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- W3160218405 abstract "Cell wall invertase (CWIN) activity and the expression of the corresponding gene were previously observed to be significantly elevated in a Cu-tolerant population of Elsholtzia haichowensis relative to a non-tolerant population under copper stress. To understand the differences in CWIN gene regulation between the two populations, their CWIN promoter β-glucuronidase (GUS) reporter vectors were constructed. GUS activity was measured in transgenic Arabidopsis in response to copper, sugar, and phytohormone treatments. Under the copper treatment, only the activity of the CWIN promoter from the Cu-tolerant population was slightly increased. Glucose and fructose significantly induced the activity of CWIN promoters from both populations. Among the phytohormone treatments, only salicylic acid induced significantly higher (p < 0.05) activity of the Cu-tolerant CWIN promoter relative to the non-tolerant promoters. Analysis of 5′-deletion constructs revealed that a 270-bp promoter fragment was required for SA induction of the promoter from the Cu-tolerant population. Comparison of this region in the two CWIN promoters revealed that it had 10 mutation sites and contained CAAT-box and W-box cis-elements in the Cu-tolerant promoter only. This work provides insights into the regulatory role of SA in CWIN gene expression and offers an explanation for differences in CWIN expression between E. haichowensis populations." @default.
- W3160218405 created "2021-05-24" @default.
- W3160218405 creator A5011833651 @default.
- W3160218405 creator A5022525914 @default.
- W3160218405 creator A5057508449 @default.
- W3160218405 creator A5088215369 @default.
- W3160218405 date "2021-05-18" @default.
- W3160218405 modified "2023-09-27" @default.
- W3160218405 title "Comparison and Characterization of a Cell Wall Invertase Promoter from Cu-Tolerant and Non-Tolerant Populations of Elsholtzia haichowensis" @default.
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- W3160218405 doi "https://doi.org/10.3390/ijms22105299" @default.
- W3160218405 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8157609" @default.
- W3160218405 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34069912" @default.
- W3160218405 hasPublicationYear "2021" @default.
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