Matches in SemOpenAlex for { <https://semopenalex.org/work/W3159811832> ?p ?o ?g. }
- W3159811832 endingPage "2140" @default.
- W3159811832 startingPage "2140" @default.
- W3159811832 abstract "Intraoperative radiotherapy (IORT) displays an increasingly used treatment option for early breast cancer. It exhibits non-inferiority concerning the risk of recurrence compared to conventional external irradiation (EBRT) in suitable patients with early breast cancer. Since most relapses occur in direct proximity of the former tumor site, the reduction of the risk of local recurrence effected by radiotherapy might partially be due to an alteration of the irradiated tumor bed’s micromilieu. Our aim was to investigate if IORT affects the local micromilieu, especially immune cells with concomitant cytokine profile, and if it has an impact on growth conditions for breast cancer cells as well as mammary mesenchymal stromal cells (MSC), the latter considered as a model of the tumor bed stroma.42 breast cancer patients with breast-conserving surgery were included, of whom 21 received IORT (IORT group) and 21 underwent surgery without IORT (control group). Drainage wound fluid (WF) was collected from both groups 24 h after surgery for flow cytometric analysis of immune cell subset counts and potential apoptosis and for multiplex cytokine analyses (cytokine array and ELISA). It served further as a supplement in cultures of MDA-MB 231 breast cancer cells and mammary MSC for functional analyses, including proliferation, wound healing and migration. Furthermore, the cytokine profile within conditioned media from WF-treated MSC cultures was assessed. Flow cytometric analysis showed no group-related changes of cell count, activation state and apoptosis rates of myeloid, lymphoid leucocytes and regulatory T cells in the WF. Multiplex cytokine analysis of the WF revealed group-related differences in the expression levels of several cytokines, e.g., oncostatin-M, leptin and IL-1β. The application of WF in MDA-MB 231 cultures did not show a group-related difference in proliferation, wound healing and chemotactic migration. However, WF from IORT-treated patients significantly inhibited mammary MSC proliferation, wound healing and migration compared to WF from the control group. The conditioned media collected from WF-treated MSC-cultures also exhibited altered concentrations of VEGF, RANTES and GROα. IORT causes significant changes in the cytokine profile and MSC growth behavior. These changes in the tumor bed could potentially contribute to the beneficial oncological outcome entailed by this technique. The consideration whether this alteration also affects MSC interaction with other stroma components presents a promising gateway for future investigations." @default.
- W3159811832 created "2021-05-10" @default.
- W3159811832 creator A5025667256 @default.
- W3159811832 creator A5035403118 @default.
- W3159811832 creator A5052010172 @default.
- W3159811832 creator A5062821705 @default.
- W3159811832 creator A5073613423 @default.
- W3159811832 creator A5080800402 @default.
- W3159811832 creator A5088247085 @default.
- W3159811832 date "2021-04-29" @default.
- W3159811832 modified "2023-10-16" @default.
- W3159811832 title "Wound Fluid from Breast Cancer Patients Undergoing Intraoperative Radiotherapy Exhibits an Altered Cytokine Profile and Impairs Mesenchymal Stromal Cell Function" @default.
- W3159811832 cites W1949832432 @default.
- W3159811832 cites W1973993280 @default.
- W3159811832 cites W1983262355 @default.
- W3159811832 cites W1986182051 @default.
- W3159811832 cites W1991701652 @default.
- W3159811832 cites W2001356286 @default.
- W3159811832 cites W2004247659 @default.
- W3159811832 cites W2009197694 @default.
- W3159811832 cites W2021486359 @default.
- W3159811832 cites W2023057901 @default.
- W3159811832 cites W2027963618 @default.
- W3159811832 cites W2029520352 @default.
- W3159811832 cites W2032461554 @default.
- W3159811832 cites W2039112669 @default.
- W3159811832 cites W2044280478 @default.
- W3159811832 cites W2045929349 @default.
- W3159811832 cites W2046809995 @default.
- W3159811832 cites W2073821438 @default.
- W3159811832 cites W2081233470 @default.
- W3159811832 cites W2081843874 @default.
- W3159811832 cites W2086843919 @default.
- W3159811832 cites W2098949852 @default.
- W3159811832 cites W2100025800 @default.
- W3159811832 cites W2105054044 @default.
- W3159811832 cites W2109663505 @default.
- W3159811832 cites W2110603890 @default.
- W3159811832 cites W2117164669 @default.
- W3159811832 cites W2141815918 @default.
- W3159811832 cites W2147083075 @default.
- W3159811832 cites W2158615473 @default.
- W3159811832 cites W2167140474 @default.
- W3159811832 cites W2169670265 @default.
- W3159811832 cites W2169734442 @default.
- W3159811832 cites W2169863920 @default.
- W3159811832 cites W2171279313 @default.
- W3159811832 cites W2171717567 @default.
- W3159811832 cites W2172122796 @default.
- W3159811832 cites W2287449522 @default.
- W3159811832 cites W2511036853 @default.
- W3159811832 cites W2563743072 @default.
- W3159811832 cites W2563791715 @default.
- W3159811832 cites W2580035316 @default.
- W3159811832 cites W2595570239 @default.
- W3159811832 cites W2598370682 @default.
- W3159811832 cites W2618385741 @default.
- W3159811832 cites W2619237574 @default.
- W3159811832 cites W2743589051 @default.
- W3159811832 cites W2790676761 @default.
- W3159811832 cites W2795960234 @default.
- W3159811832 cites W2797407068 @default.
- W3159811832 cites W2801631929 @default.
- W3159811832 cites W2888320733 @default.
- W3159811832 cites W2907262288 @default.
- W3159811832 cites W2921434986 @default.
- W3159811832 cites W2946930934 @default.
- W3159811832 cites W2982163696 @default.
- W3159811832 cites W2995594096 @default.
- W3159811832 cites W3004538780 @default.
- W3159811832 cites W3004739760 @default.
- W3159811832 cites W3067502370 @default.
- W3159811832 cites W3090309351 @default.
- W3159811832 cites W4210427950 @default.
- W3159811832 doi "https://doi.org/10.3390/cancers13092140" @default.
- W3159811832 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8124792" @default.
- W3159811832 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33946741" @default.
- W3159811832 hasPublicationYear "2021" @default.
- W3159811832 type Work @default.
- W3159811832 sameAs 3159811832 @default.
- W3159811832 citedByCount "6" @default.
- W3159811832 countsByYear W31598118322021 @default.
- W3159811832 countsByYear W31598118322022 @default.
- W3159811832 countsByYear W31598118322023 @default.
- W3159811832 crossrefType "journal-article" @default.
- W3159811832 hasAuthorship W3159811832A5025667256 @default.
- W3159811832 hasAuthorship W3159811832A5035403118 @default.
- W3159811832 hasAuthorship W3159811832A5052010172 @default.
- W3159811832 hasAuthorship W3159811832A5062821705 @default.
- W3159811832 hasAuthorship W3159811832A5073613423 @default.
- W3159811832 hasAuthorship W3159811832A5080800402 @default.
- W3159811832 hasAuthorship W3159811832A5088247085 @default.
- W3159811832 hasBestOaLocation W31598118321 @default.
- W3159811832 hasConcept C121608353 @default.
- W3159811832 hasConcept C126322002 @default.
- W3159811832 hasConcept C142724271 @default.
- W3159811832 hasConcept C143998085 @default.
- W3159811832 hasConcept C16930146 @default.