Matches in SemOpenAlex for { <https://semopenalex.org/work/W3157617479> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W3157617479 endingPage "114220" @default.
- W3157617479 startingPage "114220" @default.
- W3157617479 abstract "In this paper, we present the applications of our newly developed, highly sensitive fluorescent detection method referred to as scanning single-molecule counting (SSMC). We found that the target RNA added to the total RNA was detected with high sensitivity at 384 aM by combining a magnetic bead-based assay and SSMC (MB-BA + SSMC). Gene expression analysis without reverse transcription or amplification confirmed that the pattern of gene expression was identical to that of real-time polymerase chain reaction (PCR). MB-BA + SSMC was also applied to formalin-fixed paraffin-embedded (FFPE) samples. RNA fragmentation and crosslinking owing to FFPE processing slightly affected gene expression. Conversely, FFPE samples showed an increase in gene Ct values and a decrease in the number of detectable genes when analyzed using real-time PCR. Overall, our results suggested that SSMC is a powerful tool for target RNA detection and amplification-free gene expression analysis. • SSMC enables highly sensitive RNA detection without amplification. • FFPE samples show a decreased number of detectable genes on real-time PCR. • RNA damage owing to FFPE processing slightly affect gene expression using SSMC." @default.
- W3157617479 created "2021-05-10" @default.
- W3157617479 creator A5016395681 @default.
- W3157617479 creator A5037446549 @default.
- W3157617479 creator A5048741157 @default.
- W3157617479 creator A5050010936 @default.
- W3157617479 creator A5055815911 @default.
- W3157617479 date "2021-07-01" @default.
- W3157617479 modified "2023-09-24" @default.
- W3157617479 title "Amplification-free gene expression analysis of formalin-fixed paraffin-embedded samples using scanning single-molecule counting" @default.
- W3157617479 cites W1986077582 @default.
- W3157617479 cites W1992425156 @default.
- W3157617479 cites W2033620247 @default.
- W3157617479 cites W2122749842 @default.
- W3157617479 cites W2126265192 @default.
- W3157617479 cites W2136875315 @default.
- W3157617479 cites W2145684092 @default.
- W3157617479 cites W3111762078 @default.
- W3157617479 doi "https://doi.org/10.1016/j.ab.2021.114220" @default.
- W3157617479 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33915118" @default.
- W3157617479 hasPublicationYear "2021" @default.
- W3157617479 type Work @default.
- W3157617479 sameAs 3157617479 @default.
- W3157617479 citedByCount "0" @default.
- W3157617479 crossrefType "journal-article" @default.
- W3157617479 hasAuthorship W3157617479A5016395681 @default.
- W3157617479 hasAuthorship W3157617479A5037446549 @default.
- W3157617479 hasAuthorship W3157617479A5048741157 @default.
- W3157617479 hasAuthorship W3157617479A5050010936 @default.
- W3157617479 hasAuthorship W3157617479A5055815911 @default.
- W3157617479 hasConcept C104317684 @default.
- W3157617479 hasConcept C150194340 @default.
- W3157617479 hasConcept C153911025 @default.
- W3157617479 hasConcept C48023723 @default.
- W3157617479 hasConcept C49105822 @default.
- W3157617479 hasConcept C54355233 @default.
- W3157617479 hasConcept C67705224 @default.
- W3157617479 hasConcept C86803240 @default.
- W3157617479 hasConceptScore W3157617479C104317684 @default.
- W3157617479 hasConceptScore W3157617479C150194340 @default.
- W3157617479 hasConceptScore W3157617479C153911025 @default.
- W3157617479 hasConceptScore W3157617479C48023723 @default.
- W3157617479 hasConceptScore W3157617479C49105822 @default.
- W3157617479 hasConceptScore W3157617479C54355233 @default.
- W3157617479 hasConceptScore W3157617479C67705224 @default.
- W3157617479 hasConceptScore W3157617479C86803240 @default.
- W3157617479 hasFunder F4320311497 @default.
- W3157617479 hasLocation W31576174791 @default.
- W3157617479 hasOpenAccess W3157617479 @default.
- W3157617479 hasPrimaryLocation W31576174791 @default.
- W3157617479 hasRelatedWork W1811249054 @default.
- W3157617479 hasRelatedWork W1993669460 @default.
- W3157617479 hasRelatedWork W2009966535 @default.
- W3157617479 hasRelatedWork W2056172754 @default.
- W3157617479 hasRelatedWork W2085668533 @default.
- W3157617479 hasRelatedWork W2115220490 @default.
- W3157617479 hasRelatedWork W2145879504 @default.
- W3157617479 hasRelatedWork W2754703032 @default.
- W3157617479 hasRelatedWork W2996742744 @default.
- W3157617479 hasRelatedWork W3133741315 @default.
- W3157617479 hasVolume "625" @default.
- W3157617479 isParatext "false" @default.
- W3157617479 isRetracted "false" @default.
- W3157617479 magId "3157617479" @default.
- W3157617479 workType "article" @default.