FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3157496664> ?p ?o ?g. }

• W3157496664 endingPage "28" @default.
• W3157496664 startingPage "28" @default.
• W3157496664 abstract "Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq) is a recently established multimodal single cell analysis technique combining the immunophenotyping capabilities of antibody labeling and cell sorting with the resolution of single-cell RNA sequencing (scRNA-seq). By simply adding a 12-bp nucleotide barcode to antibodies (cell hashing), CITE-seq can be used to sequence antibody-bound tags alongside the cellular mRNA, thus reducing costs of scRNA-seq by performing it at the same time on multiple barcoded samples in a single run. Here, we illustrate an ideal CITE-seq data analysis workflow by characterizing the transcriptome of SH-SY5Y neuroblastoma cell line, a widely used model to study neuronal function and differentiation. We obtained transcriptomes from a total of 2879 single cells, measuring an average of 1600 genes/cell. Along with standard scRNA-seq data handling procedures, such as quality checks and cell filtering procedures, we performed exploratory analyses to identify most stable genes to be possibly used as reference housekeeping genes in qPCR experiments. We also illustrate how to use some popular R packages to investigate cell heterogeneity in scRNA-seq data, namely Seurat, Monocle, and slalom. Both the CITE-seq dataset and the code used to analyze it are freely shared and fully reusable for future research." @default.
• W3157496664 created "2021-05-10" @default.
• W3157496664 creator A5003165331 @default.
• W3157496664 creator A5011478739 @default.
• W3157496664 creator A5034113263 @default.
• W3157496664 creator A5042294694 @default.
• W3157496664 creator A5060241787 @default.
• W3157496664 creator A5091800978 @default.
• W3157496664 date "2021-05-06" @default.
• W3157496664 modified "2023-10-04" @default.
• W3157496664 title "The Transcriptome of SH-SY5Y at Single-Cell Resolution: A CITE-Seq Data Analysis Workflow" @default.
• W3157496664 cites W1536601791 @default.
• W3157496664 cites W1965032353 @default.
• W3157496664 cites W1972816014 @default.
• W3157496664 cites W1984883254 @default.
• W3157496664 cites W2023799373 @default.
• W3157496664 cites W2030527512 @default.
• W3157496664 cites W2034752553 @default.
• W3157496664 cites W2100027949 @default.
• W3157496664 cites W2102102358 @default.
• W3157496664 cites W2105280376 @default.
• W3157496664 cites W2120876378 @default.
• W3157496664 cites W2149573463 @default.
• W3157496664 cites W2159115343 @default.
• W3157496664 cites W2159675211 @default.
• W3157496664 cites W2169456326 @default.
• W3157496664 cites W2184331293 @default.
• W3157496664 cites W2332292689 @default.
• W3157496664 cites W2601579284 @default.
• W3157496664 cites W2735067671 @default.
• W3157496664 cites W2739492614 @default.
• W3157496664 cites W2754794014 @default.
• W3157496664 cites W2767792097 @default.
• W3157496664 cites W2775437905 @default.
• W3157496664 cites W2785085486 @default.
• W3157496664 cites W2794480084 @default.
• W3157496664 cites W2800410670 @default.
• W3157496664 cites W2817838603 @default.
• W3157496664 cites W2886347923 @default.
• W3157496664 cites W2891826157 @default.
• W3157496664 cites W2946721812 @default.
• W3157496664 cites W2948517648 @default.
• W3157496664 cites W2953788278 @default.
• W3157496664 cites W2982380908 @default.
• W3157496664 cites W2988464219 @default.
• W3157496664 cites W3011804602 @default.
• W3157496664 cites W3013151148 @default.
• W3157496664 cites W3013348979 @default.
• W3157496664 cites W3027514154 @default.
• W3157496664 cites W3029931347 @default.
• W3157496664 cites W3037819346 @default.
• W3157496664 cites W3076758593 @default.
• W3157496664 cites W3102147110 @default.
• W3157496664 cites W3106188259 @default.
• W3157496664 cites W3122270106 @default.
• W3157496664 cites W3125524953 @default.
• W3157496664 cites W3131920572 @default.
• W3157496664 cites W3154635757 @default.
• W3157496664 doi "https://doi.org/10.3390/mps4020028" @default.
• W3157496664 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8163004" @default.
• W3157496664 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34066513" @default.
• W3157496664 hasPublicationYear "2021" @default.
• W3157496664 type Work @default.
• W3157496664 sameAs 3157496664 @default.
• W3157496664 citedByCount "2" @default.
• W3157496664 countsByYear W31574966642022 @default.
• W3157496664 crossrefType "journal-article" @default.
• W3157496664 hasAuthorship W3157496664A5003165331 @default.
• W3157496664 hasAuthorship W3157496664A5011478739 @default.
• W3157496664 hasAuthorship W3157496664A5034113263 @default.
• W3157496664 hasAuthorship W3157496664A5042294694 @default.
• W3157496664 hasAuthorship W3157496664A5060241787 @default.
• W3157496664 hasAuthorship W3157496664A5091800978 @default.
• W3157496664 hasBestOaLocation W31574966641 @default.
• W3157496664 hasConcept C104317684 @default.
• W3157496664 hasConcept C107397762 @default.
• W3157496664 hasConcept C150194340 @default.
• W3157496664 hasConcept C162317418 @default.
• W3157496664 hasConcept C177212765 @default.
• W3157496664 hasConcept C41008148 @default.
• W3157496664 hasConcept C54355233 @default.
• W3157496664 hasConcept C70721500 @default.
• W3157496664 hasConcept C77088390 @default.
• W3157496664 hasConcept C86803240 @default.
• W3157496664 hasConceptScore W3157496664C104317684 @default.
• W3157496664 hasConceptScore W3157496664C107397762 @default.
• W3157496664 hasConceptScore W3157496664C150194340 @default.
• W3157496664 hasConceptScore W3157496664C162317418 @default.
• W3157496664 hasConceptScore W3157496664C177212765 @default.
• W3157496664 hasConceptScore W3157496664C41008148 @default.
• W3157496664 hasConceptScore W3157496664C54355233 @default.
• W3157496664 hasConceptScore W3157496664C70721500 @default.
• W3157496664 hasConceptScore W3157496664C77088390 @default.
• W3157496664 hasConceptScore W3157496664C86803240 @default.
• W3157496664 hasIssue "2" @default.
• W3157496664 hasLocation W31574966641 @default.
• W3157496664 hasLocation W31574966642 @default.
• W3157496664 hasOpenAccess W3157496664 @default.

• next