Matches in SemOpenAlex for { <https://semopenalex.org/work/W3164581672> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W3164581672 endingPage "2100135" @default.
- W3164581672 startingPage "2100135" @default.
- W3164581672 abstract "A novel protein (D1 component) was purified from Boletus bicolor by ion-exchange chromatography and gel chromatography on a HiTrap™ Q HP column, a diethylaminoethanol cellulose-52 column, and a Sephadex G75 column, respectively. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the D1 component was a single protein band with a molecular weight of about 40 kDa. The sulforhodamine B assay showed that at concentrations as low as 25-75 μg/ml, protein D1 significantly inhibited the proliferation of human lung adenocarcinoma cell lines (A549 and H1299 cells) and had little effect on human normal kidney cells (HEK293 cells). After labeling protein D1, it was found that D1 could enter the cytoplasm of tumor cells and colocated with lysosomes. Flow cytometry results demonstrated that protein D1 induced apoptosis and G1 phase arrest of the cell cycle in A549 and H1299 cells. The Western blot analysis results showed that the expression of apoptosis and cell cycle-related proteins of cleaved caspase-3, cytochrome c, Bax, P16, and P21 was significantly upregulated, whereas the expression of Bcl-2, CDK4, cyclin D, p-Rb, and E2F was significantly downregulated after treatment with protein D1. Therefore, D1 exhibits potential to be developed into an antitumor agent." @default.
- W3164581672 created "2021-06-07" @default.
- W3164581672 creator A5003987097 @default.
- W3164581672 creator A5010169013 @default.
- W3164581672 creator A5035780833 @default.
- W3164581672 creator A5058026071 @default.
- W3164581672 date "2021-06-02" @default.
- W3164581672 modified "2023-09-26" @default.
- W3164581672 title "Purification of a novel protein with cytotoxicity against non‐small‐cell lung cancer cells from <i>Boletus bicolor</i>" @default.
- W3164581672 cites W1650797637 @default.
- W3164581672 cites W1965275923 @default.
- W3164581672 cites W1982287057 @default.
- W3164581672 cites W1982791294 @default.
- W3164581672 cites W1985587577 @default.
- W3164581672 cites W1991298304 @default.
- W3164581672 cites W1991494214 @default.
- W3164581672 cites W2004142170 @default.
- W3164581672 cites W2022165024 @default.
- W3164581672 cites W2034699067 @default.
- W3164581672 cites W2065955521 @default.
- W3164581672 cites W2067791199 @default.
- W3164581672 cites W2070104396 @default.
- W3164581672 cites W2077813807 @default.
- W3164581672 cites W2079709377 @default.
- W3164581672 cites W2091334821 @default.
- W3164581672 cites W2094614534 @default.
- W3164581672 cites W2095588202 @default.
- W3164581672 cites W2103441704 @default.
- W3164581672 cites W2104504726 @default.
- W3164581672 cites W2158234609 @default.
- W3164581672 cites W2164785556 @default.
- W3164581672 cites W2466033276 @default.
- W3164581672 cites W2589500528 @default.
- W3164581672 cites W2767281692 @default.
- W3164581672 cites W2770414886 @default.
- W3164581672 cites W2784371446 @default.
- W3164581672 cites W2889646458 @default.
- W3164581672 cites W2972974089 @default.
- W3164581672 cites W3009963727 @default.
- W3164581672 cites W3025811668 @default.
- W3164581672 doi "https://doi.org/10.1002/ardp.202100135" @default.
- W3164581672 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34076300" @default.
- W3164581672 hasPublicationYear "2021" @default.
- W3164581672 type Work @default.
- W3164581672 sameAs 3164581672 @default.
- W3164581672 citedByCount "4" @default.
- W3164581672 countsByYear W31645816722023 @default.
- W3164581672 crossrefType "journal-article" @default.
- W3164581672 hasAuthorship W3164581672A5003987097 @default.
- W3164581672 hasAuthorship W3164581672A5010169013 @default.
- W3164581672 hasAuthorship W3164581672A5035780833 @default.
- W3164581672 hasAuthorship W3164581672A5058026071 @default.
- W3164581672 hasConcept C104317684 @default.
- W3164581672 hasConcept C153911025 @default.
- W3164581672 hasConcept C185592680 @default.
- W3164581672 hasConcept C190283241 @default.
- W3164581672 hasConcept C199835354 @default.
- W3164581672 hasConcept C2776415932 @default.
- W3164581672 hasConcept C29537977 @default.
- W3164581672 hasConcept C54355233 @default.
- W3164581672 hasConcept C55493867 @default.
- W3164581672 hasConcept C81885089 @default.
- W3164581672 hasConcept C86803240 @default.
- W3164581672 hasConceptScore W3164581672C104317684 @default.
- W3164581672 hasConceptScore W3164581672C153911025 @default.
- W3164581672 hasConceptScore W3164581672C185592680 @default.
- W3164581672 hasConceptScore W3164581672C190283241 @default.
- W3164581672 hasConceptScore W3164581672C199835354 @default.
- W3164581672 hasConceptScore W3164581672C2776415932 @default.
- W3164581672 hasConceptScore W3164581672C29537977 @default.
- W3164581672 hasConceptScore W3164581672C54355233 @default.
- W3164581672 hasConceptScore W3164581672C55493867 @default.
- W3164581672 hasConceptScore W3164581672C81885089 @default.
- W3164581672 hasConceptScore W3164581672C86803240 @default.
- W3164581672 hasFunder F4320321001 @default.
- W3164581672 hasIssue "9" @default.
- W3164581672 hasLocation W31645816721 @default.
- W3164581672 hasLocation W31645816722 @default.
- W3164581672 hasOpenAccess W3164581672 @default.
- W3164581672 hasPrimaryLocation W31645816721 @default.
- W3164581672 hasRelatedWork W1518035471 @default.
- W3164581672 hasRelatedWork W2239557550 @default.
- W3164581672 hasRelatedWork W2359296231 @default.
- W3164581672 hasRelatedWork W2363295569 @default.
- W3164581672 hasRelatedWork W2383837582 @default.
- W3164581672 hasRelatedWork W2386955390 @default.
- W3164581672 hasRelatedWork W2387700717 @default.
- W3164581672 hasRelatedWork W2390533288 @default.
- W3164581672 hasRelatedWork W2472820331 @default.
- W3164581672 hasRelatedWork W2555524919 @default.
- W3164581672 hasVolume "354" @default.
- W3164581672 isParatext "false" @default.
- W3164581672 isRetracted "false" @default.
- W3164581672 magId "3164581672" @default.
- W3164581672 workType "article" @default.