FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3158743880> ?p ?o ?g. }

• W3158743880 abstract "Abstract Background : Nano-sized exosomes (30–150 nm) are cell membrane-encapsulated vesicles that contain nucleic acids and proteins. Specific markers detecting non-small cell lung cancer (NSCLC) cell-derived exosomes in the blood circulation remain unidentified. Here, we report a new biomarker distinguishing cancer from non-cancer exosomes that also involved in epithelial to mesenchymal transition for cancer treatment. Methods : Exosomes were isolated from plasma of patients with various pathological stages of NSCLC and NSCLC cell lines, human pulmonary alveolar epithelial cells by size exclusion chromatography and characterized by Nanoparticle Tracking Analysis and western-blotting. The exosomes were lysed and applied to proteomic analysis. The expression levels of the GCC2 proteins from NSCLC patients were analyzed by ELISA assays, and the effects by GCC2 shRNA were analyzed by real-time RT-PCR, cell migration and colony formation assays. Results : A protein GRIP and coiled-coil domain-containing 2 (GCC2), which is involved in endosome-to-Golgi transport, was identified by the proteomics analysis of exosomes isolated from NSCLC cell lines. The GCC2 protein expression levels were increased in the exosomes derived from patients with early-stage NSCLC compared with healthy controls. The receiver operating characteristic curve of exosomal GCC2 revealed 94.74% sensitivity and 75.00% specificity, and AUC of 0.875. GCC2 knockdown experiments by GCC2 shRNA showed reduced exosome secretion in cancer cell lines, which altered the molecular and cellular properties, such as the expression levels of mesenchymal-to-epithelial genes, and cellular growth and motility. Conclusion : GCC2 represents a promising biomarker for early diagnosis of NSCLC and a therapeutic target for future cancer treatment." @default.
• W3158743880 created "2021-05-10" @default.
• W3158743880 creator A5000217772 @default.
• W3158743880 creator A5008787917 @default.
• W3158743880 creator A5009814376 @default.
• W3158743880 creator A5013188367 @default.
• W3158743880 creator A5017536228 @default.
• W3158743880 creator A5023527480 @default.
• W3158743880 creator A5024472979 @default.
• W3158743880 creator A5031634045 @default.
• W3158743880 creator A5032093141 @default.
• W3158743880 creator A5038016384 @default.
• W3158743880 creator A5059431081 @default.
• W3158743880 creator A5073611697 @default.
• W3158743880 creator A5087234700 @default.
• W3158743880 date "2020-12-29" @default.
• W3158743880 modified "2023-09-26" @default.
• W3158743880 title "GCC2 is a New Biomarker for Diagnosis of Early Non-Small Cell Lung Cancer and A Potential Target to Reverse Epithelial to Mesenchymal Transition" @default.
• W3158743880 doi "https://doi.org/10.21203/rs.3.rs-135611/v1" @default.
• W3158743880 hasPublicationYear "2020" @default.
• W3158743880 type Work @default.
• W3158743880 sameAs 3158743880 @default.
• W3158743880 citedByCount "0" @default.
• W3158743880 crossrefType "posted-content" @default.
• W3158743880 hasAuthorship W3158743880A5000217772 @default.
• W3158743880 hasAuthorship W3158743880A5008787917 @default.
• W3158743880 hasAuthorship W3158743880A5009814376 @default.
• W3158743880 hasAuthorship W3158743880A5013188367 @default.
• W3158743880 hasAuthorship W3158743880A5017536228 @default.
• W3158743880 hasAuthorship W3158743880A5023527480 @default.
• W3158743880 hasAuthorship W3158743880A5024472979 @default.
• W3158743880 hasAuthorship W3158743880A5031634045 @default.
• W3158743880 hasAuthorship W3158743880A5032093141 @default.
• W3158743880 hasAuthorship W3158743880A5038016384 @default.
• W3158743880 hasAuthorship W3158743880A5059431081 @default.
• W3158743880 hasAuthorship W3158743880A5073611697 @default.
• W3158743880 hasAuthorship W3158743880A5087234700 @default.
• W3158743880 hasBestOaLocation W31587438801 @default.
• W3158743880 hasConcept C104317684 @default.
• W3158743880 hasConcept C121608353 @default.
• W3158743880 hasConcept C142724271 @default.
• W3158743880 hasConcept C145059251 @default.
• W3158743880 hasConcept C1491633281 @default.
• W3158743880 hasConcept C153911025 @default.
• W3158743880 hasConcept C173396325 @default.
• W3158743880 hasConcept C185592680 @default.
• W3158743880 hasConcept C190232843 @default.
• W3158743880 hasConcept C198826908 @default.
• W3158743880 hasConcept C20518536 @default.
• W3158743880 hasConcept C2776256026 @default.
• W3158743880 hasConcept C2778120352 @default.
• W3158743880 hasConcept C2779013556 @default.
• W3158743880 hasConcept C2781197716 @default.
• W3158743880 hasConcept C2781261824 @default.
• W3158743880 hasConcept C502942594 @default.
• W3158743880 hasConcept C54355233 @default.
• W3158743880 hasConcept C55493867 @default.
• W3158743880 hasConcept C71924100 @default.
• W3158743880 hasConcept C76419328 @default.
• W3158743880 hasConcept C81885089 @default.
• W3158743880 hasConcept C86803240 @default.
• W3158743880 hasConcept C95444343 @default.
• W3158743880 hasConcept C96232424 @default.
• W3158743880 hasConceptScore W3158743880C104317684 @default.
• W3158743880 hasConceptScore W3158743880C121608353 @default.
• W3158743880 hasConceptScore W3158743880C142724271 @default.
• W3158743880 hasConceptScore W3158743880C145059251 @default.
• W3158743880 hasConceptScore W3158743880C1491633281 @default.
• W3158743880 hasConceptScore W3158743880C153911025 @default.
• W3158743880 hasConceptScore W3158743880C173396325 @default.
• W3158743880 hasConceptScore W3158743880C185592680 @default.
• W3158743880 hasConceptScore W3158743880C190232843 @default.
• W3158743880 hasConceptScore W3158743880C198826908 @default.
• W3158743880 hasConceptScore W3158743880C20518536 @default.
• W3158743880 hasConceptScore W3158743880C2776256026 @default.
• W3158743880 hasConceptScore W3158743880C2778120352 @default.
• W3158743880 hasConceptScore W3158743880C2779013556 @default.
• W3158743880 hasConceptScore W3158743880C2781197716 @default.
• W3158743880 hasConceptScore W3158743880C2781261824 @default.
• W3158743880 hasConceptScore W3158743880C502942594 @default.
• W3158743880 hasConceptScore W3158743880C54355233 @default.
• W3158743880 hasConceptScore W3158743880C55493867 @default.
• W3158743880 hasConceptScore W3158743880C71924100 @default.
• W3158743880 hasConceptScore W3158743880C76419328 @default.
• W3158743880 hasConceptScore W3158743880C81885089 @default.
• W3158743880 hasConceptScore W3158743880C86803240 @default.
• W3158743880 hasConceptScore W3158743880C95444343 @default.
• W3158743880 hasConceptScore W3158743880C96232424 @default.
• W3158743880 hasLocation W31587438801 @default.
• W3158743880 hasLocation W31587438802 @default.
• W3158743880 hasOpenAccess W3158743880 @default.
• W3158743880 hasPrimaryLocation W31587438801 @default.
• W3158743880 hasRelatedWork W2357051505 @default.
• W3158743880 hasRelatedWork W2571056385 @default.
• W3158743880 hasRelatedWork W2785527513 @default.
• W3158743880 hasRelatedWork W2944096190 @default.
• W3158743880 hasRelatedWork W2955875101 @default.
• W3158743880 hasRelatedWork W2968935972 @default.
• W3158743880 hasRelatedWork W3009964850 @default.
• W3158743880 hasRelatedWork W3198772284 @default.

• next