Matches in SemOpenAlex for { <https://semopenalex.org/work/W3163702023> ?p ?o ?g. }
- W3163702023 endingPage "3387" @default.
- W3163702023 startingPage "3365" @default.
- W3163702023 abstract "The vast majority of plant viruses are transmitted by insect vectors, with many crucial aspects of the transmission process being mediated by key protein–protein interactions. Still, very few vector proteins interacting with viruses have been identified and functionally characterized. Potato leafroll virus (PLRV) is transmitted most efficiently by Myzus persicae, the green peach aphid, in a circulative, non-propagative manner. Using affinity purification coupled to high-resolution mass spectrometry (AP–MS), we identified 11 proteins from M. persicaedisplaying a high probability of interaction with PLRV and an additional 23 vector proteins with medium confidence interaction scores. Three of these aphid proteins were confirmed to directly interact with the structural proteins of PLRV and other luteovirid species via yeast two-hybrid. Immunolocalization of one of these direct PLRV-interacting proteins, an orthologue of the human innate immunity protein complement component 1 Q subcomponent-binding protein (C1QBP), shows that MpC1QBP partially co-localizes with PLRV in cytoplasmic puncta and along the periphery of aphid gut epithelial cells. Artificial diet delivery to aphids of a chemical inhibitor of C1QBP leads to increased PLRV acquisition by aphids and subsequently increased titer in inoculated plants, supporting a role for C1QBP in the acquisition and transmission efficiency of PLRV by M. persicae. This study presents the first use of AP–MS for the in vivo isolation of a functionally relevant insect vector–virus protein complex. MS data are available from ProteomeXchange.org using the project identifier PXD022167." @default.
- W3163702023 created "2021-05-24" @default.
- W3163702023 creator A5006136242 @default.
- W3163702023 creator A5008666950 @default.
- W3163702023 creator A5009086308 @default.
- W3163702023 creator A5013250394 @default.
- W3163702023 creator A5023538941 @default.
- W3163702023 creator A5026671931 @default.
- W3163702023 creator A5043959168 @default.
- W3163702023 creator A5091733369 @default.
- W3163702023 date "2021-05-21" @default.
- W3163702023 modified "2023-10-10" @default.
- W3163702023 title "Affinity Purification–Mass Spectrometry Identifies a Novel Interaction between a Polerovirus and a Conserved Innate Immunity Aphid Protein that Regulates Transmission Efficiency" @default.
- W3163702023 cites W1494638726 @default.
- W3163702023 cites W1551677269 @default.
- W3163702023 cites W1792671145 @default.
- W3163702023 cites W1821479442 @default.
- W3163702023 cites W1895300367 @default.
- W3163702023 cites W1902182170 @default.
- W3163702023 cites W1946909819 @default.
- W3163702023 cites W1966064695 @default.
- W3163702023 cites W1967493968 @default.
- W3163702023 cites W1968578271 @default.
- W3163702023 cites W1977997994 @default.
- W3163702023 cites W1978891398 @default.
- W3163702023 cites W1982032059 @default.
- W3163702023 cites W1982629607 @default.
- W3163702023 cites W1987060243 @default.
- W3163702023 cites W1989144074 @default.
- W3163702023 cites W1994412627 @default.
- W3163702023 cites W1994614818 @default.
- W3163702023 cites W1998121141 @default.
- W3163702023 cites W1998740202 @default.
- W3163702023 cites W2000960719 @default.
- W3163702023 cites W2002110104 @default.
- W3163702023 cites W2005044790 @default.
- W3163702023 cites W2005367448 @default.
- W3163702023 cites W2006902688 @default.
- W3163702023 cites W2023124677 @default.
- W3163702023 cites W2028975567 @default.
- W3163702023 cites W2040025563 @default.
- W3163702023 cites W2040678235 @default.
- W3163702023 cites W2044504978 @default.
- W3163702023 cites W2047077861 @default.
- W3163702023 cites W2052723491 @default.
- W3163702023 cites W2053004210 @default.
- W3163702023 cites W2055761917 @default.
- W3163702023 cites W2057586293 @default.
- W3163702023 cites W2058561364 @default.
- W3163702023 cites W2078726734 @default.
- W3163702023 cites W2083619732 @default.
- W3163702023 cites W2088888594 @default.
- W3163702023 cites W2089667868 @default.
- W3163702023 cites W2093534373 @default.
- W3163702023 cites W2101614917 @default.
- W3163702023 cites W2102716555 @default.
- W3163702023 cites W2103811513 @default.
- W3163702023 cites W2104623118 @default.
- W3163702023 cites W2109142806 @default.
- W3163702023 cites W2113471433 @default.
- W3163702023 cites W2117275461 @default.
- W3163702023 cites W2117976997 @default.
- W3163702023 cites W2118059471 @default.
- W3163702023 cites W2121588384 @default.
- W3163702023 cites W2122678977 @default.
- W3163702023 cites W2125283541 @default.
- W3163702023 cites W2129067001 @default.
- W3163702023 cites W2132152710 @default.
- W3163702023 cites W2135999995 @default.
- W3163702023 cites W2141091417 @default.
- W3163702023 cites W2141429272 @default.
- W3163702023 cites W2145625192 @default.
- W3163702023 cites W2153603251 @default.
- W3163702023 cites W2159906800 @default.
- W3163702023 cites W2160110467 @default.
- W3163702023 cites W2162519820 @default.
- W3163702023 cites W2162544781 @default.
- W3163702023 cites W2163658976 @default.
- W3163702023 cites W2166570887 @default.
- W3163702023 cites W2167844024 @default.
- W3163702023 cites W2169781776 @default.
- W3163702023 cites W2171789846 @default.
- W3163702023 cites W2322789729 @default.
- W3163702023 cites W2337274641 @default.
- W3163702023 cites W2385669458 @default.
- W3163702023 cites W2538263254 @default.
- W3163702023 cites W2548860498 @default.
- W3163702023 cites W2550558709 @default.
- W3163702023 cites W2559932527 @default.
- W3163702023 cites W2612777778 @default.
- W3163702023 cites W2617436115 @default.
- W3163702023 cites W2736564769 @default.
- W3163702023 cites W2760227079 @default.
- W3163702023 cites W2766699253 @default.
- W3163702023 cites W2790538575 @default.
- W3163702023 cites W2791214754 @default.
- W3163702023 cites W2800153966 @default.
- W3163702023 cites W2803682532 @default.