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- W3159168032 abstract "Tissue-engineered replacement discs are an area of intense investigation for the treatment of end-stage intervertebral disc (IVD) degeneration. These living implants can integrate into the disc space and recapitulate native motion segment function. We recently developed a multiphasic tissue-engineered disc-like angle-ply structure (DAPS) that models the micro-architectural and functional features of native tissue. While these implants resulted in functional restoration of the motion segment in rat and caprine models, we also noted deficiencies in cell infiltration and homogeneity of matrix deposition in the electrospun poly(e-caprolactone) outer region (annulus fibrosus, AF) of the DAPS. To address this limitation, here, we incorporated a sacrificial water-soluble polymer, polyethylene oxide (PEO), as a second fiber fraction within the AF region to increase porosity of the implant. Maturation of these PEO-modified DAPS were evaluated after 5 and 10 weeks of in vitro culture in terms of AF biochemical content, MRI T2 values, overall construct mechanical properties, AF micromechanical properties and cell and matrix distribution. To assess the performance of the PEO-modified DAPS in vivo, precultured constructs were implanted into the rat caudal disc space for 10 weeks. Results showed that matrix distribution was more homogenous in PCL/PEO DAPS, as evidenced by more robust histological staining, organized collagen deposition and micromechanical properties, compared to standard PCL-only DAPS, both in vitro and in vivo. These PCL/PEO DAPS also better approximated native micro- and macro-mechanical properties than the PCL-only DAPS, following 10 weeks of in vivo implantation. These findings demonstrate that the inclusion of a sacrificial PEO fiber fraction in the DAPS AF region improves cellular colonization, matrix elaboration, and in vitro and in vivo function of an engineered disc implant." @default.
- W3159168032 created "2021-05-10" @default.
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- W3159168032 date "2020-01-01" @default.
- W3159168032 modified "2023-09-23" @default.
- W3159168032 title "Sacrificial Fibers Improve Matrix Distribution and Mechanical Properties in a Tissue-Engineered Intervertebral Disc" @default.
- W3159168032 doi "https://doi.org/10.2139/ssrn.3577260" @default.
- W3159168032 hasPublicationYear "2020" @default.
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