Matches in SemOpenAlex for { <https://semopenalex.org/work/W3157454976> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W3157454976 endingPage "47" @default.
- W3157454976 startingPage "35" @default.
- W3157454976 abstract "The role of podocytes is well conserved across species from drosophila to teleosts, and mammals. Identifying the molecular markers that actively maintain the integrity of the podocyte will enable a greater understanding of the changes that lead to damage.We generated transgenic zebrafish, expressing fluorescent reporters driven by the podocin promoter, for the visualization and isolation of podocytes. We have conducted single cell RNA sequencing (scRNA-seq) on isolated podocytes from a zebrafish reporter line.We demonstrated that the LifeAct-TagRFP-T fluorescent reporter faithfully replicated podocin expression in vivo. We were also able to show spontaneous GCaMP6s fluorescence using light sheet (single plane illumination) microscopy. We identified many podocyte transcripts, encoding proteins related to calcium-binding and actin filament assembly, in common with those expressed in human and mouse mature podocytes.We describe the establishment of novel transgenic zebrafish and their use to identify and isolate podocyte cells for the preparation of a scRNA-seq library from normal podocytes. The scRNA-seq data identifies distinct populations of cells and potential gene switching between clusters. These data provide a foundation for future comparative studies and for exploiting the zebrafish as a model for kidney development, disease, injury and repair." @default.
- W3157454976 created "2021-05-10" @default.
- W3157454976 creator A5015684615 @default.
- W3157454976 creator A5028717479 @default.
- W3157454976 creator A5042257244 @default.
- W3157454976 creator A5043238908 @default.
- W3157454976 creator A5044660998 @default.
- W3157454976 creator A5062533447 @default.
- W3157454976 creator A5065367596 @default.
- W3157454976 creator A5082154271 @default.
- W3157454976 creator A5089863644 @default.
- W3157454976 creator A5091451403 @default.
- W3157454976 date "2021-05-05" @default.
- W3157454976 modified "2023-09-27" @default.
- W3157454976 title "scRNA Transcription Profile of Adult Zebrafish Podocytes Using a Novel Reporter Strain" @default.
- W3157454976 doi "https://doi.org/10.33594/000000366" @default.
- W3157454976 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33945241" @default.
- W3157454976 hasPublicationYear "2021" @default.
- W3157454976 type Work @default.
- W3157454976 sameAs 3157454976 @default.
- W3157454976 citedByCount "3" @default.
- W3157454976 countsByYear W31574549762022 @default.
- W3157454976 countsByYear W31574549762023 @default.
- W3157454976 crossrefType "journal-article" @default.
- W3157454976 hasAuthorship W3157454976A5015684615 @default.
- W3157454976 hasAuthorship W3157454976A5028717479 @default.
- W3157454976 hasAuthorship W3157454976A5042257244 @default.
- W3157454976 hasAuthorship W3157454976A5043238908 @default.
- W3157454976 hasAuthorship W3157454976A5044660998 @default.
- W3157454976 hasAuthorship W3157454976A5062533447 @default.
- W3157454976 hasAuthorship W3157454976A5065367596 @default.
- W3157454976 hasAuthorship W3157454976A5082154271 @default.
- W3157454976 hasAuthorship W3157454976A5089863644 @default.
- W3157454976 hasAuthorship W3157454976A5091451403 @default.
- W3157454976 hasBestOaLocation W31574549761 @default.
- W3157454976 hasConcept C102230213 @default.
- W3157454976 hasConcept C104317684 @default.
- W3157454976 hasConcept C142613039 @default.
- W3157454976 hasConcept C153911025 @default.
- W3157454976 hasConcept C2776855237 @default.
- W3157454976 hasConcept C2776878037 @default.
- W3157454976 hasConcept C2777146197 @default.
- W3157454976 hasConcept C2779561371 @default.
- W3157454976 hasConcept C2780091579 @default.
- W3157454976 hasConcept C54355233 @default.
- W3157454976 hasConcept C86803240 @default.
- W3157454976 hasConcept C95444343 @default.
- W3157454976 hasConceptScore W3157454976C102230213 @default.
- W3157454976 hasConceptScore W3157454976C104317684 @default.
- W3157454976 hasConceptScore W3157454976C142613039 @default.
- W3157454976 hasConceptScore W3157454976C153911025 @default.
- W3157454976 hasConceptScore W3157454976C2776855237 @default.
- W3157454976 hasConceptScore W3157454976C2776878037 @default.
- W3157454976 hasConceptScore W3157454976C2777146197 @default.
- W3157454976 hasConceptScore W3157454976C2779561371 @default.
- W3157454976 hasConceptScore W3157454976C2780091579 @default.
- W3157454976 hasConceptScore W3157454976C54355233 @default.
- W3157454976 hasConceptScore W3157454976C86803240 @default.
- W3157454976 hasConceptScore W3157454976C95444343 @default.
- W3157454976 hasIssue "S4" @default.
- W3157454976 hasLocation W31574549761 @default.
- W3157454976 hasLocation W31574549762 @default.
- W3157454976 hasLocation W31574549763 @default.
- W3157454976 hasLocation W31574549764 @default.
- W3157454976 hasLocation W31574549765 @default.
- W3157454976 hasLocation W31574549766 @default.
- W3157454976 hasOpenAccess W3157454976 @default.
- W3157454976 hasPrimaryLocation W31574549761 @default.
- W3157454976 hasRelatedWork W2007199248 @default.
- W3157454976 hasRelatedWork W2034622268 @default.
- W3157454976 hasRelatedWork W2105769855 @default.
- W3157454976 hasRelatedWork W2109686700 @default.
- W3157454976 hasRelatedWork W2118827680 @default.
- W3157454976 hasRelatedWork W2376586511 @default.
- W3157454976 hasRelatedWork W2971175628 @default.
- W3157454976 hasRelatedWork W3157454976 @default.
- W3157454976 hasRelatedWork W3168902041 @default.
- W3157454976 hasRelatedWork W4375955663 @default.
- W3157454976 hasVolume "55" @default.
- W3157454976 isParatext "false" @default.
- W3157454976 isRetracted "false" @default.
- W3157454976 magId "3157454976" @default.
- W3157454976 workType "article" @default.