FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3158446915> ?p ?o ?g. }

• W3158446915 endingPage "A807" @default.
• W3158446915 startingPage "A806" @default.
• W3158446915 abstract "Abstract Introduction: Chronic glucocorticoid administration is necessary in a variety of conditions including but not limited to autoimmune, inflammatory and cancer-related diseases in order to relieve symptoms and sustain disease progression. However, there are adverse effects that include increase in glucose levels and others whose severity depends on the dose and duration of glucocorticoid exposure. It has been described that dexamethasone induces oxidative stress in cells by increasing reactive oxygen species (ROS) and this is one of the causes of insulin resistance at the cellular level. Nrf2 is a transcription factor which co-ordinates the antioxidant response and its activation has been shown to ameliorate insulin resistance in murine models. Hypothesis: We hypothesized that deletion of Nrf2 will lead to a more glucose intolerant insulin resistant phenotype in mice chronically treated with dexamethasone as cells would be exposed to higher ROS levels. Methods: To this end, 3-months old wild-type (WT) and Nrf2 knockout (KO) C57BL6J mice were treated intraperitoneally with 2 mg/kg dexamethasone or saline 3 times per week for 3 months. 5-10 mice were included per genotype per treatment and both male and female mice were used. Weekly measurements of body weights were performed and intraperitoneal glucose tolerance tests were done on the second and third month of treatment. Mice were sacrificed 24 hours after the last dose of dexamethasone and blood, white adipose tissue, soleus muscle and liver were collected for RNA preparation and quantitative RT-PCR analysis. Quantitative analysis of trabecular bone parameters was performed by micro-CT. Results: Both male and female mice treated with dexamethasone gained less weight over time and surprisingly were more glucose tolerant than the control group. Absence of Nrf2 did not seem to considerably affect the body weight but KO mice tended to have lower body weights after dexamethasone treatment in both genders with the effect on male mice being statistically significant (25% lower, p&lt;0.05). Surprisingly, both WT and KO mice of both genders showed lower fasting blood glucose levels after 3 months of treatment and better glucose tolerance. Livers of KO mice showed lower levels (~50%) of the cytoprotective genes Nqo1 and Gclc as expected but no difference was observed after dexamethasone treatment. Sarcopenia muscle markers Mafbx1 and Murf1 showed no significant changes. Male mice showed increased expression of Pnpla3 in white adipose tissue indicating increased lipolysis upon dexamethasone exposure. Micro-CT showed minor changes in the bone parameters without difference between male WT and Nrf2KO mice. Conclusions: Dexamethasone unexpectedly led to better glucose tolerance and lower body weight which is uncommon in humans but it has been described previously in mouse models. More analyses are in progress to fully elucidate this phenotype." @default.
• W3158446915 created "2021-05-10" @default.
• W3158446915 creator A5008629286 @default.
• W3158446915 creator A5012053115 @default.
• W3158446915 creator A5017001990 @default.
• W3158446915 creator A5029395816 @default.
• W3158446915 creator A5056745207 @default.
• W3158446915 date "2021-05-01" @default.
• W3158446915 modified "2023-09-27" @default.
• W3158446915 title "Chronic Dexamethasone Treatment Leads to Less Weight Gain and Ameliorated Glucose Tolerance in Mice; Role of the Cytoprotective Nrf2 Pathway" @default.
• W3158446915 doi "https://doi.org/10.1210/jendso/bvab048.1640" @default.
• W3158446915 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8089894" @default.
• W3158446915 hasPublicationYear "2021" @default.
• W3158446915 type Work @default.
• W3158446915 sameAs 3158446915 @default.
• W3158446915 citedByCount "1" @default.
• W3158446915 countsByYear W31584469152022 @default.
• W3158446915 crossrefType "journal-article" @default.
• W3158446915 hasAuthorship W3158446915A5008629286 @default.
• W3158446915 hasAuthorship W3158446915A5012053115 @default.
• W3158446915 hasAuthorship W3158446915A5017001990 @default.
• W3158446915 hasAuthorship W3158446915A5029395816 @default.
• W3158446915 hasAuthorship W3158446915A5056745207 @default.
• W3158446915 hasBestOaLocation W31584469151 @default.
• W3158446915 hasConcept C126322002 @default.
• W3158446915 hasConcept C134018914 @default.
• W3158446915 hasConcept C151955695 @default.
• W3158446915 hasConcept C171089720 @default.
• W3158446915 hasConcept C2776151105 @default.
• W3158446915 hasConcept C2776804153 @default.
• W3158446915 hasConcept C2777391703 @default.
• W3158446915 hasConcept C2779113265 @default.
• W3158446915 hasConcept C2779306644 @default.
• W3158446915 hasConcept C2780401358 @default.
• W3158446915 hasConcept C2780841215 @default.
• W3158446915 hasConcept C3017594256 @default.
• W3158446915 hasConcept C71924100 @default.
• W3158446915 hasConceptScore W3158446915C126322002 @default.
• W3158446915 hasConceptScore W3158446915C134018914 @default.
• W3158446915 hasConceptScore W3158446915C151955695 @default.
• W3158446915 hasConceptScore W3158446915C171089720 @default.
• W3158446915 hasConceptScore W3158446915C2776151105 @default.
• W3158446915 hasConceptScore W3158446915C2776804153 @default.
• W3158446915 hasConceptScore W3158446915C2777391703 @default.
• W3158446915 hasConceptScore W3158446915C2779113265 @default.
• W3158446915 hasConceptScore W3158446915C2779306644 @default.
• W3158446915 hasConceptScore W3158446915C2780401358 @default.
• W3158446915 hasConceptScore W3158446915C2780841215 @default.
• W3158446915 hasConceptScore W3158446915C3017594256 @default.
• W3158446915 hasConceptScore W3158446915C71924100 @default.
• W3158446915 hasIssue "Supplement_1" @default.
• W3158446915 hasLocation W31584469151 @default.
• W3158446915 hasLocation W31584469152 @default.
• W3158446915 hasOpenAccess W3158446915 @default.
• W3158446915 hasPrimaryLocation W31584469151 @default.
• W3158446915 hasRelatedWork W1957106181 @default.
• W3158446915 hasRelatedWork W2010484394 @default.
• W3158446915 hasRelatedWork W2018372630 @default.
• W3158446915 hasRelatedWork W2032040801 @default.
• W3158446915 hasRelatedWork W2037265213 @default.
• W3158446915 hasRelatedWork W2053534902 @default.
• W3158446915 hasRelatedWork W2076356898 @default.
• W3158446915 hasRelatedWork W2079804504 @default.
• W3158446915 hasRelatedWork W2113795181 @default.
• W3158446915 hasRelatedWork W2146053648 @default.
• W3158446915 hasVolume "5" @default.
• W3158446915 isParatext "false" @default.
• W3158446915 isRetracted "false" @default.
• W3158446915 magId "3158446915" @default.
• W3158446915 workType "article" @default.