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- W3160993357 abstract "Abstract The hydrolysis of phosphatidylinositol 4,5− bisphosphate [PI(4,5)P 2 ] at the plasma membrane by receptor activated phospholipase C (PLC) activity is a conserved mechanism of signal transduction. Given the low abundance of PI(4,5)P 2 at the plasma membrane, its hydrolysis needs to be coupled to lipid resynthesis to ensure continued PLC activity during receptor activation. However, the mechanism by which PI(4,5)P 2 depletion during signalling is coupled to its resynthesis remains unknown. PI(4,5)P 2 synthesis is catalyzed by lipid kinase activity and the phosphorylation of phosphatidylinositol 4 phosphate (PI4P) by phosphatidylinositol 4 phosphate 5 kinase (PIP5K) is the final step in this process. In Drosophila photoreceptors, sensory transduction of photon absorption is transduced into PLC activity leading to an electrical response to light. During this process, PI(4,5)P 2 is resynthesized by a PIP5K activity but the mechanism by which the activity of this enzyme is coupled to PLC signalling is not known. In this study, we identify a unique protein isoform of dPIP5K, dPIP5K L that is both necessary and sufficient to mediate PI(4,5)P 2 synthesis during phototransduction. The activity of dPIP5K L in vitro is enhanced by depletion of PNUT, a non-redundant subunit of the septin family of GTP binding proteins and in vivo , depletion of pnut rescues the effect of dPIP5K L depletion on the light response and PI(4,5)P 2 resynthesis during PLC signalling. Lastly we find that depletion of Septin Interacting Protein 1 (Sip1), previously shown to bind PNUT, phenocopies the effect of dPIP5K L depletion in vivo . Thus, our work defines a septin 7 and Sip1 mediated mechanism through which PIP5K activity is coupled to ongoing PLC mediated PI(4,5)P 2 depletion." @default.
- W3160993357 created "2021-05-24" @default.
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- W3160993357 date "2021-05-17" @default.
- W3160993357 modified "2023-09-26" @default.
- W3160993357 title "Septin function tunes lipid kinase activity and phosphatidylinositol 4,5 bisphosphate turnover during G-protein coupled PLC signaling in vivo" @default.
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- W3160993357 doi "https://doi.org/10.1101/2021.05.14.444211" @default.
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