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• W3159193022 abstract "Edwardsiella piscicida is a leading pathogen that threats seawater and fresh aquaculture, in which the emergence and prevalence of bacterial resistance to antibiotics is a tricky problem. Currently, the pathogenesis of E. piscicida remains unclear, some new pathogenic factors need to be identified. Serine permease and dehydratase exist ubiquitously in microorganisms and participate in pathogenicity, but their functions in E. piscicida remain unknown. In this study, a serine permease YhaO and serine dehydratase YhaM were characterized in E. piscicida . yhaO Ep and yhaM Ep are expressed in the form of a bicistronic operon. YhaO Ep possesses multiple transmembrane structures and YhaM Ep is predicted to be located in the cytoplasm. To explore the biological roles of yhaO Ep and yhaM Ep , markerless in-frame mutant strains, TX01Δ yhaO and TX01Δ yhaM , were constructed, respectively. TX01, TX01Δ yhaO , and TX01Δ yhaM exhibited similar growth under normal and stress conditions, which indicates yhaO Ep and yhaM Ep are irrelevant to adversity. However, TX01Δ yhaO and TX01Δ yhaM displayed markedly reduced resistance against antibiotics, compared to TX01. Further studies indicated that the yhaO Ep mutation damaged E. piscicida 's ability to maintain membrane integrity and biofilm formation, decreased its adhesion and invasion to non-phagocytic FG cells, weakened the survival and proliferation in macrophage cells, and decreased bacterial dissemination in fish tissues. Complementary strain TX01Δ yhaO C restored these phenotypes of TX01Δ yhaO . The inactivation of yhaM Ep has no effect on bacterial pathogenicity to FG cells and macrophage cells, but in vivo experiment showed that TX01Δ yhaM exhibited attenuated capability of bacterial dissemination in fish tissues, compared to TX01. Taken together, our findings demonstrate for the first time that YhaO-YhaM is requisite for full pathogenicity of E. piscicida to host cells and host tissues, and provide new insights into the pathogenesis of E. piscicida . • yhaO Ep and yhaM Ep are irrelevant to response to adversity. • The deletion of yhaO Ep and yhaM Ep enhanced bacterial susceptibility to antibiotics. • yhaO Ep mutation damaged bacterial ability to maintain membrane integrity and biofilm formation. • yhaO Ep mutation decreased bacterial infectivity to non-phagocytes and phagocytes. • The deletion of yhaO Ep and yhaM Ep reduced bacterial pathogenicity to host tissues." @default.
• W3159193022 created "2021-05-10" @default.
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• W3159193022 date "2021-08-01" @default.
• W3159193022 modified "2023-09-30" @default.
• W3159193022 title "Bicistronic operon YhaO-YhaM contributes to antibiotic resistance and virulence of pathogen Edwardsiella piscicida" @default.
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• W3159193022 doi "https://doi.org/10.1016/j.aquaculture.2021.736849" @default.
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