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- W3157341570 abstract "Abstract Background: Propionic acid (PA), a potential building block for C3-based bulk chemicals, is used as a food preservative and antifungal agent because of the antimicrobial properties of its calcium-, potassium-, and sodium salts, as well as in the manufacture of pharmaceuticals, perfumes, pesticides and fungicides. However, industrial development of PA is seriously inhibited by oxygen stress, acid stress and glucose-induced osmotic stress concentration on account of the characteristic of Propionibacterium acidipropionici . To alleviate inhibition and increase PA production, enhancement P. acidipropionici tolerance to cross stress may be an effective strategy. Results: In this study, we first performed a combination of metabolic engineering (deletion of ldh and poxB and overexpression of mmc ) with evolutionary engineering (selection under oxygen stress, acid stress and osmotic stress) in P. acidipropionici . The results indicated the mutants received superior physiological activity, especially the mutant III exhibited steady 1.5-3.5 folds higher growth property and further 37.1% PA titer and 37.8% PA productivity increase than the wildtype. Moreover, omics analysis revealed the determinants such as Dps , GroES , dnaK , ADI and GAD referred to the acid adaptation of microbes were positively mobilized. ABC-type glycine betaine referred to the adaptability to osmotic stress was detected to be 2-4 folds up-regulated. More than 2-fold down-regulation of NADH oxidase and almost 3-fold up-regulation of SOD and POD were observed in three mutants. Moreover, an approximately 2.5-fold upregulation of mmc was also found.Conclusion: The multi-omics analysis revealed the multidirectional variation tendency of P. acidipropionici under cross stress and provided in-depth insights into the mechanism of tolerance and high production of PA, which layed the foundation for construction of microbial cell factories." @default.
- W3157341570 created "2021-05-10" @default.
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- W3157341570 date "2019-12-16" @default.
- W3157341570 modified "2023-09-23" @default.
- W3157341570 title "Integrating metabolic and evolutionary engineering for enhanced propionic acid production under cross stress: a multi-omics perspective" @default.
- W3157341570 doi "https://doi.org/10.21203/rs.2.18982/v1" @default.
- W3157341570 hasPublicationYear "2019" @default.
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