FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3160959930> ?p ?o ?g. }

• W3160959930 abstract "Abstract The gram-positive bacterium Bacillus licheniformis exhibits obvious selective utilization on carbon sources. This process is mainly governed by the global regulator catabolite control protein A (CcpA), which can recognize and bind to multiple target genes widely distributed in metabolic pathways. Although the DNA-binding domain of CcpA has been predicted, the influence of key amino acids on target gene recognition and binding remains elusive. In this study, the impact of Lys31, Ile42 and Leu56 on in vitro protein-DNA interactions and in vivo carbon source selective utilization was investigated. The results showed that alanine substitution of Lys31 and Ile42, located within the 3 rd helices of the DNA-binding domain, significantly weakened the binding strength between CcpA and target genes. These mutations also lead to alleviated repression of xylose utilization in the presence of glucose. On the other hand, the Leu56Arg mutant in the 4 th helices exhibited enhanced binding affinity compared with that of the wild-type one. When this mutant was used to replace the native one in B. licheniformis cells, the selective utilization of glucose over xylose increased. The above research results are helpful for a deep understanding of how microorganisms can flexibly sense and adapt to changes in the external environment. Additionally, they can provide important theoretical basis for the rational design of biomass utilization and environmental adaptability of B. licheniformis cell factories. Importance Bacillus licheniformis is widely used in producing various valuable products, such as α enzymes, industrial chemicals and biocides. The carbon catabolite regulation process in the utilization of raw materials is crucial to maximizing the efficiency of this microbial cell factory. CcpA plays an important role in this process. This study represents a new paradigm to investigate the structure–function relationship in CcpA by fluorescence polarization experiments in vitro. The results also uncover key amino acids in the DNA-binding domain that affect the selective utilization of carbon sources. These results provide a theoretical basis for the rational design of industrial microorganisms." @default.
• W3160959930 created "2021-05-24" @default.
• W3160959930 creator A5001229547 @default.
• W3160959930 creator A5013378577 @default.
• W3160959930 creator A5020209614 @default.
• W3160959930 creator A5026578222 @default.
• W3160959930 creator A5027046605 @default.
• W3160959930 creator A5034246486 @default.
• W3160959930 creator A5046262151 @default.
• W3160959930 creator A5062429074 @default.
• W3160959930 creator A5082989901 @default.
• W3160959930 date "2021-05-18" @default.
• W3160959930 modified "2023-09-26" @default.
• W3160959930 title "Modulating the selective utilization of carbon sources by engineering the 3rd and 4th helices of the DNA-binding domain of catabolite control protein A (CcpA) in Bacillus licheniformis" @default.
• W3160959930 cites W1909716768 @default.
• W3160959930 cites W1964967532 @default.
• W3160959930 cites W1989519684 @default.
• W3160959930 cites W1997086452 @default.
• W3160959930 cites W2001901224 @default.
• W3160959930 cites W2022381548 @default.
• W3160959930 cites W2023816844 @default.
• W3160959930 cites W2032767027 @default.
• W3160959930 cites W2037722742 @default.
• W3160959930 cites W2049456105 @default.
• W3160959930 cites W2059849803 @default.
• W3160959930 cites W2060809301 @default.
• W3160959930 cites W2064414100 @default.
• W3160959930 cites W2066825888 @default.
• W3160959930 cites W2071486470 @default.
• W3160959930 cites W2072920810 @default.
• W3160959930 cites W2076458032 @default.
• W3160959930 cites W2082093843 @default.
• W3160959930 cites W2091756753 @default.
• W3160959930 cites W2102993309 @default.
• W3160959930 cites W2105115538 @default.
• W3160959930 cites W2107330312 @default.
• W3160959930 cites W2110919001 @default.
• W3160959930 cites W2117760833 @default.
• W3160959930 cites W2122041607 @default.
• W3160959930 cites W2127082390 @default.
• W3160959930 cites W2137625483 @default.
• W3160959930 cites W2143206518 @default.
• W3160959930 cites W2146060128 @default.
• W3160959930 cites W2169174113 @default.
• W3160959930 cites W2169888488 @default.
• W3160959930 cites W2178394372 @default.
• W3160959930 cites W2570754700 @default.
• W3160959930 cites W2582814439 @default.
• W3160959930 cites W2740403323 @default.
• W3160959930 cites W2782246223 @default.
• W3160959930 cites W2783755818 @default.
• W3160959930 cites W2791574520 @default.
• W3160959930 cites W2888643895 @default.
• W3160959930 cites W2901775403 @default.
• W3160959930 cites W2922004616 @default.
• W3160959930 cites W2926398704 @default.
• W3160959930 cites W2974385930 @default.
• W3160959930 cites W3005918381 @default.
• W3160959930 cites W3017788589 @default.
• W3160959930 cites W3021566521 @default.
• W3160959930 cites W3024036164 @default.
• W3160959930 cites W3026330441 @default.
• W3160959930 cites W3042256952 @default.
• W3160959930 cites W3043352460 @default.
• W3160959930 cites W4231953069 @default.
• W3160959930 doi "https://doi.org/10.1101/2021.05.17.444589" @default.
• W3160959930 hasPublicationYear "2021" @default.
• W3160959930 type Work @default.
• W3160959930 sameAs 3160959930 @default.
• W3160959930 citedByCount "0" @default.
• W3160959930 crossrefType "posted-content" @default.
• W3160959930 hasAuthorship W3160959930A5001229547 @default.
• W3160959930 hasAuthorship W3160959930A5013378577 @default.
• W3160959930 hasAuthorship W3160959930A5020209614 @default.
• W3160959930 hasAuthorship W3160959930A5026578222 @default.
• W3160959930 hasAuthorship W3160959930A5027046605 @default.
• W3160959930 hasAuthorship W3160959930A5034246486 @default.
• W3160959930 hasAuthorship W3160959930A5046262151 @default.
• W3160959930 hasAuthorship W3160959930A5062429074 @default.
• W3160959930 hasAuthorship W3160959930A5082989901 @default.
• W3160959930 hasBestOaLocation W31609599301 @default.
• W3160959930 hasConcept C100544194 @default.
• W3160959930 hasConcept C104317684 @default.
• W3160959930 hasConcept C143065580 @default.
• W3160959930 hasConcept C185592680 @default.
• W3160959930 hasConcept C2776527165 @default.
• W3160959930 hasConcept C2777272437 @default.
• W3160959930 hasConcept C2780294150 @default.
• W3160959930 hasConcept C2781225136 @default.
• W3160959930 hasConcept C523546767 @default.
• W3160959930 hasConcept C54355233 @default.
• W3160959930 hasConcept C552990157 @default.
• W3160959930 hasConcept C55493867 @default.
• W3160959930 hasConcept C83941689 @default.
• W3160959930 hasConcept C86803240 @default.
• W3160959930 hasConceptScore W3160959930C100544194 @default.
• W3160959930 hasConceptScore W3160959930C104317684 @default.
• W3160959930 hasConceptScore W3160959930C143065580 @default.
• W3160959930 hasConceptScore W3160959930C185592680 @default.
• W3160959930 hasConceptScore W3160959930C2776527165 @default.

• next