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- W3157934002 abstract "Apolipoprotein E (ApoE), an important mediator of lipid transportation in plasma and the nervous system, plays a large role in diseases such as atherosclerosis and Alzheimer's. The major allele variants ApoE3 and ApoE4 differ only by one amino acid. However, this difference has major consequences for the physiological behaviour of each variant. In this paper, we follow (i) the initial interaction of lipid-free ApoE variants with model membranes as a function of lipid saturation, (ii) the formation of reconstituted High-Density Lipoprotein-like particles (rHDL) and their structural characterisation, and (iii) the rHDL ability to exchange lipids with model membranes made of saturated lipids in the presence and absence of cholesterol [1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) or 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) with and without 20 mol% cholesterol]. Our neutron reflection results demonstrate that the protein variants interact differently with the model membranes, adopting different protein conformations. Moreover, the ApoE3 structure at the model membrane is sensitive to the level of lipid unsaturation. Small-angle neutron scattering shows that the ApoE containing lipid particles form elliptical disc-like structures, similar in shape but larger than nascent or discoidal HDL based on Apolipoprotein A1 (ApoA1). Neutron reflection shows that ApoE-rHDL do not remove cholesterol but rather exchange saturated lipids, as occurs in the brain. In contrast, ApoA1-containing particles remove and exchange lipids to a greater extent as occurs elsewhere in the body." @default.
- W3157934002 created "2021-05-10" @default.
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- W3157934002 date "2021-04-29" @default.
- W3157934002 modified "2023-10-18" @default.
- W3157934002 title "ApoE and ApoE Nascent-Like HDL Particles at Model Cellular Membranes: Effect of Protein Isoform and Membrane Composition" @default.
- W3157934002 cites W151905713 @default.
- W3157934002 cites W1546262989 @default.
- W3157934002 cites W1856419029 @default.
- W3157934002 cites W1899059318 @default.
- W3157934002 cites W1963021413 @default.
- W3157934002 cites W1976561133 @default.
- W3157934002 cites W1976725721 @default.
- W3157934002 cites W1977728730 @default.
- W3157934002 cites W1988748406 @default.
- W3157934002 cites W1990016625 @default.
- W3157934002 cites W1994197757 @default.
- W3157934002 cites W1994509415 @default.
- W3157934002 cites W1997394451 @default.
- W3157934002 cites W1997765587 @default.
- W3157934002 cites W2004899462 @default.
- W3157934002 cites W2005574282 @default.
- W3157934002 cites W2006599070 @default.
- W3157934002 cites W2008062676 @default.
- W3157934002 cites W2008937801 @default.
- W3157934002 cites W2012859837 @default.
- W3157934002 cites W2017041065 @default.
- W3157934002 cites W2020581548 @default.
- W3157934002 cites W2021265420 @default.
- W3157934002 cites W2021876176 @default.
- W3157934002 cites W2024616958 @default.
- W3157934002 cites W2033637550 @default.
- W3157934002 cites W2037063580 @default.
- W3157934002 cites W2038672689 @default.
- W3157934002 cites W2042712943 @default.
- W3157934002 cites W2043090568 @default.
- W3157934002 cites W2044097830 @default.
- W3157934002 cites W2047397116 @default.
- W3157934002 cites W2049660003 @default.
- W3157934002 cites W2051108089 @default.
- W3157934002 cites W2051415561 @default.
- W3157934002 cites W2053525596 @default.
- W3157934002 cites W2056451248 @default.
- W3157934002 cites W2064910975 @default.
- W3157934002 cites W2067160010 @default.
- W3157934002 cites W2071475257 @default.
- W3157934002 cites W2077760013 @default.
- W3157934002 cites W2087083058 @default.
- W3157934002 cites W2089331480 @default.
- W3157934002 cites W2092706511 @default.
- W3157934002 cites W2101101778 @default.
- W3157934002 cites W2106601510 @default.
- W3157934002 cites W2107887632 @default.
- W3157934002 cites W2108198268 @default.
- W3157934002 cites W2121774886 @default.
- W3157934002 cites W2128633067 @default.
- W3157934002 cites W2136203025 @default.
- W3157934002 cites W2141623310 @default.
- W3157934002 cites W2143294881 @default.
- W3157934002 cites W2143416920 @default.
- W3157934002 cites W2145813488 @default.
- W3157934002 cites W2147492435 @default.
- W3157934002 cites W2153350589 @default.
- W3157934002 cites W2201824626 @default.
- W3157934002 cites W2205934616 @default.
- W3157934002 cites W2232236906 @default.
- W3157934002 cites W2237782413 @default.
- W3157934002 cites W2313768139 @default.
- W3157934002 cites W2316885128 @default.
- W3157934002 cites W2325931377 @default.
- W3157934002 cites W2416854880 @default.
- W3157934002 cites W2560393860 @default.
- W3157934002 cites W2574219503 @default.
- W3157934002 cites W2600991905 @default.
- W3157934002 cites W2606324234 @default.
- W3157934002 cites W2741302226 @default.
- W3157934002 cites W2751593999 @default.
- W3157934002 cites W2764065379 @default.
- W3157934002 cites W2773867636 @default.
- W3157934002 cites W2790424003 @default.
- W3157934002 cites W2791422845 @default.
- W3157934002 cites W2894616244 @default.
- W3157934002 cites W2923775575 @default.
- W3157934002 cites W2945846066 @default.
- W3157934002 cites W3003265699 @default.