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• W3160717774 abstract "Abstract Viral laboratory evolution has been used for different applications, such as modeling viral emergence, drug-resistance prediction, and therapeutic virus optimization. However, these studies have been mainly performed in cell monolayers, a highly simplified environment, raising concerns about their applicability and relevance. To address this, we compared the evolution of a model virus in monolayers, spheroids, and tissue explants. We performed this analysis in the context of cancer virotherapy by performing serial transfers of an oncolytic vesicular stomatitis virus (VSV-Δ51) in 4T1 mouse mammary tumor cells. We found that VSV-Δ51 gained fitness in each of these three culture systems, and that adaptation to the more complex environments (spheroids or explants) correlated with increased fitness in monolayers. Most evolved lines improved their ability to suppress β-interferon secretion compared to the VSV-Δ51 founder, suggesting that the selective pressure exerted by antiviral innate immunity was important in the three systems. However, system-specific patterns were also found. First, viruses evolved in monolayers remained more oncoselective that those evolved in spheroids, since the latter showed concomitant adaptation to non-tumoral mouse cells. Second, deep sequencing indicated that viral populations evolved in monolayers or explants tended to be more genetically diverse than those evolved in spheroids. Finally, we found highly variable outcomes among independent evolutionary lines propagated in explants. We conclude that experimental evolution in monolayers tends to be more reproducible than in spheroids or explants, and better preserves oncoselectivity. Our results also suggest that monolayers capture at least some relevant selective pressures present in more complex systems." @default.
• W3160717774 created "2021-05-24" @default.
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• W3160717774 date "2021-01-01" @default.
• W3160717774 modified "2023-09-23" @default.
• W3160717774 title "Experimental virus evolution in cancer cell monolayers, spheroids, and tissue explants" @default.
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• W3160717774 doi "https://doi.org/10.1093/ve/veab045" @default.
• W3160717774 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8134955" @default.
• W3160717774 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34040797" @default.
• W3160717774 hasPublicationYear "2021" @default.
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