W3157196511 cites W1957496223 @default.
• W3157196511 cites W1970740144 @default.
• W3157196511 cites W1984528951 @default.
• W3157196511 cites W1985364973 @default.
• W3157196511 cites W1985620503 @default.
• W3157196511 cites W1987091690 @default.
• W3157196511 cites W1987643155 @default.
• W3157196511 cites W1994594395 @default.
• W3157196511 cites W2001394772 @default.
• W3157196511 cites W2002605655 @default.
• W3157196511 cites W2012537159 @default.
• W3157196511 cites W2025084644 @default.
• W3157196511 cites W2028603053 @default.
• W3157196511 cites W2032579724 @default.
• W3157196511 cites W2036917159 @default.
• W3157196511 cites W2051965776 @default.
• W3157196511 cites W2073224639 @default.
• W3157196511 cites W2080233018 @default.
• W3157196511 cites W2087823077 @default.
• W3157196511 cites W2099185000 @default.
• W3157196511 cites W2107523606 @default.
• W3157196511 cites W2108234281 @default.
• W3157196511 cites W2109867994 @default.
• W3157196511 cites W2124102845 @default.
• W3157196511 cites W2124985265 @default.
• W3157196511 cites W2128149989 @default.
• W3157196511 cites W2132759650 @default.
• W3157196511 cites W2138820253 @default.
• W3157196511 cites W2147686272 @default.
• W3157196511 cites W2149202606 @default.
• W3157196511 cites W2171852113 @default.
• W3157196511 cites W2180716231 @default.
• W3157196511 cites W2324591890 @default.
• W3157196511 cites W2333477409 @default.
• W3157196511 cites W2416290591 @default.
• W3157196511 cites W2461514601 @default.
• W3157196511 cites W2625552363 @default.
• W3157196511 cites W2766189659 @default.
• W3157196511 cites W2781978265 @default.
• W3157196511 cites W2806269398 @default.
• W3157196511 cites W2919796106 @default.
• W3157196511 doi "https://doi.org/10.1371/journal.pone.0245407" @default.
• W3157196511 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8092802" @default.
• W3157196511 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33939701" @default.
• W3157196511 hasPublicationYear "2021" @default.
• W3157196511 type Work @default.
• W3157196511 sameAs 3157196511 @default.
• W3157196511 citedByCount "2" @default.
• W3157196511 countsByYear W31571965112022 @default.
• W3157196511 countsByYear W31571965112023 @default.
• W3157196511 crossrefType "journal-article" @default.
• W3157196511 hasAuthorship W3157196511A5000324196 @default.
• W3157196511 hasAuthorship W3157196511A5001131365 @default.
• W3157196511 hasAuthorship W3157196511A5036731214 @default.
• W3157196511 hasAuthorship W3157196511A5053005768 @default.
• W3157196511 hasAuthorship W3157196511A5089064325 @default.
• W3157196511 hasBestOaLocation W31571965111 @default.
• W3157196511 hasConcept C100544194 @default.
• W3157196511 hasConcept C104317684 @default.
• W3157196511 hasConcept C127413603 @default.
• W3157196511 hasConcept C150903083 @default.
• W3157196511 hasConcept C152662350 @default.
• W3157196511 hasConcept C183696295 @default.
• W3157196511 hasConcept C191908910 @default.
• W3157196511 hasConcept C19843653 @default.
• W3157196511 hasConcept C2779227428 @default.
• W3157196511 hasConcept C54355233 @default.
• W3157196511 hasConcept C55493867 @default.
• W3157196511 hasConcept C6350086 @default.
• W3157196511 hasConcept C70721500 @default.
• W3157196511 hasConcept C86803240 @default.
• W3157196511 hasConceptScore W3157196511C100544194 @default.
• W3157196511 hasConceptScore W3157196511C104317684 @default.
• W3157196511 hasConceptScore W3157196511C127413603 @default.
• W3157196511 hasConceptScore W3157196511C150903083 @default.
• W3157196511 hasConceptScore W3157196511C152662350 @default.
• W3157196511 hasConceptScore W3157196511C183696295 @default.
• W3157196511 hasConceptScore W3157196511C191908910 @default.
• W3157196511 hasConceptScore W3157196511C19843653 @default.
• W3157196511 hasConceptScore W3157196511C2779227428 @default.
• W3157196511 hasConceptScore W3157196511C54355233 @default.
• W3157196511 hasConceptScore W3157196511C55493867 @default.
• W3157196511 hasConceptScore W3157196511C6350086 @default.

• next