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• W3163242595 endingPage "119924" @default.
• W3163242595 startingPage "119924" @default.
• W3163242595 abstract "A convenient and high-throughput strategy for determining DNA methyltransferase activity has been developed. The strategy employs the efficient polymerization extension reaction catalyzed by terminal deoxynucleotidyl transferase and the copper nanoclusters with large Stokes shift to economically, rapidly and sensitively detect and quantify M.SssI activity. • Thanks to Cu NCs, the fluorescence intensity is enhanced and background is ignored. • Owing to TdTase, this assay has a wide detection range and good sensitivity. • The assay offers a signal amplification method applied to other biosensing systems. The assay of detecting DNA methyltransferase activity has been identified as one of the central challenges in cancer diagnostics as DNA methylation is closely related to the diagnosis and treatment of tumors. In this study, a label-free fluorescence probe based on poly-thymine copper nanoclusters engineered by terminal deoxynucleotidyl transferase is proposed for methyltransferase activity assay. Taking advantage of the efficient polymerization extension reaction catalyzed by terminal deoxynucleotidyl transferase and the copper nanoclusters with large Stokes shift instead of labeling fluorescent dyes, the strategy exhibits a broader linear scope from 1 to 300 U mL −1 with a detection limit of 0.176 U mL −1 . The economical method is specificity for M.SssI and also provides a convenient and high-throughput platform for screening the DNA methylation inhibitors, which displays a great potential for the practical applications of the drug development and clinical cancer diagnosis in the future." @default.
• W3163242595 created "2021-05-24" @default.
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• W3163242595 date "2021-11-01" @default.
• W3163242595 modified "2023-09-22" @default.
• W3163242595 title "Label-free fluorescence strategy for methyltransferase activity assay based on poly-thymine copper nanoclusters engineered by terminal deoxynucleotidyl transferase" @default.
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• W3163242595 doi "https://doi.org/10.1016/j.saa.2021.119924" @default.
• W3163242595 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33993023" @default.
• W3163242595 hasPublicationYear "2021" @default.
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