FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3158606990> ?p ?o ?g. }

• W3158606990 abstract "Heparin, a widely used antithrombotic drug has many other anticoagulant-independent physiological functions. Here, we elucidate a novel role of heparin in glucose homeostasis, suggesting an approach for developing heparin-targeted therapies for diabetes.For serum heparin levels and correlation analysis, 122 volunteer's plasma, DIO (4 weeks HFD) and db/db mice serums were collected and used for spectrophotometric determination. OGTT, ITT, 2-NBDG uptake and muscle GLUT4 immunofluorescence were detected in chronic intraperitoneal injection of heparin or heparinase (16 days) and muscle-specific loss-of-function mice. In 293T cells, the binding of insulin to its receptor was detected by fluorescence resonance energy transfer (FRET), Myc-GLUT4-mCherry plasmid was used in GLUT4 translocation. In vitro, C2C12 cells as mouse myoblast cells were further verified the effects of heparin on glucose homeostasis through 2-NBDG uptake, Western blot and co-immunoprecipitation.Serum concentrations of heparin are positively associated with blood glucose levels in humans and are significantly increased in diet-induced and db/db obesity mouse models. Consistently, a chronic intraperitoneal injection of heparin results in hyperglycaemia, glucose intolerance and insulin resistance. These effects are independent of heparin's anticoagulant function and associated with decreases in glucose uptake and translocation of glucose transporter type 4 (GLUT4) in skeletal muscle. By using a muscle-specific loss-of-function mouse model, we further demonstrated that muscle GLUT4 is required for the detrimental effects of heparin on glucose homeostasis.Heparin reduced insulin binding to its receptor by interacting with insulin and inhibited insulin-mediated activation of the PI3K/Akt signalling pathway in skeletal muscle, which leads to impaired glucose uptake and hyperglycaemia." @default.
• W3158606990 created "2021-05-10" @default.
• W3158606990 creator A5008259702 @default.
• W3158606990 creator A5009217135 @default.
• W3158606990 creator A5011740024 @default.
• W3158606990 creator A5014113137 @default.
• W3158606990 creator A5019256562 @default.
• W3158606990 creator A5026929036 @default.
• W3158606990 creator A5029293216 @default.
• W3158606990 creator A5034628620 @default.
• W3158606990 creator A5042947574 @default.
• W3158606990 creator A5049845476 @default.
• W3158606990 creator A5052979720 @default.
• W3158606990 creator A5059293774 @default.
• W3158606990 creator A5066896791 @default.
• W3158606990 creator A5067483315 @default.
• W3158606990 creator A5072674243 @default.
• W3158606990 creator A5073218187 @default.
• W3158606990 creator A5085142676 @default.
• W3158606990 creator A5091160749 @default.
• W3158606990 date "2021-05-05" @default.
• W3158606990 modified "2023-10-17" @default.
• W3158606990 title "Heparin impairs skeletal muscle glucose uptake by inhibiting insulin binding to insulin receptor" @default.
• W3158606990 cites W1498644559 @default.
• W3158606990 cites W1578864848 @default.
• W3158606990 cites W1580646470 @default.
• W3158606990 cites W1582656672 @default.
• W3158606990 cites W1947673554 @default.
• W3158606990 cites W1971041352 @default.
• W3158606990 cites W1971857747 @default.
• W3158606990 cites W1972312145 @default.
• W3158606990 cites W1972476556 @default.
• W3158606990 cites W1978103932 @default.
• W3158606990 cites W1993456360 @default.
• W3158606990 cites W1995281180 @default.
• W3158606990 cites W2004889053 @default.
• W3158606990 cites W2010386176 @default.
• W3158606990 cites W2011717731 @default.
• W3158606990 cites W2016235535 @default.
• W3158606990 cites W2024682108 @default.
• W3158606990 cites W2025323849 @default.
• W3158606990 cites W2030137409 @default.
• W3158606990 cites W2045707415 @default.
• W3158606990 cites W2048565142 @default.
• W3158606990 cites W2053127486 @default.
• W3158606990 cites W2060565509 @default.
• W3158606990 cites W2062244719 @default.
• W3158606990 cites W2070089423 @default.
• W3158606990 cites W2077799214 @default.
• W3158606990 cites W2091356803 @default.
• W3158606990 cites W2100304409 @default.
• W3158606990 cites W2102214196 @default.
• W3158606990 cites W2117151621 @default.
• W3158606990 cites W2133706171 @default.
• W3158606990 cites W2138521217 @default.
• W3158606990 cites W2145380240 @default.
• W3158606990 cites W2147863229 @default.
• W3158606990 cites W2156052955 @default.
• W3158606990 cites W2158413166 @default.
• W3158606990 cites W2161976814 @default.
• W3158606990 cites W2164006717 @default.
• W3158606990 cites W2166990204 @default.
• W3158606990 cites W2170869159 @default.
• W3158606990 cites W2203460689 @default.
• W3158606990 cites W2264105514 @default.
• W3158606990 cites W2401654102 @default.
• W3158606990 cites W2417061871 @default.
• W3158606990 cites W2417808663 @default.
• W3158606990 cites W2515236049 @default.
• W3158606990 cites W2528876883 @default.
• W3158606990 cites W2532158419 @default.
• W3158606990 cites W2557927211 @default.
• W3158606990 cites W2590054727 @default.
• W3158606990 cites W2612882275 @default.
• W3158606990 cites W2752728229 @default.
• W3158606990 cites W2759256139 @default.
• W3158606990 cites W2766310642 @default.
• W3158606990 cites W2888464263 @default.
• W3158606990 cites W2921092881 @default.
• W3158606990 cites W2963697512 @default.
• W3158606990 cites W4230185659 @default.
• W3158606990 doi "https://doi.org/10.1002/edm2.253" @default.
• W3158606990 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8279624" @default.
• W3158606990 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34277977" @default.
• W3158606990 hasPublicationYear "2021" @default.
• W3158606990 type Work @default.
• W3158606990 sameAs 3158606990 @default.
• W3158606990 citedByCount "2" @default.
• W3158606990 countsByYear W31586069902022 @default.
• W3158606990 countsByYear W31586069902023 @default.
• W3158606990 crossrefType "journal-article" @default.
• W3158606990 hasAuthorship W3158606990A5008259702 @default.
• W3158606990 hasAuthorship W3158606990A5009217135 @default.
• W3158606990 hasAuthorship W3158606990A5011740024 @default.
• W3158606990 hasAuthorship W3158606990A5014113137 @default.
• W3158606990 hasAuthorship W3158606990A5019256562 @default.
• W3158606990 hasAuthorship W3158606990A5026929036 @default.
• W3158606990 hasAuthorship W3158606990A5029293216 @default.
• W3158606990 hasAuthorship W3158606990A5034628620 @default.
• W3158606990 hasAuthorship W3158606990A5042947574 @default.

• next