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- W3160636537 abstract "ABSTRACT The Tup1-Cyc8 corepressor complex of Saccharomyces cerevisiae is recruited to promoters by DNA-binding proteins to repress transcription of genes, including the a -specific mating type genes. We report here a tup1(S649F) mutant that displays mating irregularities similar to a tup1 null and an α-predominant growth defect. RNA-Seq and ChIP-Seq were used to analyze gene expression and Tup1 occupancy changes in mutant vs. wild-type in both a and α cells. Increased Tup1(S649F) occupancy tended to occur upstream of upregulated genes, whereas locations with decreased occupancy usually did not show changes in gene expression, suggesting this mutant not only loses corepressor function but also behaves as a coactivator. Based upon studies demonstrating a dual role of Tup1 in both repression and activation, we postulate that the coactivator function of Tup1(S649F) results from diminished interaction with repressor proteins, including α2. We also found that large changes in mating type-specific gene expression between a and α or between mutant and wild-type were not easily explained by the range of Tup1 occupancy levels within their promoters, as predicted by the classic model of a -specific gene repression by Tup1. Most surprisingly, we observed Tup1 occupancy upstream of the a -specific gene MFA2 and the α-specific gene MF(ALPHA)1 in cells in which each gene was expressed rather than repressed. These results, combined with identification of additional mating related genes upregulated in the tup1(S649F) α strain, illustrate that the role of Tup1 in distinguishing mating types in yeast appears to be both more comprehensive and more nuanced than previously appreciated." @default.
- W3160636537 created "2021-05-24" @default.
- W3160636537 creator A5016244814 @default.
- W3160636537 creator A5016436397 @default.
- W3160636537 creator A5072166104 @default.
- W3160636537 date "2021-05-11" @default.
- W3160636537 modified "2023-09-23" @default.
- W3160636537 title "Genetic analysis argues for a coactivator function for the<i>Saccharomyces cerevisiae</i>Tup1 corepressor" @default.
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