Matches in SemOpenAlex for { <https://semopenalex.org/work/W3161055929> ?p ?o ?g. }
- W3161055929 endingPage "109816" @default.
- W3161055929 startingPage "109816" @default.
- W3161055929 abstract "Allulose is an epimer of d-fructose with multiple nutritional functions, which can be biologically synthesized from d-fructose employing d-allulose 3-epimerase (DAE). In this study, NCBI database mining approach was applied to screen novel DAEs, and Novibacillus thermophilus DAE (NtDAE) exhibiting a high activity of 273.9 U/(g⋅wet cell weight) was selected for investigation. NtDAE showed optimum reaction temperature and pH at 70 °C and 7.0, respectively. In the presence of Co2+, its activity was improved by 100.1 %, and the half-life (t1/2) values reached up to 846.3, 539.2 and 47.8 min at 40, 50 and 60 °C, respectively. Higher kcat/Km values toward d-allulose indicated that the preferred physiological substrate of NtDAE was d-allulose. The conversion yield of 500 g/L d-fructose to d-allulose at 60 °C achieved 29.7 %, and further enhanced to 51.6 % with adding 1.1 M borate. This study provided a useful guidance of further engineering and application of NtDAE for realization of industrial production of d-allulose." @default.
- W3161055929 created "2021-05-24" @default.
- W3161055929 creator A5010741752 @default.
- W3161055929 creator A5026394330 @default.
- W3161055929 creator A5029724308 @default.
- W3161055929 creator A5048480543 @default.
- W3161055929 creator A5049584538 @default.
- W3161055929 creator A5058146813 @default.
- W3161055929 creator A5077209408 @default.
- W3161055929 creator A5082337686 @default.
- W3161055929 date "2021-08-01" @default.
- W3161055929 modified "2023-09-26" @default.
- W3161055929 title "Properties of d-allulose 3-epimerase mined from Novibacillus thermophilus and its application to synthesis of d-allulose" @default.
- W3161055929 cites W1963863483 @default.
- W3161055929 cites W1969996020 @default.
- W3161055929 cites W1970780806 @default.
- W3161055929 cites W1993348635 @default.
- W3161055929 cites W2002929422 @default.
- W3161055929 cites W2007828444 @default.
- W3161055929 cites W2009694869 @default.
- W3161055929 cites W2016899138 @default.
- W3161055929 cites W2043462765 @default.
- W3161055929 cites W2055448385 @default.
- W3161055929 cites W2058367205 @default.
- W3161055929 cites W2066519150 @default.
- W3161055929 cites W2150163188 @default.
- W3161055929 cites W2151389728 @default.
- W3161055929 cites W2160688731 @default.
- W3161055929 cites W2163679306 @default.
- W3161055929 cites W2170182584 @default.
- W3161055929 cites W2329160228 @default.
- W3161055929 cites W2411965021 @default.
- W3161055929 cites W2473020038 @default.
- W3161055929 cites W2529219625 @default.
- W3161055929 cites W2569056309 @default.
- W3161055929 cites W2573133088 @default.
- W3161055929 cites W2755945687 @default.
- W3161055929 cites W2767346686 @default.
- W3161055929 cites W2775810679 @default.
- W3161055929 cites W2788330318 @default.
- W3161055929 cites W2792315818 @default.
- W3161055929 cites W2803807846 @default.
- W3161055929 cites W2897282181 @default.
- W3161055929 cites W2908471907 @default.
- W3161055929 cites W2936313965 @default.
- W3161055929 cites W2951805046 @default.
- W3161055929 cites W2963186802 @default.
- W3161055929 cites W2995199302 @default.
- W3161055929 cites W3017118662 @default.
- W3161055929 cites W3044640728 @default.
- W3161055929 cites W3089105099 @default.
- W3161055929 cites W3112019984 @default.
- W3161055929 cites W744818514 @default.
- W3161055929 doi "https://doi.org/10.1016/j.enzmictec.2021.109816" @default.
- W3161055929 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34116747" @default.
- W3161055929 hasPublicationYear "2021" @default.
- W3161055929 type Work @default.
- W3161055929 sameAs 3161055929 @default.
- W3161055929 citedByCount "12" @default.
- W3161055929 countsByYear W31610559292021 @default.
- W3161055929 countsByYear W31610559292022 @default.
- W3161055929 countsByYear W31610559292023 @default.
- W3161055929 crossrefType "journal-article" @default.
- W3161055929 hasAuthorship W3161055929A5010741752 @default.
- W3161055929 hasAuthorship W3161055929A5026394330 @default.
- W3161055929 hasAuthorship W3161055929A5029724308 @default.
- W3161055929 hasAuthorship W3161055929A5048480543 @default.
- W3161055929 hasAuthorship W3161055929A5049584538 @default.
- W3161055929 hasAuthorship W3161055929A5058146813 @default.
- W3161055929 hasAuthorship W3161055929A5077209408 @default.
- W3161055929 hasAuthorship W3161055929A5082337686 @default.
- W3161055929 hasConcept C134121241 @default.
- W3161055929 hasConcept C185592680 @default.
- W3161055929 hasConcept C18903297 @default.
- W3161055929 hasConcept C191897082 @default.
- W3161055929 hasConcept C192562407 @default.
- W3161055929 hasConcept C203130289 @default.
- W3161055929 hasConcept C2776970464 @default.
- W3161055929 hasConcept C2777289219 @default.
- W3161055929 hasConcept C55493867 @default.
- W3161055929 hasConcept C71240020 @default.
- W3161055929 hasConcept C86803240 @default.
- W3161055929 hasConceptScore W3161055929C134121241 @default.
- W3161055929 hasConceptScore W3161055929C185592680 @default.
- W3161055929 hasConceptScore W3161055929C18903297 @default.
- W3161055929 hasConceptScore W3161055929C191897082 @default.
- W3161055929 hasConceptScore W3161055929C192562407 @default.
- W3161055929 hasConceptScore W3161055929C203130289 @default.
- W3161055929 hasConceptScore W3161055929C2776970464 @default.
- W3161055929 hasConceptScore W3161055929C2777289219 @default.
- W3161055929 hasConceptScore W3161055929C55493867 @default.
- W3161055929 hasConceptScore W3161055929C71240020 @default.
- W3161055929 hasConceptScore W3161055929C86803240 @default.
- W3161055929 hasLocation W31610559291 @default.
- W3161055929 hasOpenAccess W3161055929 @default.
- W3161055929 hasPrimaryLocation W31610559291 @default.
- W3161055929 hasRelatedWork W1986991025 @default.
- W3161055929 hasRelatedWork W2022995326 @default.