Matches in SemOpenAlex for { <https://semopenalex.org/work/W3161998889> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W3161998889 endingPage "147" @default.
- W3161998889 startingPage "147" @default.
- W3161998889 abstract "Background: Hepatocellular carcinoma (HCC) is the third cause of cancer-related death worldwide. Curative options for HCC are only available for patients with tumor of an early stage, thus a precise method to detect HCC in its early stage is needed. MicroRNAs (miRNAs) are non-coding small (~25nt) RNAs that play important roles in gene regulatory networks by repressing the activity of specific target mRNAs. Aberrant expression of several miRNAs was found to be involved in human hepatocarcinogenesis. In this study we compared the expression levels of miRNAs between surgically resected HCC tissues and surrounding non-tumor tissues of 20 patients with HCC of early stages. Aims: The aim of this study is to identify miRNAs that can be used as biomarkers for an early stage HCC. Methods: miRNAs were isolated from surgically resected HCC tissues and surrounding non-tumor tissues of 20 HCC patients and used to synthesize cDNAs. We designed primers for 50 kinds of miRNAs, selection of which was based on published microarray studies. We performed SYBR Green quantitative RT-PCR (qRT-PCR) assays to compare expression levels of miRNAs between HCC and surrounding non-HCC tissues. Results: Six miRNAs were found highly up-regulated in early stage HCC. MiR-17-5p, 24, 25, 107, 221, and 222 were significantly up-regulated in 70%, 65%, 75%, 75%, 90%, and 85% of HCC samples, respectively. All of the HCC samples, at least one of miR-221 and miR-25 was up-regulated, as compared with adjacent non-tumor tissues. For mechanism study, we transfected the miRNAs expressing plasmids in HCC cell lines. MiR-221 enhanced cell proliferation in Huh7 cells. And miR- 221, 21-5p, 25, 222, and 17-5p are increased cell migration in Hep3B cells. Conclusions: We identified miRNAs which are significantly up-regulated in early stage HCC samples. All patients had an elevated expression level of either miR-221 or miR-25. We suggest that the combination of the two microRNAs might be an effective biomarker for detecting early HCC." @default.
- W3161998889 created "2021-05-24" @default.
- W3161998889 creator A5028586962 @default.
- W3161998889 creator A5037540651 @default.
- W3161998889 creator A5049575773 @default.
- W3161998889 creator A5054417741 @default.
- W3161998889 creator A5067263547 @default.
- W3161998889 creator A5069076427 @default.
- W3161998889 date "2013-01-01" @default.
- W3161998889 modified "2023-09-23" @default.
- W3161998889 title "Basic, Research : Expression Levels of Cancer-related microRNAs in Human Hepatocellular Carcinoma (HCC)" @default.
- W3161998889 hasPublicationYear "2013" @default.
- W3161998889 type Work @default.
- W3161998889 sameAs 3161998889 @default.
- W3161998889 citedByCount "0" @default.
- W3161998889 crossrefType "journal-article" @default.
- W3161998889 hasAuthorship W3161998889A5028586962 @default.
- W3161998889 hasAuthorship W3161998889A5037540651 @default.
- W3161998889 hasAuthorship W3161998889A5049575773 @default.
- W3161998889 hasAuthorship W3161998889A5054417741 @default.
- W3161998889 hasAuthorship W3161998889A5067263547 @default.
- W3161998889 hasAuthorship W3161998889A5069076427 @default.
- W3161998889 hasConcept C104317684 @default.
- W3161998889 hasConcept C121608353 @default.
- W3161998889 hasConcept C143998085 @default.
- W3161998889 hasConcept C145059251 @default.
- W3161998889 hasConcept C146357865 @default.
- W3161998889 hasConcept C150194340 @default.
- W3161998889 hasConcept C151730666 @default.
- W3161998889 hasConcept C186836561 @default.
- W3161998889 hasConcept C2778019345 @default.
- W3161998889 hasConcept C502942594 @default.
- W3161998889 hasConcept C54355233 @default.
- W3161998889 hasConcept C71924100 @default.
- W3161998889 hasConcept C86803240 @default.
- W3161998889 hasConceptScore W3161998889C104317684 @default.
- W3161998889 hasConceptScore W3161998889C121608353 @default.
- W3161998889 hasConceptScore W3161998889C143998085 @default.
- W3161998889 hasConceptScore W3161998889C145059251 @default.
- W3161998889 hasConceptScore W3161998889C146357865 @default.
- W3161998889 hasConceptScore W3161998889C150194340 @default.
- W3161998889 hasConceptScore W3161998889C151730666 @default.
- W3161998889 hasConceptScore W3161998889C186836561 @default.
- W3161998889 hasConceptScore W3161998889C2778019345 @default.
- W3161998889 hasConceptScore W3161998889C502942594 @default.
- W3161998889 hasConceptScore W3161998889C54355233 @default.
- W3161998889 hasConceptScore W3161998889C71924100 @default.
- W3161998889 hasConceptScore W3161998889C86803240 @default.
- W3161998889 hasIssue "1" @default.
- W3161998889 hasLocation W31619988891 @default.
- W3161998889 hasOpenAccess W3161998889 @default.
- W3161998889 hasPrimaryLocation W31619988891 @default.
- W3161998889 hasRelatedWork W1994788963 @default.
- W3161998889 hasRelatedWork W2096160281 @default.
- W3161998889 hasRelatedWork W2380040477 @default.
- W3161998889 hasRelatedWork W2740885133 @default.
- W3161998889 hasRelatedWork W2808487976 @default.
- W3161998889 hasRelatedWork W2888090881 @default.
- W3161998889 hasRelatedWork W2898979195 @default.
- W3161998889 hasRelatedWork W2903108737 @default.
- W3161998889 hasRelatedWork W2956656429 @default.
- W3161998889 hasRelatedWork W2971448879 @default.
- W3161998889 hasRelatedWork W2998367075 @default.
- W3161998889 hasRelatedWork W3006002721 @default.
- W3161998889 hasRelatedWork W3006287503 @default.
- W3161998889 hasRelatedWork W3009525626 @default.
- W3161998889 hasRelatedWork W3019607268 @default.
- W3161998889 hasRelatedWork W3026955411 @default.
- W3161998889 hasRelatedWork W3032710903 @default.
- W3161998889 hasRelatedWork W3145330856 @default.
- W3161998889 hasRelatedWork W3191341178 @default.
- W3161998889 hasRelatedWork W3193360164 @default.
- W3161998889 hasVolume "2013" @default.
- W3161998889 isParatext "false" @default.
- W3161998889 isRetracted "false" @default.
- W3161998889 magId "3161998889" @default.
- W3161998889 workType "article" @default.