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- W3159228535 abstract "The microalga Nannochloropsis oceanica is considered a promising platform for the sustainable production of high-value lipids and biofuel feedstocks. However, current lipid yields of N. oceanica are too low for economic feasibility. Gaining fundamental insights into the lipid metabolism of N. oceanica could open up various possibilities for the optimization of this species through genetic engineering. Therefore, the aim of this study was to discover novel genes associated with an elevated neutral lipid content. We constructed an insertional mutagenesis library of N. oceanica, selected high lipid mutants by five rounds of fluorescence-activated cell sorting, and identified disrupted genes using a novel implementation of a rapid genotyping procedure. One particularly promising mutant (HLM23) was disrupted in a putative APETALA2-like transcription factor gene. HLM23 showed a 40%-increased neutral lipid content, increased photosynthetic performance, and no growth impairment. Furthermore, transcriptome analysis revealed an upregulation of genes related to plastidial fatty acid biosynthesis, glycolysis and the Calvin-Benson-Bassham cycle in HLM23. Insights gained in this work can be used in future genetic engineering strategies for increased lipid productivity of Nannochloropsis." @default.
- W3159228535 created "2021-05-10" @default.
- W3159228535 creator A5003688889 @default.
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- W3159228535 creator A5074070508 @default.
- W3159228535 date "2021-07-01" @default.
- W3159228535 modified "2023-10-14" @default.
- W3159228535 title "High-throughput insertional mutagenesis reveals novel targets for enhancing lipid accumulation in Nannochloropsis oceanica" @default.
- W3159228535 cites W147428555 @default.
- W3159228535 cites W1633226001 @default.
- W3159228535 cites W1642400962 @default.
- W3159228535 cites W1963890672 @default.
- W3159228535 cites W1969304546 @default.
- W3159228535 cites W1970640134 @default.
- W3159228535 cites W1971089632 @default.
- W3159228535 cites W1971171842 @default.
- W3159228535 cites W1973670059 @default.
- W3159228535 cites W1982129514 @default.
- W3159228535 cites W1988506072 @default.
- W3159228535 cites W2000785460 @default.
- W3159228535 cites W2000991585 @default.
- W3159228535 cites W2004163437 @default.
- W3159228535 cites W2012011510 @default.
- W3159228535 cites W2013521084 @default.
- W3159228535 cites W2021429484 @default.
- W3159228535 cites W2023691395 @default.
- W3159228535 cites W2024591380 @default.
- W3159228535 cites W2025097672 @default.
- W3159228535 cites W2029355147 @default.
- W3159228535 cites W2034515048 @default.
- W3159228535 cites W2034908460 @default.
- W3159228535 cites W2036253883 @default.
- W3159228535 cites W2038186997 @default.
- W3159228535 cites W2042115934 @default.
- W3159228535 cites W2042412060 @default.
- W3159228535 cites W2054899605 @default.
- W3159228535 cites W2055995724 @default.
- W3159228535 cites W2075624130 @default.
- W3159228535 cites W2081422634 @default.
- W3159228535 cites W2084032366 @default.
- W3159228535 cites W2084802749 @default.
- W3159228535 cites W2091979095 @default.
- W3159228535 cites W2098115340 @default.
- W3159228535 cites W2102450255 @default.
- W3159228535 cites W2109577048 @default.
- W3159228535 cites W2116278708 @default.
- W3159228535 cites W2117442580 @default.
- W3159228535 cites W2120396047 @default.
- W3159228535 cites W2129095580 @default.
- W3159228535 cites W2131341690 @default.
- W3159228535 cites W2133298156 @default.
- W3159228535 cites W2134505828 @default.
- W3159228535 cites W2138207763 @default.
- W3159228535 cites W2140437023 @default.
- W3159228535 cites W2141261653 @default.
- W3159228535 cites W2141512206 @default.
- W3159228535 cites W2143519768 @default.
- W3159228535 cites W2147709159 @default.
- W3159228535 cites W2155753085 @default.
- W3159228535 cites W2160697532 @default.
- W3159228535 cites W2160761647 @default.
- W3159228535 cites W2163472330 @default.
- W3159228535 cites W2165869860 @default.
- W3159228535 cites W2174884009 @default.
- W3159228535 cites W2179438025 @default.
- W3159228535 cites W2236075974 @default.
- W3159228535 cites W2286847701 @default.
- W3159228535 cites W2309000102 @default.
- W3159228535 cites W2416906207 @default.
- W3159228535 cites W2466178221 @default.
- W3159228535 cites W2502874068 @default.
- W3159228535 cites W2509300015 @default.
- W3159228535 cites W2518075094 @default.
- W3159228535 cites W2518248674 @default.
- W3159228535 cites W2531734803 @default.
- W3159228535 cites W2558000280 @default.
- W3159228535 cites W2559191318 @default.
- W3159228535 cites W2573613735 @default.
- W3159228535 cites W2581904218 @default.
- W3159228535 cites W2624919703 @default.
- W3159228535 cites W2735120505 @default.
- W3159228535 cites W2753409843 @default.
- W3159228535 cites W2761439032 @default.
- W3159228535 cites W2767898033 @default.
- W3159228535 cites W2902919469 @default.
- W3159228535 cites W2906060794 @default.
- W3159228535 cites W2912990441 @default.
- W3159228535 cites W2914197015 @default.
- W3159228535 cites W2923103077 @default.
- W3159228535 cites W2924165648 @default.
- W3159228535 cites W2943124855 @default.
- W3159228535 cites W2945567049 @default.
- W3159228535 cites W2947494646 @default.