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- W3164623800 abstract "Abstract Spatial analysis of microbiomes at single cell resolution with high multiplexity and accuracy has remained challenging. Here we present spatial profiling of a microbiome using sequential error-robust fluorescence in situ hybridization (SEER-FISH), a highly multiplexed and accurate imaging method that allows mapping of microbial communities at micron-scale. We show that multiplexity of RNA profiling in microbiomes can be increased significantly by sequential rounds of probe hybridization and dissociation. Combined with error-correction strategies, we demonstrate that SEER-FISH enables accurate taxonomic identification in complex microbial communities. Using microbial communities composed of diverse bacterial taxa isolated from plant rhizospheres, we show that SEER-FISH can quantify the abundance of each taxon and map microbial biogeography on roots. SEER-FISH provides an unprecedented method for profiling the spatial ecology of complex microbial communities in situ." @default.
- W3164623800 created "2021-06-07" @default.
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- W3164623800 date "2021-07-15" @default.
- W3164623800 modified "2023-09-28" @default.
- W3164623800 title "Spatial profiling of microbial communities by sequential FISH with error-robust encoding" @default.
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- W3164623800 doi "https://doi.org/10.21203/rs.3.rs-601828/v1" @default.
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