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• W3161416016 abstract "We describe a technique based on secondary ion mass spectrometry with nanoprojectiles (NP-SIMS) for determining the protein content of extracellular vesicles, EVs, via tagged antibodies. The technique uses individual gold nanoprojectiles (e.g., Au4004+ and Au28008+), separated in time and space, to bombard a surface. For each projectile impact (10–20 nm in diameter), the co-emitted molecules are mass analyzed and recorded as an individual mass spectrum. Examining these individual mass spectra for co-localized species allows for nanoscale mass spectrometry to be performed. The high lateral resolution of this technique is well suited for analyzing nano-objects. SIMS is generally limited to analyzing small molecules (below ∼1500 Da); therefore, we evaluated three molecules (eosin, erythrosine, and BHHTEGST) as prospective mass spectrometry tags. We tested these on a model surface comprising a mixture of all three tags conjugated to antibodies and found that NP-SIMS could detect all three tags from a single projectile impact. Applying the method, we tagged two surface proteins common in urinary EVs, CD63 and CD81, with anti-CD63–erythrosine and anti-CD81–BHHTEGST. We found that NP-SIMS could determine the relative abundance of the two proteins and required only a few hundred or thousand EVs in the analysis region to detect the presence of the tagged antibodies." @default.
• W3161416016 created "2021-05-24" @default.
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• W3161416016 date "2021-05-14" @default.
• W3161416016 modified "2023-10-03" @default.
• W3161416016 title "Nanoprojectile Secondary Ion Mass Spectrometry for Analysis of Extracellular Vesicles" @default.
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• W3161416016 doi "https://doi.org/10.1021/acs.analchem.1c00689" @default.
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• W3161416016 hasPublicationYear "2021" @default.
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