Matches in SemOpenAlex for { <https://semopenalex.org/work/W3162328500> ?p ?o ?g. }
- W3162328500 endingPage "e1009544" @default.
- W3162328500 startingPage "e1009544" @default.
- W3162328500 abstract "Here we describe the development and characterization of the photo-N-degron, a peptide tag that can be used in optogenetic studies of protein function in vivo . The photo-N-degron can be expressed as a genetic fusion to the amino termini of other proteins, where it undergoes a blue light-dependent conformational change that exposes a signal for the class of ubiquitin ligases, the N-recognins, which mediate the N-end rule mechanism of proteasomal degradation. We demonstrate that the photo-N-degron can be used to direct light-mediated degradation of proteins in Saccharomyces cerevisiae and Drosophila melanogaster with fine temporal control. In addition, we compare the effectiveness of the photo-N-degron with that of two other light-dependent degrons that have been developed in their abilities to mediate the loss of function of Cactus, a component of the dorsal-ventral patterning system in the Drosophila embryo. We find that like the photo-N-degron, the blue light-inducible degradation (B-LID) domain, a light-activated degron that must be placed at the carboxy terminus of targeted proteins, is also effective in eliciting light-dependent loss of Cactus function, as determined by embryonic dorsal-ventral patterning phenotypes. In contrast, another previously described photosensitive degron (psd), which also must be located at the carboxy terminus of associated proteins, has little effect on Cactus-dependent phenotypes in response to illumination of developing embryos. These and other observations indicate that care must be taken in the selection and application of light-dependent and other inducible degrons for use in studies of protein function in vivo , but importantly demonstrate that N- and C-terminal fusions to the photo-N-degron and the B-LID domain, respectively, support light-dependent degradation in vivo ." @default.
- W3162328500 created "2021-05-24" @default.
- W3162328500 creator A5005248189 @default.
- W3162328500 creator A5006386713 @default.
- W3162328500 creator A5007637965 @default.
- W3162328500 creator A5043215626 @default.
- W3162328500 creator A5061293610 @default.
- W3162328500 creator A5069668374 @default.
- W3162328500 creator A5070254341 @default.
- W3162328500 date "2021-05-17" @default.
- W3162328500 modified "2023-10-13" @default.
- W3162328500 title "Light-dependent N-end rule-mediated disruption of protein function in Saccharomyces cerevisiae and Drosophila melanogaster" @default.
- W3162328500 cites W1483766000 @default.
- W3162328500 cites W1495373679 @default.
- W3162328500 cites W1585503692 @default.
- W3162328500 cites W1611419331 @default.
- W3162328500 cites W1826524642 @default.
- W3162328500 cites W1919687995 @default.
- W3162328500 cites W1931438735 @default.
- W3162328500 cites W1964819733 @default.
- W3162328500 cites W1965813899 @default.
- W3162328500 cites W1968074226 @default.
- W3162328500 cites W1974338734 @default.
- W3162328500 cites W1975437758 @default.
- W3162328500 cites W1976656176 @default.
- W3162328500 cites W1980490260 @default.
- W3162328500 cites W1980552600 @default.
- W3162328500 cites W1983816460 @default.
- W3162328500 cites W1985542063 @default.
- W3162328500 cites W1995893329 @default.
- W3162328500 cites W1997124556 @default.
- W3162328500 cites W1998820908 @default.
- W3162328500 cites W2000346699 @default.
- W3162328500 cites W2001831734 @default.
- W3162328500 cites W2002242109 @default.
- W3162328500 cites W2003001669 @default.
- W3162328500 cites W2003885556 @default.
- W3162328500 cites W2007457485 @default.
- W3162328500 cites W2009837683 @default.
- W3162328500 cites W2010056426 @default.
- W3162328500 cites W2012430176 @default.
- W3162328500 cites W2015127906 @default.
- W3162328500 cites W2020652110 @default.
- W3162328500 cites W2032387485 @default.
- W3162328500 cites W2035417449 @default.
- W3162328500 cites W2035424802 @default.
- W3162328500 cites W2037594700 @default.
- W3162328500 cites W2038591876 @default.
- W3162328500 cites W2040078255 @default.
- W3162328500 cites W2040444669 @default.
- W3162328500 cites W2048139403 @default.
- W3162328500 cites W2048599311 @default.
- W3162328500 cites W2050140768 @default.
- W3162328500 cites W2052538982 @default.
- W3162328500 cites W2054221638 @default.
- W3162328500 cites W2057120350 @default.
- W3162328500 cites W2057381460 @default.
- W3162328500 cites W2059843465 @default.
- W3162328500 cites W2063150941 @default.
- W3162328500 cites W2063210874 @default.
- W3162328500 cites W2071056869 @default.
- W3162328500 cites W2071238751 @default.
- W3162328500 cites W2071847586 @default.
- W3162328500 cites W2076854746 @default.
- W3162328500 cites W2078434863 @default.
- W3162328500 cites W2079139285 @default.
- W3162328500 cites W2079428226 @default.
- W3162328500 cites W2082411057 @default.
- W3162328500 cites W2082507962 @default.
- W3162328500 cites W2082523159 @default.
- W3162328500 cites W2082702196 @default.
- W3162328500 cites W2083758347 @default.
- W3162328500 cites W2086837225 @default.
- W3162328500 cites W2088145350 @default.
- W3162328500 cites W2093489683 @default.
- W3162328500 cites W2097028894 @default.
- W3162328500 cites W2097323162 @default.
- W3162328500 cites W2097350262 @default.
- W3162328500 cites W2107006351 @default.
- W3162328500 cites W2112938540 @default.
- W3162328500 cites W2112940322 @default.
- W3162328500 cites W2121260094 @default.
- W3162328500 cites W2122564020 @default.
- W3162328500 cites W2124833370 @default.
- W3162328500 cites W2126020222 @default.
- W3162328500 cites W2126895934 @default.
- W3162328500 cites W2129358952 @default.
- W3162328500 cites W2130002068 @default.
- W3162328500 cites W2134962337 @default.
- W3162328500 cites W2137117135 @default.
- W3162328500 cites W2140754284 @default.
- W3162328500 cites W2150972545 @default.
- W3162328500 cites W2152956404 @default.
- W3162328500 cites W2154395280 @default.
- W3162328500 cites W2155615473 @default.
- W3162328500 cites W2155775409 @default.
- W3162328500 cites W2157244412 @default.
- W3162328500 cites W2159137674 @default.