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- W3164133047 abstract "Untargeted metabolomics has rapidly become a profiling method of choice in many areas of research, including mitochondrial biology. Most commonly, untargeted metabolomics is performed with liquid chromatography/mass spectrometry because it enables measurement of a relatively wide range of physiochemically diverse molecules. Specifically, to assess energy pathways that are associated with mitochondrial metabolism, hydrophilic interaction liquid chromatography (HILIC) is often applied before analysis with a high-resolution accurate mass instrument. The workflow produces large, complex data files that are impractical to analyze manually. Here, we present a protocol to perform untargeted metabolomics on biofluids such as plasma, urine, and cerebral spinal fluid with a HILIC separation and an Orbitrap mass spectrometer. Our protocol describes each step of the analysis in detail, from preparation of solvents for chromatography to selecting parameters during data processing." @default.
- W3164133047 created "2021-06-07" @default.
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- W3164133047 date "2021-01-01" @default.
- W3164133047 modified "2023-10-12" @default.
- W3164133047 title "A Protocol for Untargeted Metabolomic Analysis: From Sample Preparation to Data Processing" @default.
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- W3164133047 doi "https://doi.org/10.1007/978-1-0716-1266-8_27" @default.
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