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• W3157841933 abstract "Streptococcus suis , particularly S . suis serotype 2 (SS2), is an important zoonotic pathogen causing meningitis in humans worldwide. Although the proper classification of the causative and pathogenic serotype is salutary for the clinical diagnosis, cross-reactions leading to the indistinguishability of serotypes by the current serotyping methods are significant limitations. In the present study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis of extracted peptides was developed to improve the classification of serotype of S . suis . The peptide mass fingerprint (PMFs) database of S . suis was generated from the whole-cell peptides of 32 reference strains of S . suis isolates obtained from pigs. Thirty-two human S . suis isolates from clinical cases in Thailand were used to validate this alternative serotyping method in direct comparison to the multiplex (m)PCR approach. All reference strains, representing 32 serotypes of S . suis , exhibited their individual PMFs patterns, thus allowing differentiation from one another. Highly pathogenic SS2 and SS14 were clearly differentiated from the otherwise serologically closely related SS1/2 and SS1, respectively. The developed MALDI-TOF-MS serotyping method correctly classified the serotype in 68.8% (22/32) of the same serotype isolates generated from the PMFs database; while the validity for the clinical human isolates was 62.5% (20/32). The agreement between the MALDI-TOF-MS and mPCR serotyping was moderate with a Kappa score of 0.522, considering that mPCR could correctly serotype up to 75%. The present study demonstrated that PMFs from the developed MALDI-TOF-MS-based method could successfully discriminate the previously indistinguishable highly pathogenic SS2 and SS14 from SS1/2 and SS1, respectively. Moreover, this serotyping method distinguished pathogenic SS6, and so is an alternative approach of choice to rapidly and reliably serotype clinically pathogenic S . suis isolates." @default.
• W3157841933 created "2021-05-10" @default.
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• W3157841933 date "2021-05-04" @default.
• W3157841933 modified "2023-10-18" @default.
• W3157841933 title "Streptococcus suis serotyping by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry" @default.
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• W3157841933 doi "https://doi.org/10.1371/journal.pone.0249682" @default.
• W3157841933 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8096114" @default.
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