FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3157553013> ?p ?o ?g. }

• W3157553013 endingPage "8" @default.
• W3157553013 startingPage "1" @default.
• W3157553013 abstract "Circulating Endothelial Cells (CECs) and Endothelial Progenitor Cells (EPCs) are gaining importance as quantifiable surrogate biomarkers of Endothelial Dysfunction (ED). Lack of a common definition and consequently, a standardized quantification method has limited clinical applicability of these biomarkers. A reliable, reproducible, and practicable method is required to achieve the full potential of these parameters. The objective of this study was to evaluate the test-retest reliability over a short period of time (~7 days) of flow cytometric quantification of CECs and EPCs in human whole blood from patients with cardiovascular disease. 100 patients (mean age 65 years ± 10 years, 30 women) were enrolled into a prospective study consisting of 4 patients groups: Heart Failure with reduced Ejection Fraction (HFrEF; n=25), Heart Failure with preserved Ejection Fraction (HFpEF; n=26), Diabetic Nephropathy (DN; n=25), and Hypertension (HTN; n=24). In addition, 11 healthy volunteers were included as a control group. At 2 study visits, a blood sample was drawn, which underwent an identical sequence of preparation and analysis. CECs (DNA+, CD45 dim, CD31+, and CD146+) and EPCs (CD45 dim, CD34 br, CD133+, and CD31+, FSC short-term test retest reliability, correlation (intraclass correlation) and agreement (Bland-Altman plot) of the measurements obtained at the 2 study visits were evaluated. Across all patients, median CECs/ml and EPCs/ml were 12 (5th/95th percentile: 6/22) and 679 (447/1281) at visit 1 and were 11 (6/24) and 736 (510/1105) at visit 2, respectively; Intraclass Correlation (ICC) was poor for CEC count (0.106; ICC-95% CI-0.08–0.29) and good for EPC count (0.9; 0.86– 0.93). In patients with HFpEF, ICC was poor for CEC count (0.294; 95% CI-0.08-0.6) and moderate in strength for EPC count (0.694; 0.43–0.85). In patients with HFrEF, ICC was poor for CEC count (0.076; -0.32–0.45) and excellent in strength for EPC count (0.946; 0.88–0.98). In patients with DN, ICC was poor for CEC count (-0.031; -0.44–0.37) and excellent in strength for EPC count (0.946; 0.88–0.98). In patients with HTN, ICC was poor for CEC count (0.143; -0.27–0.51) and moderate in strength for EPC count (0.668; 0.37–0.84). In healthy controls, ICC was poor for CEC count (0.378-0.26–0.78) and good in strength for EPC count (0.846; 0.59–0.96). A Bland-Altman plot showed a positive correlation of variations of differences and increasing median CEC counts; there were no distinct trends for median EPC counts. Our analyses indicate that flow cytometric quantification of EPC concentrations is reliable in patients with HFpEF, HFrEF, DN, and HTN. Quantification of CEC concentrations showed poor test-retest reliability across all patient groups. Further research is necessary to elucidate the nature of this finding, which could be due to higher biological variability in patients with severe ED. Clinical Trial Registration Identifier: NCT02299960" @default.
• W3157553013 created "2021-05-10" @default.
• W3157553013 creator A5017858830 @default.
• W3157553013 creator A5042631889 @default.
• W3157553013 creator A5045374209 @default.
• W3157553013 creator A5049347390 @default.
• W3157553013 creator A5069025004 @default.
• W3157553013 creator A5073076418 @default.
• W3157553013 creator A5075618911 @default.
• W3157553013 creator A5079924425 @default.
• W3157553013 creator A5082519884 @default.
• W3157553013 creator A5086287347 @default.
• W3157553013 creator A5089677448 @default.
• W3157553013 date "2021-04-27" @default.
• W3157553013 modified "2023-10-18" @default.
• W3157553013 title "Test-Retest Reliability of Flow Cytometric Quantification of Circulating Endothelial Cells and Endothelial Progenitor Cells in Cardiovascular Diseases" @default.
• W3157553013 cites W1907650756 @default.
• W3157553013 cites W1970909475 @default.
• W3157553013 cites W1983541079 @default.
• W3157553013 cites W1993245079 @default.
• W3157553013 cites W2002922221 @default.
• W3157553013 cites W2005156700 @default.
• W3157553013 cites W2015795623 @default.
• W3157553013 cites W2021272479 @default.
• W3157553013 cites W2024081693 @default.
• W3157553013 cites W2024263079 @default.
• W3157553013 cites W2025299174 @default.
• W3157553013 cites W2025806325 @default.
• W3157553013 cites W2035675650 @default.
• W3157553013 cites W2067744701 @default.
• W3157553013 cites W2068994530 @default.
• W3157553013 cites W2072181292 @default.
• W3157553013 cites W2085350659 @default.
• W3157553013 cites W2090131524 @default.
• W3157553013 cites W2097393771 @default.
• W3157553013 cites W2109680555 @default.
• W3157553013 cites W2113617288 @default.
• W3157553013 cites W2127039247 @default.
• W3157553013 cites W2127872440 @default.
• W3157553013 cites W2134388588 @default.
• W3157553013 cites W2135165880 @default.
• W3157553013 cites W2141403362 @default.
• W3157553013 cites W2164177263 @default.
• W3157553013 cites W2167184179 @default.
• W3157553013 cites W2171284332 @default.
• W3157553013 cites W2327037637 @default.
• W3157553013 cites W2340327599 @default.
• W3157553013 cites W2618268791 @default.
• W3157553013 cites W2644819006 @default.
• W3157553013 cites W2796822136 @default.
• W3157553013 cites W2898565675 @default.
• W3157553013 cites W2912349604 @default.
• W3157553013 cites W590111097 @default.
• W3157553013 hasPublicationYear "2021" @default.
• W3157553013 type Work @default.
• W3157553013 sameAs 3157553013 @default.
• W3157553013 citedByCount "0" @default.
• W3157553013 crossrefType "journal-article" @default.
• W3157553013 hasAuthorship W3157553013A5017858830 @default.
• W3157553013 hasAuthorship W3157553013A5042631889 @default.
• W3157553013 hasAuthorship W3157553013A5045374209 @default.
• W3157553013 hasAuthorship W3157553013A5049347390 @default.
• W3157553013 hasAuthorship W3157553013A5069025004 @default.
• W3157553013 hasAuthorship W3157553013A5073076418 @default.
• W3157553013 hasAuthorship W3157553013A5075618911 @default.
• W3157553013 hasAuthorship W3157553013A5079924425 @default.
• W3157553013 hasAuthorship W3157553013A5082519884 @default.
• W3157553013 hasAuthorship W3157553013A5086287347 @default.
• W3157553013 hasAuthorship W3157553013A5089677448 @default.
• W3157553013 hasConcept C10205521 @default.
• W3157553013 hasConcept C104709138 @default.
• W3157553013 hasConcept C126322002 @default.
• W3157553013 hasConcept C164705383 @default.
• W3157553013 hasConcept C171606756 @default.
• W3157553013 hasConcept C201750760 @default.
• W3157553013 hasConcept C2778198053 @default.
• W3157553013 hasConcept C2778939058 @default.
• W3157553013 hasConcept C2779093498 @default.
• W3157553013 hasConcept C2780152582 @default.
• W3157553013 hasConcept C2780394083 @default.
• W3157553013 hasConcept C2780972559 @default.
• W3157553013 hasConcept C28328180 @default.
• W3157553013 hasConcept C54355233 @default.
• W3157553013 hasConcept C70410870 @default.
• W3157553013 hasConcept C71924100 @default.
• W3157553013 hasConcept C78085059 @default.
• W3157553013 hasConcept C86803240 @default.
• W3157553013 hasConceptScore W3157553013C10205521 @default.
• W3157553013 hasConceptScore W3157553013C104709138 @default.
• W3157553013 hasConceptScore W3157553013C126322002 @default.
• W3157553013 hasConceptScore W3157553013C164705383 @default.
• W3157553013 hasConceptScore W3157553013C171606756 @default.
• W3157553013 hasConceptScore W3157553013C201750760 @default.
• W3157553013 hasConceptScore W3157553013C2778198053 @default.
• W3157553013 hasConceptScore W3157553013C2778939058 @default.
• W3157553013 hasConceptScore W3157553013C2779093498 @default.
• W3157553013 hasConceptScore W3157553013C2780152582 @default.
• W3157553013 hasConceptScore W3157553013C2780394083 @default.

• next