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- W3162690981 abstract "Endogenous RNA interference (RNAi) pathways regulate a wide range of cellular processes in diverse eukaryotes, yet in the ciliated eukaryote, Tetrahymena thermophila, the cellular purpose of RNAi pathways that generate ∼23-24 nucleotide (nt) small (s)RNAs has remained unknown. Here, we investigated the phenotypic and gene expression impacts on vegetatively growing cells when genes involved in ∼23-24 nt sRNA biogenesis are disrupted. We observed slower proliferation and increased expression of genes involved in DNA metabolism and chromosome organization and maintenance in sRNA biogenesis mutants RSP1Δ, RDN2Δ, and RDF2Δ. In addition, RSP1Δ and RDN2Δ cells frequently exhibited enlarged chromatin extrusion bodies, which are nonnuclear, DNA-containing structures that may be akin to mammalian micronuclei. Expression of homologous recombination factor Rad51 was specifically elevated in RSP1Δ and RDN2Δ strains, with Rad51 and double-stranded DNA break marker γ-H2A.X localized to discrete macronuclear foci. In addition, an increase in Rad51 and γ-H2A.X foci was also found in knockouts of TWI8, a macronucleus-localized PIWI protein. Together, our findings suggest that an evolutionarily conserved role for RNAi pathways in maintaining genome integrity may be extended even to the early branching eukaryotic lineage that gave rise to Tetrahymena thermophila." @default.
- W3162690981 created "2021-05-24" @default.
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- W3162690981 date "2021-07-15" @default.
- W3162690981 modified "2023-10-16" @default.
- W3162690981 title "Disruption of a ∼23–24 nucleotide small RNA pathway elevates DNA damage responses in <i>Tetrahymena thermophila</i>" @default.
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- W3162690981 doi "https://doi.org/10.1091/mbc.e20-10-0631" @default.
- W3162690981 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34010017" @default.
- W3162690981 hasPublicationYear "2021" @default.
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