FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3159294513> ?p ?o ?g. }

• W3159294513 abstract "Abstract Biomolecular condensates organize biochemistry in time and space, yet little is known about how cells control either the position or scale of these assemblies. In cells, condensates often appear as dispersed, relatively small assemblies that do not grow (coarsen) into a single droplet despite their propensity to coalesce. Here we report that ribonucleoprotein condensates of the Q-rich protein Whi3 interact with the endoplasmic reticulum, prompting us to hypothesize that membrane association controls the position and size of condensates. Reconstitution of Whi3 condensates on supported lipid bilayers reveals that association with a diffusive lipid surface promotes condensation at both physiological ionic strength and protein concentration. Notably, these assemblies rapidly arrest, matching size distributions seen in cells. The timing of the arrest is influenced by the ordering of protein-protein and protein-RNA interactions and controlled by the slow diffusion of complexes induced by the membrane. This slowed diffusion limits both transfer of small protein-RNA complexes between condensates and their coalescence, thus driving coarsening to arrest. Our experiments reveal a tradeoff between locally-enhanced protein concentration at membranes, which favors condensation, and an accompanying reduction in diffusion, which restricts coarsening. Thus, membranes can maintain a population of small condensates in the absence of active mechanisms. Given that many condensates are bound to endomembranes, we predict that the biophysical properties of lipid bilayers are key for controlling condensate sizes throughout the cell. One sentence summary Assembly on a membrane surface positions and scales biomolecular condensates by controlling relative diffusion rates of proteins and nucleic acids." @default.
• W3159294513 created "2021-05-10" @default.
• W3159294513 creator A5007117633 @default.
• W3159294513 creator A5032449475 @default.
• W3159294513 creator A5048881160 @default.
• W3159294513 creator A5061952027 @default.
• W3159294513 creator A5069589438 @default.
• W3159294513 date "2021-04-24" @default.
• W3159294513 modified "2023-10-03" @default.
• W3159294513 title "Membrane surfaces regulate assembly of a ribonucleoprotein condensate" @default.
• W3159294513 cites W1153021260 @default.
• W3159294513 cites W1854281301 @default.
• W3159294513 cites W1912455695 @default.
• W3159294513 cites W1963175326 @default.
• W3159294513 cites W1986141440 @default.
• W3159294513 cites W1992699490 @default.
• W3159294513 cites W1998263911 @default.
• W3159294513 cites W2003029750 @default.
• W3159294513 cites W2009956378 @default.
• W3159294513 cites W2014336792 @default.
• W3159294513 cites W2014574381 @default.
• W3159294513 cites W2023261130 @default.
• W3159294513 cites W2048939436 @default.
• W3159294513 cites W2054352933 @default.
• W3159294513 cites W2059433969 @default.
• W3159294513 cites W2066631634 @default.
• W3159294513 cites W2074247664 @default.
• W3159294513 cites W2085978470 @default.
• W3159294513 cites W2102898524 @default.
• W3159294513 cites W2111796925 @default.
• W3159294513 cites W2164322080 @default.
• W3159294513 cites W2165364754 @default.
• W3159294513 cites W2172098255 @default.
• W3159294513 cites W2225896490 @default.
• W3159294513 cites W2335345046 @default.
• W3159294513 cites W2461977200 @default.
• W3159294513 cites W2473392275 @default.
• W3159294513 cites W2590568317 @default.
• W3159294513 cites W2594130445 @default.
• W3159294513 cites W2694038906 @default.
• W3159294513 cites W2722642526 @default.
• W3159294513 cites W2736318834 @default.
• W3159294513 cites W2756821926 @default.
• W3159294513 cites W2776559018 @default.
• W3159294513 cites W2784297130 @default.
• W3159294513 cites W2808919170 @default.
• W3159294513 cites W2838851331 @default.
• W3159294513 cites W2889188325 @default.
• W3159294513 cites W2901758876 @default.
• W3159294513 cites W2908663603 @default.
• W3159294513 cites W2921733160 @default.
• W3159294513 cites W2922246560 @default.
• W3159294513 cites W2931894123 @default.
• W3159294513 cites W2952808786 @default.
• W3159294513 cites W2971448217 @default.
• W3159294513 cites W2971541306 @default.
• W3159294513 cites W2973290848 @default.
• W3159294513 cites W2980184617 @default.
• W3159294513 cites W3004009934 @default.
• W3159294513 cites W3004533093 @default.
• W3159294513 cites W3021462784 @default.
• W3159294513 cites W3025030301 @default.
• W3159294513 cites W3032510072 @default.
• W3159294513 cites W3046158099 @default.
• W3159294513 cites W3088955889 @default.
• W3159294513 cites W3099299356 @default.
• W3159294513 cites W3120920966 @default.
• W3159294513 cites W3123697097 @default.
• W3159294513 cites W3125009241 @default.
• W3159294513 cites W3127828229 @default.
• W3159294513 cites W3133975122 @default.
• W3159294513 cites W3135396596 @default.
• W3159294513 cites W3153580301 @default.
• W3159294513 doi "https://doi.org/10.1101/2021.04.24.441251" @default.
• W3159294513 hasPublicationYear "2021" @default.
• W3159294513 type Work @default.
• W3159294513 sameAs 3159294513 @default.
• W3159294513 citedByCount "8" @default.
• W3159294513 countsByYear W31592945132021 @default.
• W3159294513 countsByYear W31592945132022 @default.
• W3159294513 crossrefType "posted-content" @default.
• W3159294513 hasAuthorship W3159294513A5007117633 @default.
• W3159294513 hasAuthorship W3159294513A5032449475 @default.
• W3159294513 hasAuthorship W3159294513A5048881160 @default.
• W3159294513 hasAuthorship W3159294513A5061952027 @default.
• W3159294513 hasAuthorship W3159294513A5069589438 @default.
• W3159294513 hasBestOaLocation W31592945131 @default.
• W3159294513 hasConcept C104317684 @default.
• W3159294513 hasConcept C121332964 @default.
• W3159294513 hasConcept C12554922 @default.
• W3159294513 hasConcept C144024400 @default.
• W3159294513 hasConcept C149923435 @default.
• W3159294513 hasConcept C185592680 @default.
• W3159294513 hasConcept C2908647359 @default.
• W3159294513 hasConcept C41625074 @default.
• W3159294513 hasConcept C55493867 @default.
• W3159294513 hasConcept C67705224 @default.
• W3159294513 hasConcept C69357855 @default.
• W3159294513 hasConcept C75934600 @default.
• W3159294513 hasConcept C86803240 @default.

• next