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• W3163629249 abstract "Abstract Background Inappropriate matching of motor and sensory fibers after nerve repair or nerve grafting can lead to failure of nerve recovery. Identification of motor and sensory fibers is important for the development of new approaches that facilitate neural regeneration and the next generation of nerve signal-controlled neuro-prosthetic limbs with sensory feedback technology. Only a few methods have been reported to differentiate sensory and motor nerve fascicles, and the reliability of these techniques is unknown. Immunofluorescence staining is one of the most commonly used methods to distinguish sensory and motor nerve fibers, however, its accuracy remains unknown. Methods In this study, we aim to determine the efficacy of popular immunofluorescence markers for motor and sensory nerve fibers. We harvested the facial (primarily motor fascicles) and sural (primarily sensory fascicles) nerves in rats, and examined the immunofluorescent staining expressions of motor markers (choline acetyltransferase (ChAT), tyrosine kinase (TrkA)), and sensory markers [neurofilament protein 200 kDa (NF-200), calcitonin gene-related peptide (CGRP) and Transient receptor potential vanillic acid subtype 1 (TRPV1)]. Three methods, including the average area percentage, the mean gray value, and the axon count, were used to quantify the positive expression of nerve markers in the immunofluorescence images. Results Our results suggest the mean gray value method is the most reliable method. The mean gray value of immunofluorescence in ChAT (63.0 ± 0.76%) and TRKA (47.6 ± 0.43%) on the motor fascicles was significantly higher than that on the sensory fascicles (ChAT: 49.2 ± 0.72%, P < 0.001; and TRKA: 29.1 ± 0.85%, P < 0.001). Additionally, the mean gray values of TRPV1 (51.5 ± 0.83%), NF-200 (61.5 ± 0.62%) and CGRP (37.7 ± 1.22%) on the motor fascicles were significantly lower than that on the sensory fascicles respectively (71.9 ± 2.32%, 69.3 ± 0.46%, and 54.3 ± 1.04%) (P < 0.001). The most accurate cutpoint occurred using CHAT/CRCP ratio, where a value of 0.855 had 100% sensitivity and 100% specificity to identify motor and sensory nerve with an area under the ROC curve of 1.000 (P < 0.001). Conclusions A combination of ChAT and CGRP is suggested to distinguish motor and sensory nerve fibers." @default.
• W3163629249 created "2021-05-24" @default.
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• W3163629249 date "2021-05-13" @default.
• W3163629249 modified "2023-10-14" @default.
• W3163629249 title "Identification of sensory and motor nerve fascicles by immunofluorescence staining after peripheral nerve injury" @default.
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• W3163629249 cites W1834115420 @default.
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• W3163629249 cites W1975042569 @default.
• W3163629249 cites W1978488098 @default.
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• W3163629249 cites W1998238515 @default.
• W3163629249 cites W2023943079 @default.
• W3163629249 cites W2031656814 @default.
• W3163629249 cites W2038381731 @default.
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• W3163629249 cites W2079747710 @default.
• W3163629249 cites W2118357539 @default.
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• W3163629249 cites W2120331067 @default.
• W3163629249 cites W2121480525 @default.
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• W3163629249 cites W2272396139 @default.
• W3163629249 cites W2336033976 @default.
• W3163629249 cites W2405405482 @default.
• W3163629249 cites W2409565129 @default.
• W3163629249 cites W2474182806 @default.
• W3163629249 cites W2523252831 @default.
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• W3163629249 doi "https://doi.org/10.1186/s12967-021-02871-w" @default.
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