Matches in SemOpenAlex for { <https://semopenalex.org/work/W3163307812> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W3163307812 abstract "The acidic nuclear phosphoprotein 32 family member A (ANP32A) is a cellular host factor that determines the host tropism of the viral polymerase (vPol) of avian influenza viruses (AIVs). Compared with human ANP32A (hANP32A), chicken ANP32A contains an additional 33 amino acid residues (176–208) duplicated from amino acid residues 149–175 (27 residues), suggesting that these residues could be involved in increasing vPol activity by strengthening interactions between ANP32A and vPol. However, the molecular interactions and functional roles of the 27 residues within hANP32A during AIV vPol activity remain unclear. Here, we examined the functional role of 27 residues of hANP32A based on comparisons with other human (h) ANP32 family members. It was notable that unlike hANP32A and hANP32B, hANP32C could not support vPol activity or replication of AIVs, despite the fact that hANP32C shares a higher sequence identity with hANP32A than hANP32B. Pairwise comparison between hANP32A and hANP32C revealed that Asp149 (D149) and Asp152 (D152) are involved in hydrogen bonding and electrostatic interactions, respectively, which support vPol activity. Mutation of these residues reduced the interaction between hANP32A and vPol. Finally, we demonstrated that precise substitution of the identified residues within chicken ANP32A via homology-directed repair using the CRISPR/Cas9 system resulted in a marked reduction of viral replication in chicken cells. These results increase our understanding of ANP32A function and may facilitate the development of AIV-resistant chickens via precise modification of residues within ANP32A." @default.
- W3163307812 created "2021-05-24" @default.
- W3163307812 creator A5002858281 @default.
- W3163307812 creator A5013198280 @default.
- W3163307812 creator A5019323982 @default.
- W3163307812 creator A5046311928 @default.
- W3163307812 creator A5057441397 @default.
- W3163307812 creator A5059304389 @default.
- W3163307812 creator A5073663007 @default.
- W3163307812 creator A5076449366 @default.
- W3163307812 creator A5079923475 @default.
- W3163307812 creator A5083481821 @default.
- W3163307812 creator A5084548522 @default.
- W3163307812 creator A5087343618 @default.
- W3163307812 date "2021-05-13" @default.
- W3163307812 modified "2023-09-25" @default.
- W3163307812 title "Asp149 and Asp152 in chicken and human ANP32A play an essential role in the interaction with influenza viral polymerase" @default.
- W3163307812 cites W1518948347 @default.
- W3163307812 cites W1989063950 @default.
- W3163307812 cites W1990725338 @default.
- W3163307812 cites W2011226251 @default.
- W3163307812 cites W2012246068 @default.
- W3163307812 cites W2095336525 @default.
- W3163307812 cites W2099016746 @default.
- W3163307812 cites W2144104320 @default.
- W3163307812 cites W2224084604 @default.
- W3163307812 cites W2255501308 @default.
- W3163307812 cites W2470517214 @default.
- W3163307812 cites W2590493137 @default.
- W3163307812 cites W2752910994 @default.
- W3163307812 cites W2754553475 @default.
- W3163307812 cites W2766727330 @default.
- W3163307812 cites W2791952382 @default.
- W3163307812 cites W2897141255 @default.
- W3163307812 cites W2903201721 @default.
- W3163307812 cites W2909275889 @default.
- W3163307812 cites W2940065515 @default.
- W3163307812 cites W2948888443 @default.
- W3163307812 cites W2953156505 @default.
- W3163307812 cites W2964390716 @default.
- W3163307812 cites W2977640218 @default.
- W3163307812 doi "https://doi.org/10.1096/fj.202002006rr" @default.
- W3163307812 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33982347" @default.
- W3163307812 hasPublicationYear "2021" @default.
- W3163307812 type Work @default.
- W3163307812 sameAs 3163307812 @default.
- W3163307812 citedByCount "4" @default.
- W3163307812 countsByYear W31633078122022 @default.
- W3163307812 countsByYear W31633078122023 @default.
- W3163307812 crossrefType "journal-article" @default.
- W3163307812 hasAuthorship W3163307812A5002858281 @default.
- W3163307812 hasAuthorship W3163307812A5013198280 @default.
- W3163307812 hasAuthorship W3163307812A5019323982 @default.
- W3163307812 hasAuthorship W3163307812A5046311928 @default.
- W3163307812 hasAuthorship W3163307812A5057441397 @default.
- W3163307812 hasAuthorship W3163307812A5059304389 @default.
- W3163307812 hasAuthorship W3163307812A5073663007 @default.
- W3163307812 hasAuthorship W3163307812A5076449366 @default.
- W3163307812 hasAuthorship W3163307812A5079923475 @default.
- W3163307812 hasAuthorship W3163307812A5083481821 @default.
- W3163307812 hasAuthorship W3163307812A5084548522 @default.
- W3163307812 hasAuthorship W3163307812A5087343618 @default.
- W3163307812 hasConcept C104317684 @default.
- W3163307812 hasConcept C159047783 @default.
- W3163307812 hasConcept C54355233 @default.
- W3163307812 hasConcept C82381507 @default.
- W3163307812 hasConcept C86803240 @default.
- W3163307812 hasConceptScore W3163307812C104317684 @default.
- W3163307812 hasConceptScore W3163307812C159047783 @default.
- W3163307812 hasConceptScore W3163307812C54355233 @default.
- W3163307812 hasConceptScore W3163307812C82381507 @default.
- W3163307812 hasConceptScore W3163307812C86803240 @default.
- W3163307812 hasFunder F4320322120 @default.
- W3163307812 hasIssue "6" @default.
- W3163307812 hasLocation W31633078121 @default.
- W3163307812 hasOpenAccess W3163307812 @default.
- W3163307812 hasPrimaryLocation W31633078121 @default.
- W3163307812 hasRelatedWork W1662183131 @default.
- W3163307812 hasRelatedWork W2071687412 @default.
- W3163307812 hasRelatedWork W2078796009 @default.
- W3163307812 hasRelatedWork W2092274155 @default.
- W3163307812 hasRelatedWork W2148544165 @default.
- W3163307812 hasRelatedWork W2158783068 @default.
- W3163307812 hasRelatedWork W2329249948 @default.
- W3163307812 hasRelatedWork W2330530703 @default.
- W3163307812 hasRelatedWork W2575306312 @default.
- W3163307812 hasRelatedWork W4253008530 @default.
- W3163307812 hasVolume "35" @default.
- W3163307812 isParatext "false" @default.
- W3163307812 isRetracted "false" @default.
- W3163307812 magId "3163307812" @default.
- W3163307812 workType "article" @default.