FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3161663710> ?p ?o ?g. }

• W3161663710 abstract "Abstract Binding sites of the chromatin regulator protein CTCF function as important landmarks in the human genome. The recently characterized CTCF-binding sites at LINE-1 repeats depend on another repeat-regulatory protein CGGBP1. These CGGBP1-dependent CTCF-binding sites serve as potential barrier elements for epigenetic marks such as H3K9me3. Such CTCF-binding sites are associated with asymmetric H3K9me3 levels as well as RNA levels in their flanks. The functions of these CGGBP1-dependent CTCF-binding sites remain un-known. By performing targeted studies on candidate CGGBP1-dependent CTCF-binding sites cloned in an SV40 promoter-enhancer episomal system we show that these regions act as inhibitors of ectopic transcription from the SV40 promoter. CGGBP1-dependent CTCF-binding sites that recapitulate their genomic function of loss of CTCF binding upon CGGBP1 depletion and H3K9me3 asymmetry in immediate flanks are also the ones that show the strongest inhibition of ectopic transcription. By performing a series of strand-specific reverse transcription PCRs we demonstrate that this ectopic transcription results in the synthesis of RNA from the SV40 promoter in a direction opposite to the downstream reporter gene in a strand-specific manner. The unleashing of the bidirectionality of the SV40 promoter activity and a breach of the transcription barrier seems to depend on depletion of CGGBP1 and loss of CTCF binding proximal to the SV40 promoter. RNA-sequencing reveals that CGGBP1-regulated CTCF-binding sites act as barriers to transcription at multiple locations genomewide. These findings suggest a role of CGGBP1-dependent binding sites in restricting ectopic transcription." @default.
• W3161663710 created "2021-05-24" @default.
• W3161663710 creator A5051970408 @default.
• W3161663710 creator A5069971140 @default.
• W3161663710 creator A5074682557 @default.
• W3161663710 creator A5090654807 @default.
• W3161663710 date "2020-11-10" @default.
• W3161663710 modified "2023-09-25" @default.
• W3161663710 title "CGGBP1-dependent CTCF-binding sites restrict ectopic transcription" @default.
• W3161663710 cites W1830661192 @default.
• W3161663710 cites W1838945435 @default.
• W3161663710 cites W1924378360 @default.
• W3161663710 cites W1967422137 @default.
• W3161663710 cites W1968884881 @default.
• W3161663710 cites W1970049297 @default.
• W3161663710 cites W1984827465 @default.
• W3161663710 cites W1985375619 @default.
• W3161663710 cites W1989373989 @default.
• W3161663710 cites W2019590714 @default.
• W3161663710 cites W2019874587 @default.
• W3161663710 cites W2021080331 @default.
• W3161663710 cites W2029669390 @default.
• W3161663710 cites W2046465127 @default.
• W3161663710 cites W2056627763 @default.
• W3161663710 cites W2070407195 @default.
• W3161663710 cites W2077908123 @default.
• W3161663710 cites W2078526277 @default.
• W3161663710 cites W2083026226 @default.
• W3161663710 cites W2089219831 @default.
• W3161663710 cites W2095560405 @default.
• W3161663710 cites W2095754453 @default.
• W3161663710 cites W2107032654 @default.
• W3161663710 cites W2111045993 @default.
• W3161663710 cites W2111243591 @default.
• W3161663710 cites W2144287097 @default.
• W3161663710 cites W2152968621 @default.
• W3161663710 cites W2158562128 @default.
• W3161663710 cites W2162972157 @default.
• W3161663710 cites W2166159761 @default.
• W3161663710 cites W217049847 @default.
• W3161663710 cites W2175811976 @default.
• W3161663710 cites W2192789229 @default.
• W3161663710 cites W2295297857 @default.
• W3161663710 cites W2341637133 @default.
• W3161663710 cites W2341987872 @default.
• W3161663710 cites W2463545287 @default.
• W3161663710 cites W2505829175 @default.
• W3161663710 cites W2518794193 @default.
• W3161663710 cites W2577253766 @default.
• W3161663710 cites W2774733631 @default.
• W3161663710 cites W2794410845 @default.
• W3161663710 cites W2810206188 @default.
• W3161663710 cites W2974907528 @default.
• W3161663710 cites W2975633123 @default.
• W3161663710 cites W2979771713 @default.
• W3161663710 cites W3007172120 @default.
• W3161663710 cites W3019559753 @default.
• W3161663710 cites W3045496042 @default.
• W3161663710 cites W4240375588 @default.
• W3161663710 cites W4246023066 @default.
• W3161663710 doi "https://doi.org/10.1101/2020.11.09.375782" @default.
• W3161663710 hasPublicationYear "2020" @default.
• W3161663710 type Work @default.
• W3161663710 sameAs 3161663710 @default.
• W3161663710 citedByCount "0" @default.
• W3161663710 crossrefType "posted-content" @default.
• W3161663710 hasAuthorship W3161663710A5051970408 @default.
• W3161663710 hasAuthorship W3161663710A5069971140 @default.
• W3161663710 hasAuthorship W3161663710A5074682557 @default.
• W3161663710 hasAuthorship W3161663710A5090654807 @default.
• W3161663710 hasBestOaLocation W31616637101 @default.
• W3161663710 hasConcept C101762097 @default.
• W3161663710 hasConcept C104317684 @default.
• W3161663710 hasConcept C107824862 @default.
• W3161663710 hasConcept C111936080 @default.
• W3161663710 hasConcept C119599485 @default.
• W3161663710 hasConcept C127413603 @default.
• W3161663710 hasConcept C138885662 @default.
• W3161663710 hasConcept C150194340 @default.
• W3161663710 hasConcept C153911025 @default.
• W3161663710 hasConcept C179926584 @default.
• W3161663710 hasConcept C212702 @default.
• W3161663710 hasConcept C2778760011 @default.
• W3161663710 hasConcept C41091548 @default.
• W3161663710 hasConcept C41895202 @default.
• W3161663710 hasConcept C54355233 @default.
• W3161663710 hasConcept C83640560 @default.
• W3161663710 hasConcept C86339819 @default.
• W3161663710 hasConcept C86803240 @default.
• W3161663710 hasConcept C95444343 @default.
• W3161663710 hasConcept C97037327 @default.
• W3161663710 hasConceptScore W3161663710C101762097 @default.
• W3161663710 hasConceptScore W3161663710C104317684 @default.
• W3161663710 hasConceptScore W3161663710C107824862 @default.
• W3161663710 hasConceptScore W3161663710C111936080 @default.
• W3161663710 hasConceptScore W3161663710C119599485 @default.
• W3161663710 hasConceptScore W3161663710C127413603 @default.
• W3161663710 hasConceptScore W3161663710C138885662 @default.
• W3161663710 hasConceptScore W3161663710C150194340 @default.
• W3161663710 hasConceptScore W3161663710C153911025 @default.

• next