Matches in SemOpenAlex for { <https://semopenalex.org/work/W3158757480> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W3158757480 abstract "Acute respiratory distress syndrome (ARDS) is a life-threatening lung injury. After injury, severe inflammation, alveolar-capillary barrier permeability and alveolar epithelial cell (AEC) death lead to lung dysfunction. Glycogen synthase kinase-3 (GSK-3) is an important molecule in cell proliferation and differentiation. CHIR99021 (CHIR), a GSK-3 inhibitor, modulates the maturation of epithelial progenitor cells in lung 3D model. Thus, we aimed to understand the effects of CHIR on AEC regeneration using LPS-induced acute lung injury (ALI) mouse model. GSK-3 was inhibited 30min or 18h after LPS injection to investigate effects on disease progression in the early and late inflammatory response. Animals treated with CHIR for 30min were evaluated after 12h and 24h, and animals treated with CHIR for 18h were studied after 30h and 72h. We analyzed lung injury by H&E staining, barrier permeability by Evan Blue dye, inflammatory recruitment by flow cytometry, apoptosis by TUNEL and Cleaved Caspase-3 expression, proliferation by Cyclin D1 and PCNA expression. Moreover, the presence of AEC lineage was assessed by expression of HOPX (AECII-to-AECI differentiation), pro-SPC (AECII) and PDPN, AQP5 (AECI). GSK-3 inhibition reduced lung injury at 24 and 72h with no effect on inflammatory cell recruitment nor barrier permeability. CHIR enhanced cell proliferation at all times analyzed, increased AECII cell proliferation at 24h and bipotent cell proliferation at 24h and 72h. AECI-related molecules showed an increase at 24h, 30h and 72h and AECII-related markers were enhanced at 30h and 72h. In conclusion, our preliminary data suggest that CHIR promotes AEC proliferation and transdifferentiation, restores alveolar epithelial population, and thus promotes lung regeneration in LPS-induced ALI." @default.
- W3158757480 created "2021-05-10" @default.
- W3158757480 creator A5016509788 @default.
- W3158757480 creator A5030472020 @default.
- W3158757480 creator A5037726914 @default.
- W3158757480 creator A5060705806 @default.
- W3158757480 date "2021-03-11" @default.
- W3158757480 modified "2023-09-26" @default.
- W3158757480 title "Glycogen synthase kinase-3 inhibition by CHIR99021 promotes alveolar epithelial cell proliferation and lung regeneration in acute lung injury" @default.
- W3158757480 doi "https://doi.org/10.1183/23120541.lsc-2021.88" @default.
- W3158757480 hasPublicationYear "2021" @default.
- W3158757480 type Work @default.
- W3158757480 sameAs 3158757480 @default.
- W3158757480 citedByCount "1" @default.
- W3158757480 countsByYear W31587574802022 @default.
- W3158757480 crossrefType "proceedings-article" @default.
- W3158757480 hasAuthorship W3158757480A5016509788 @default.
- W3158757480 hasAuthorship W3158757480A5030472020 @default.
- W3158757480 hasAuthorship W3158757480A5037726914 @default.
- W3158757480 hasAuthorship W3158757480A5060705806 @default.
- W3158757480 hasConcept C126322002 @default.
- W3158757480 hasConcept C142724271 @default.
- W3158757480 hasConcept C182996813 @default.
- W3158757480 hasConcept C184235292 @default.
- W3158757480 hasConcept C185592680 @default.
- W3158757480 hasConcept C190283241 @default.
- W3158757480 hasConcept C196795494 @default.
- W3158757480 hasConcept C201750760 @default.
- W3158757480 hasConcept C203014093 @default.
- W3158757480 hasConcept C204232928 @default.
- W3158757480 hasConcept C2776914184 @default.
- W3158757480 hasConcept C2777714996 @default.
- W3158757480 hasConcept C28328180 @default.
- W3158757480 hasConcept C502942594 @default.
- W3158757480 hasConcept C55493867 @default.
- W3158757480 hasConcept C62112901 @default.
- W3158757480 hasConcept C71924100 @default.
- W3158757480 hasConcept C86803240 @default.
- W3158757480 hasConcept C95444343 @default.
- W3158757480 hasConceptScore W3158757480C126322002 @default.
- W3158757480 hasConceptScore W3158757480C142724271 @default.
- W3158757480 hasConceptScore W3158757480C182996813 @default.
- W3158757480 hasConceptScore W3158757480C184235292 @default.
- W3158757480 hasConceptScore W3158757480C185592680 @default.
- W3158757480 hasConceptScore W3158757480C190283241 @default.
- W3158757480 hasConceptScore W3158757480C196795494 @default.
- W3158757480 hasConceptScore W3158757480C201750760 @default.
- W3158757480 hasConceptScore W3158757480C203014093 @default.
- W3158757480 hasConceptScore W3158757480C204232928 @default.
- W3158757480 hasConceptScore W3158757480C2776914184 @default.
- W3158757480 hasConceptScore W3158757480C2777714996 @default.
- W3158757480 hasConceptScore W3158757480C28328180 @default.
- W3158757480 hasConceptScore W3158757480C502942594 @default.
- W3158757480 hasConceptScore W3158757480C55493867 @default.
- W3158757480 hasConceptScore W3158757480C62112901 @default.
- W3158757480 hasConceptScore W3158757480C71924100 @default.
- W3158757480 hasConceptScore W3158757480C86803240 @default.
- W3158757480 hasConceptScore W3158757480C95444343 @default.
- W3158757480 hasLocation W31587574801 @default.
- W3158757480 hasOpenAccess W3158757480 @default.
- W3158757480 hasPrimaryLocation W31587574801 @default.
- W3158757480 hasRelatedWork W2048403852 @default.
- W3158757480 hasRelatedWork W2347735562 @default.
- W3158757480 hasRelatedWork W2363045816 @default.
- W3158757480 hasRelatedWork W2371636476 @default.
- W3158757480 hasRelatedWork W2378500573 @default.
- W3158757480 hasRelatedWork W2378949748 @default.
- W3158757480 hasRelatedWork W2380078554 @default.
- W3158757480 hasRelatedWork W2388911714 @default.
- W3158757480 hasRelatedWork W2391900612 @default.
- W3158757480 hasRelatedWork W3176274664 @default.
- W3158757480 isParatext "false" @default.
- W3158757480 isRetracted "false" @default.
- W3158757480 magId "3158757480" @default.
- W3158757480 workType "article" @default.