FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3162132271> ?p ?o ?g. }

• W3162132271 abstract "ABSTRACT Background Coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 has now spread worldwide to infect over 110 million people, with approximately 2.5 million reported deaths. A safe and effective vaccine remains urgently needed. Method We constructed three variants of the recombinant receptor-binding domain (RBD) of the SARS-CoV-2 spike (S) protein (residues 331-549) in yeast as follows: (1) a “wild type” RBD (RBD219-WT), (2) a deglycosylated form (RBD219-N1) by deleting the first N-glycosylation site, and (3) a combined deglycosylated and cysteine-mutagenized form (C538A-mutated variant (RBD219-N1C1)). We compared the expression yields, biophysical characteristics, and functionality of the proteins produced from these constructs. Results and conclusions These three recombinant RBDs showed similar secondary and tertiary structure thermal stability and had the same affinity to their receptor, angiotensin-converting enzyme 2 (ACE-2), suggesting that the selected deletion or mutations did not cause any significant structural changes or alteration of function. However, RBD219-N1C1 had a higher fermentation yield, was easier to purify, was not hyperglycosylated, and had a lower tendency to form oligomers, and thus was selected for further vaccine development and evaluation. General significance By genetic modification, we were able to design a better-controlled and more stable vaccine candidate, which is an essential and important criterion for any process and manufacturing of biologics or drugs for human use." @default.
• W3162132271 created "2021-05-24" @default.
• W3162132271 creator A5009039459 @default.
• W3162132271 creator A5020338013 @default.
• W3162132271 creator A5020411177 @default.
• W3162132271 creator A5022322106 @default.
• W3162132271 creator A5034049607 @default.
• W3162132271 creator A5050311944 @default.
• W3162132271 creator A5056918526 @default.
• W3162132271 creator A5057678846 @default.
• W3162132271 creator A5068617182 @default.
• W3162132271 creator A5072496895 @default.
• W3162132271 creator A5074660106 @default.
• W3162132271 creator A5079281552 @default.
• W3162132271 creator A5082101414 @default.
• W3162132271 creator A5082820231 @default.
• W3162132271 creator A5085249714 @default.
• W3162132271 creator A5085813371 @default.
• W3162132271 creator A5087704375 @default.
• W3162132271 creator A5089923491 @default.
• W3162132271 date "2020-11-09" @default.
• W3162132271 modified "2023-10-16" @default.
• W3162132271 title "Genetic Modification to Design a Stable Yeast-expressed Recombinant SARS-CoV-2 Receptor Binding Domain as a COVID-19 Vaccine Candidate" @default.
• W3162132271 cites W1992722956 @default.
• W3162132271 cites W1994812138 @default.
• W3162132271 cites W2029314929 @default.
• W3162132271 cites W2029763364 @default.
• W3162132271 cites W2042169463 @default.
• W3162132271 cites W2046071548 @default.
• W3162132271 cites W2054609914 @default.
• W3162132271 cites W2081837522 @default.
• W3162132271 cites W2081861008 @default.
• W3162132271 cites W2092639010 @default.
• W3162132271 cites W2095633747 @default.
• W3162132271 cites W2144514674 @default.
• W3162132271 cites W2146476111 @default.
• W3162132271 cites W2154026160 @default.
• W3162132271 cites W2162791356 @default.
• W3162132271 cites W2325881814 @default.
• W3162132271 cites W2545435791 @default.
• W3162132271 cites W2609522093 @default.
• W3162132271 cites W2790068585 @default.
• W3162132271 cites W3004280078 @default.
• W3162132271 cites W3005625914 @default.
• W3162132271 cites W3007643904 @default.
• W3162132271 cites W3008443627 @default.
• W3162132271 cites W3009912996 @default.
• W3162132271 cites W3010780802 @default.
• W3162132271 cites W3015909209 @default.
• W3162132271 cites W3020577244 @default.
• W3162132271 cites W3023376619 @default.
• W3162132271 cites W3025744748 @default.
• W3162132271 cites W3037923073 @default.
• W3162132271 cites W3044660350 @default.
• W3162132271 cites W3045578670 @default.
• W3162132271 cites W3081892744 @default.
• W3162132271 cites W3087423707 @default.
• W3162132271 cites W3092403777 @default.
• W3162132271 cites W3092921648 @default.
• W3162132271 cites W3096064145 @default.
• W3162132271 cites W3119517955 @default.
• W3162132271 cites W3123423072 @default.
• W3162132271 doi "https://doi.org/10.1101/2020.11.09.373449" @default.
• W3162132271 hasPublicationYear "2020" @default.
• W3162132271 type Work @default.
• W3162132271 sameAs 3162132271 @default.
• W3162132271 citedByCount "6" @default.
• W3162132271 countsByYear W31621322712020 @default.
• W3162132271 countsByYear W31621322712021 @default.
• W3162132271 crossrefType "posted-content" @default.
• W3162132271 hasAuthorship W3162132271A5009039459 @default.
• W3162132271 hasAuthorship W3162132271A5020338013 @default.
• W3162132271 hasAuthorship W3162132271A5020411177 @default.
• W3162132271 hasAuthorship W3162132271A5022322106 @default.
• W3162132271 hasAuthorship W3162132271A5034049607 @default.
• W3162132271 hasAuthorship W3162132271A5050311944 @default.
• W3162132271 hasAuthorship W3162132271A5056918526 @default.
• W3162132271 hasAuthorship W3162132271A5057678846 @default.
• W3162132271 hasAuthorship W3162132271A5068617182 @default.
• W3162132271 hasAuthorship W3162132271A5072496895 @default.
• W3162132271 hasAuthorship W3162132271A5074660106 @default.
• W3162132271 hasAuthorship W3162132271A5079281552 @default.
• W3162132271 hasAuthorship W3162132271A5082101414 @default.
• W3162132271 hasAuthorship W3162132271A5082820231 @default.
• W3162132271 hasAuthorship W3162132271A5085249714 @default.
• W3162132271 hasAuthorship W3162132271A5085813371 @default.
• W3162132271 hasAuthorship W3162132271A5087704375 @default.
• W3162132271 hasAuthorship W3162132271A5089923491 @default.
• W3162132271 hasBestOaLocation W31621322711 @default.
• W3162132271 hasConcept C104317684 @default.
• W3162132271 hasConcept C142724271 @default.
• W3162132271 hasConcept C143065580 @default.
• W3162132271 hasConcept C159047783 @default.
• W3162132271 hasConcept C170493617 @default.
• W3162132271 hasConcept C185592680 @default.
• W3162132271 hasConcept C2777313579 @default.
• W3162132271 hasConcept C2779134260 @default.
• W3162132271 hasConcept C2779222958 @default.
• W3162132271 hasConcept C3008058167 @default.
• W3162132271 hasConcept C40767141 @default.

• next