FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3159482771> ?p ?o ?g. }

• W3159482771 endingPage "122723" @default.
• W3159482771 startingPage "122723" @default.
• W3159482771 abstract "To optimize a screening method for macroprolactinemia and improve the accuracy of free prolactin (freePRL) detection. Overall efficiency, calculated as the product of the immunoglobulin G (IgG) precipitation rate and the freePRL recovery rate were employed to determine the concentration of the precipitant polyethylene glycol (PEG). Then, an optimized screening method for macroprolactinemia was established. The concentrations of freePRL, obtained by gel filtration chromatography (GFC), from 66 cases were used as the gold standard, and the sensitivity, specificity, accuracy and precision of the optimized and traditional methods for detecting macroprolactinemia were compared. (1) The IgG precipitation rate increased with increasing PEG6000 concentration, and the freePRL recovery rate decreased with increasing PEG6000 concentration; the overall efficiency first increased and then decreased. When the IgG concentrations in the mixture were 10 g/L, 25 g/L and 40 g/L, the concentrations of PEG6000 with the highest overall efficiency were 24%, 20% and 18%, respectively. (2) The effect of high and low IgG on the overall efficiency was 4.7% when using 20% PEG6000, which was lower than the effects when using 18% or 24% PEG6000 (9.2% and 13.2%). (3) In the optimized method established using 20% PEG6000, the macroprolactin (macroPRL) chromatographic peak disappeared, but the freePRL chromatographic peak was retained. The sensitivity of this macroprolactinemia screening method was 96.7%, and the specificity was 100%. (4) The freePRL concentrations obtained by the optimized method for samples from 30 macroprolactinemia cases and 36 true hyperprolactinemia cases were 15.8 (10.2-21.4) ng/mL and 60.2 (51.8-79.9) ng/mL; the concentrations were similar to those obtained using the GFC method (16.3 (11.9-27.2) ng/mL and 68.1 (49.5-92.9) ng/mL, respectively (p > 0.05)) and higher than those obtained using the traditional method (9.1 (6.1-17.6) ng/mL and 51.4 (43.7-71.9) ng/mL), respectively, p < 0.05)). (5) The relative deviation between the optimized and GFC methods was -7.0%, which was significantly lower than the relative deviation between the traditional and GFC methods (-21.4%, p < 0.01). (6) The in-batch coefficients of variation (CVs) for the dual-level quality control materials measured by the optimized method were 1.88% and 1.87%, and the within-laboratory CVs were 2.55% and 2.29%, which were slightly lower than the in-batch CVs (1.93% and 2.81%) and within-laboratory CVs (2.75% and 2.81%) measured by the traditional method. The established optimized method for screening macroprolactinemia using 20% PEG6000 as a precipitant can completely precipitate macroPRL components and effectively retain freePRL components. Compared with traditional methods, the optimized method is simpler, more accurate and more stable for the quantitative detection of freePRL." @default.
• W3159482771 created "2021-05-10" @default.
• W3159482771 creator A5002068866 @default.
• W3159482771 creator A5019790793 @default.
• W3159482771 creator A5033243558 @default.
• W3159482771 creator A5037247848 @default.
• W3159482771 creator A5041440075 @default.
• W3159482771 creator A5060905856 @default.
• W3159482771 creator A5060987863 @default.
• W3159482771 creator A5072800115 @default.
• W3159482771 date "2021-06-01" @default.
• W3159482771 modified "2023-09-27" @default.
• W3159482771 title "Optimization of a Screening Method for Macroprolactinemia" @default.
• W3159482771 cites W1978491858 @default.
• W3159482771 cites W1979694196 @default.
• W3159482771 cites W2003850616 @default.
• W3159482771 cites W2005971031 @default.
• W3159482771 cites W2019055295 @default.
• W3159482771 cites W2032275247 @default.
• W3159482771 cites W2032849314 @default.
• W3159482771 cites W2033556126 @default.
• W3159482771 cites W2071492646 @default.
• W3159482771 cites W2083780146 @default.
• W3159482771 cites W2087239362 @default.
• W3159482771 cites W2111154179 @default.
• W3159482771 cites W2116305496 @default.
• W3159482771 cites W2123886036 @default.
• W3159482771 cites W2156602436 @default.
• W3159482771 cites W2190043397 @default.
• W3159482771 cites W2406184269 @default.
• W3159482771 cites W2593674762 @default.
• W3159482771 cites W2809316841 @default.
• W3159482771 cites W2942942354 @default.
• W3159482771 cites W2953328078 @default.
• W3159482771 cites W2996605234 @default.
• W3159482771 cites W4211199751 @default.
• W3159482771 doi "https://doi.org/10.1016/j.jchromb.2021.122723" @default.
• W3159482771 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33989987" @default.
• W3159482771 hasPublicationYear "2021" @default.
• W3159482771 type Work @default.
• W3159482771 sameAs 3159482771 @default.
• W3159482771 citedByCount "3" @default.
• W3159482771 countsByYear W31594827712021 @default.
• W3159482771 countsByYear W31594827712023 @default.
• W3159482771 crossrefType "journal-article" @default.
• W3159482771 hasAuthorship W3159482771A5002068866 @default.
• W3159482771 hasAuthorship W3159482771A5019790793 @default.
• W3159482771 hasAuthorship W3159482771A5033243558 @default.
• W3159482771 hasAuthorship W3159482771A5037247848 @default.
• W3159482771 hasAuthorship W3159482771A5041440075 @default.
• W3159482771 hasAuthorship W3159482771A5060905856 @default.
• W3159482771 hasAuthorship W3159482771A5060987863 @default.
• W3159482771 hasAuthorship W3159482771A5072800115 @default.
• W3159482771 hasConcept C10138342 @default.
• W3159482771 hasConcept C162324750 @default.
• W3159482771 hasConcept C185592680 @default.
• W3159482771 hasConcept C2776964284 @default.
• W3159482771 hasConcept C43617362 @default.
• W3159482771 hasConcept C54400483 @default.
• W3159482771 hasConcept C55493867 @default.
• W3159482771 hasConceptScore W3159482771C10138342 @default.
• W3159482771 hasConceptScore W3159482771C162324750 @default.
• W3159482771 hasConceptScore W3159482771C185592680 @default.
• W3159482771 hasConceptScore W3159482771C2776964284 @default.
• W3159482771 hasConceptScore W3159482771C43617362 @default.
• W3159482771 hasConceptScore W3159482771C54400483 @default.
• W3159482771 hasConceptScore W3159482771C55493867 @default.
• W3159482771 hasFunder F4320338465 @default.
• W3159482771 hasLocation W31594827711 @default.
• W3159482771 hasOpenAccess W3159482771 @default.
• W3159482771 hasPrimaryLocation W31594827711 @default.
• W3159482771 hasRelatedWork W1966490926 @default.
• W3159482771 hasRelatedWork W2037651561 @default.
• W3159482771 hasRelatedWork W2119261566 @default.
• W3159482771 hasRelatedWork W2150895058 @default.
• W3159482771 hasRelatedWork W2313903280 @default.
• W3159482771 hasRelatedWork W2322734595 @default.
• W3159482771 hasRelatedWork W2356915299 @default.
• W3159482771 hasRelatedWork W2905499538 @default.
• W3159482771 hasRelatedWork W3141521293 @default.
• W3159482771 hasRelatedWork W4237696495 @default.
• W3159482771 hasVolume "1175" @default.
• W3159482771 isParatext "false" @default.
• W3159482771 isRetracted "false" @default.
• W3159482771 magId "3159482771" @default.
• W3159482771 workType "article" @default.