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• W3162502186 abstract "The dissection of the veins is the trickiest step of Uterine transplantation (UTx). Performing the anastomosis of a single uterine vein could bring a therapeutic benefit and simplification of surgery and serve for managing unilateral venous thromboses. The objectives of this project were to evaluate the expression of early markers of ischemia-reperfusion and to compare findings following one or two vein anastomoses. Orthotopic uterine auto-transplantations were performed on an ovine model with anastomosis of either two (group 1) or one utero-ovarian veins (group 2). Blood gases, histology and ischemia- reperfusion markers transcripts (PTGS2, IL6, IL8, SOD2, C3, BAX/BCL2 and TLR4) were analyzed as well as PTGS2 protein expression using Western Blot and fluorescence immunolocalization on endometrial biopsies after 3h of reperfusion. Ten ewes were included in the experimentation, 4 were in group1, 3 in group 2, the others being sham operated controls. No significant differences were observed between the two phenotypes. Based on these results, the anastomosis of one single uterine vein appears to be an approach consistent with short-term graft survival. Further experiments will be needed to confirm the reliability of this approach, especially the long-term follow-up of the uterine graft including its ability to support gestation to term." @default.
• W3162502186 created "2021-05-24" @default.
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• W3162502186 date "2021-05-18" @default.
• W3162502186 modified "2023-10-18" @default.
• W3162502186 title "Analysis of blood parameters and molecular endometrial markers during early reperfusion in two ovine models of uterus transplantation" @default.
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• W3162502186 doi "https://doi.org/10.1371/journal.pone.0251474" @default.
• W3162502186 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8130915" @default.
• W3162502186 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34003831" @default.
• W3162502186 hasPublicationYear "2021" @default.
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