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- W3162132619 abstract "L-Gulose is a rare aldohexose to serve as a building block for anticancer drug bleomycin and nucleoside-based antivirals. However, preparative inaccessibility and high cost have hindered its pharmaceutical application. Despite a regio- and stereo-selective enzymatic synthesis of l-gulose from d-sorbitol using a variant of NAD+-dependent mannitol-1-dehydrogenase from Apium graveolens (mMDH) was explored, low efficiency and productivity caused by NADH accumulation or insufficient amount of NAD+ limited the practical utility of this process. In this study, a stable and efficient NADH oxidase from Bacillus cereus (bcNOX) was found to be more compatible with mMDH to recycle NAD+ in E. coli cells for l-gulose biosynthesis. After a systematic optimization of the whole-cell system, efficient biosynthesis of l-gulose was achieved. Starting with 70 g/L of readily available and cheap d-sorbitol resulted in a volumetric productivity of 5.5 g/L/d. This whole-cell approach enables practical, efficient and environmentally friendly biosynthesis of l-gulose and exhibits the potential of becoming a biocatalytic strategy for various enzymatic oxidative transformations." @default.
- W3162132619 created "2021-05-24" @default.
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- W3162132619 date "2021-08-01" @default.
- W3162132619 modified "2023-09-25" @default.
- W3162132619 title "Efficient whole-cell biosynthesis of l-gulose by coupling mannitol-1-dehydrogenase with NADH oxidase" @default.
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- W3162132619 doi "https://doi.org/10.1016/j.enzmictec.2021.109815" @default.
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