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- W3158275928 abstract "Microalgae-derived carotenoids have increasingly been considered as feasible green alternatives for synthetic antioxidants. In this study, the lutein high-yielding strain (Chlorella sorokiniana MB-1; henceforth MB-1) and its mutant derivative (C. sorokiniana MB-1-M12; henceforth M12) were evaluated for their growth, biomass production, and lutein accumulation in three different cultivation modes – photoautotrophy, mixotrophy, and heterotrophy. M12 could grow effectively under heterotrophic conditions, but the lutein content was lower, indicating the necessity of photo-induction for lutein accumulation. Metabolic analysis of MB-1 and M12 in autotrophic growth in the presence of carbon dioxide indicated that carbon assimilation and channeling of the fixed metabolites towards carotenoid accumulation was elevated in M12 compared to MB-1. Novel two-stage alternative cultivation strategies (Autotrophic/Heterotrophic and Mixotrophic/Heterotrophic cultures) were applied for enhancing lutein production in M12. Maximum lutein quantity (6.17 mg/g) and production (33.64 mg/L) were obtained with the TSHM strategy that is considered the best two-stage operation." @default.
- W3158275928 created "2021-05-10" @default.
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- W3158275928 date "2021-08-01" @default.
- W3158275928 modified "2023-10-13" @default.
- W3158275928 title "Lutein production with Chlorella sorokiniana MB-1-M12 using novel two-stage cultivation strategies – metabolic analysis and process improvement" @default.
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- W3158275928 doi "https://doi.org/10.1016/j.biortech.2021.125200" @default.
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