Matches in SemOpenAlex for { <https://semopenalex.org/work/W3161301883> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W3161301883 abstract "Background: Gain-of-Function (GOF) inflammasome mutations helped define the field of autoinflammation. Genotype/phenotype correlations both found within and between mutated genes that cause inflammasomopathies. We sought to identify features unique to NLRC4 GOF mutations and to develop a system for the unbiased assessment of Protein-Protein Interactions (PPi) made/lost by introduction of NLRC4 or NLRP3 inflammasome mutations in a myelomonocyte cell line.Methods: Part 1: Transduction of cell lines with WT or Mutant NLRC4 alone was preformed, followed by messenger RNA-sequencing and analysis. Part 2: Transduction of cell lines with WT or various of mutated forms of NLRP3 and NLRC4 was preformed to represent a diversity of clinical autoinflammatory phenotypes directly conjugated to the BirA biotinylase. Several experiments were conducted to assess biotinylation and confirm proper transduction. Interactome was established through the use of mass spectrometry. Results: Part 1: The most differentially expressed genes were evaluated. Unbiased GSEA analysis identified a strong IFN-induced gene signature among the most differentially expressed genes. Part 2: Inborn errors of immunity may be an initiating factor in the hyperactivation of the inflammasome.Public Health Relevance: The assessment of various mutations within the same gene or different genes thought to have similar functions may help to diagnose and investigate phenotypic outcomes of disease.Conclusion: NLRC4 may directly induce IFN production, potentially through a non-canonical pathway. Screening for interacting partners may identify the mechanism by which NLRP3 causes most IL-1β mediated disease and NLRC4 drives both Interleukin-1β (IL-1β) and Interleukin-18 (IL-18) production." @default.
- W3161301883 created "2021-05-24" @default.
- W3161301883 creator A5005811310 @default.
- W3161301883 date "2021-05-12" @default.
- W3161301883 modified "2023-09-24" @default.
- W3161301883 title "Unbiased Analysis of the Functions and Interactions of Human Inflammasome Mutations" @default.
- W3161301883 hasPublicationYear "2021" @default.
- W3161301883 type Work @default.
- W3161301883 sameAs 3161301883 @default.
- W3161301883 citedByCount "0" @default.
- W3161301883 crossrefType "journal-article" @default.
- W3161301883 hasAuthorship W3161301883A5005811310 @default.
- W3161301883 hasConcept C104317684 @default.
- W3161301883 hasConcept C127716648 @default.
- W3161301883 hasConcept C139964883 @default.
- W3161301883 hasConcept C143065580 @default.
- W3161301883 hasConcept C15152581 @default.
- W3161301883 hasConcept C170493617 @default.
- W3161301883 hasConcept C2777209026 @default.
- W3161301883 hasConcept C501734568 @default.
- W3161301883 hasConcept C54355233 @default.
- W3161301883 hasConcept C55493867 @default.
- W3161301883 hasConcept C56432331 @default.
- W3161301883 hasConcept C70721500 @default.
- W3161301883 hasConcept C86803240 @default.
- W3161301883 hasConcept C95444343 @default.
- W3161301883 hasConcept C9760119 @default.
- W3161301883 hasConceptScore W3161301883C104317684 @default.
- W3161301883 hasConceptScore W3161301883C127716648 @default.
- W3161301883 hasConceptScore W3161301883C139964883 @default.
- W3161301883 hasConceptScore W3161301883C143065580 @default.
- W3161301883 hasConceptScore W3161301883C15152581 @default.
- W3161301883 hasConceptScore W3161301883C170493617 @default.
- W3161301883 hasConceptScore W3161301883C2777209026 @default.
- W3161301883 hasConceptScore W3161301883C501734568 @default.
- W3161301883 hasConceptScore W3161301883C54355233 @default.
- W3161301883 hasConceptScore W3161301883C55493867 @default.
- W3161301883 hasConceptScore W3161301883C56432331 @default.
- W3161301883 hasConceptScore W3161301883C70721500 @default.
- W3161301883 hasConceptScore W3161301883C86803240 @default.
- W3161301883 hasConceptScore W3161301883C95444343 @default.
- W3161301883 hasConceptScore W3161301883C9760119 @default.
- W3161301883 hasLocation W31613018831 @default.
- W3161301883 hasOpenAccess W3161301883 @default.
- W3161301883 hasPrimaryLocation W31613018831 @default.
- W3161301883 hasRelatedWork W2043138173 @default.
- W3161301883 hasRelatedWork W2111723878 @default.
- W3161301883 hasRelatedWork W2137516695 @default.
- W3161301883 hasRelatedWork W2154363139 @default.
- W3161301883 hasRelatedWork W2314859925 @default.
- W3161301883 hasRelatedWork W2395559805 @default.
- W3161301883 hasRelatedWork W2765727939 @default.
- W3161301883 hasRelatedWork W2903604362 @default.
- W3161301883 hasRelatedWork W2910486407 @default.
- W3161301883 hasRelatedWork W2965609511 @default.
- W3161301883 hasRelatedWork W2985436419 @default.
- W3161301883 hasRelatedWork W2993401748 @default.
- W3161301883 hasRelatedWork W3033934322 @default.
- W3161301883 hasRelatedWork W3040679844 @default.
- W3161301883 hasRelatedWork W3097518634 @default.
- W3161301883 hasRelatedWork W3142149427 @default.
- W3161301883 hasRelatedWork W3165558181 @default.
- W3161301883 hasRelatedWork W3172648112 @default.
- W3161301883 hasRelatedWork W3179090996 @default.
- W3161301883 hasRelatedWork W3189629906 @default.
- W3161301883 isParatext "false" @default.
- W3161301883 isRetracted "false" @default.
- W3161301883 magId "3161301883" @default.
- W3161301883 workType "article" @default.